Photosynthesis Lesson 1 Outcomes 1. Explain how energy is absorbed by pigments, transferred through the reduction of NADP+ to NADPH, and then transferred as chemical potential energy to ATP by chemiosmosis 2. Explain how NADPH and ATP are used to reduce carbon in the light independent reactions for the production of glucose 3. Identify where in the chloroplast these steps occur ATP What is ATP? Universal ______________ molecule Energy in a form the cell can use Makes energy readily _________________ Continuously being remade Stands for ___________________ _________________________ Uses of ATP _______________ ___________________ of ions and molecules ________________molecules Switching ______________ on or off Redox Reactions Redox reactions (__________________-________________ reactions happen throughout photosynthesis as _______________ get transferred from one molecule to another. There are a couple of ways to remember what happens to the electron o OIL RIG (_________________ is _____________, _______________ is _________________) o ______ goes _______ (lose electrons=oxidation, gain electrons=reduction) This is just a _____________ _______________ of gaining electrons (___) and passing them to the next compound. Photosynthesis The process of storing __________ energy (photons) as ____________ energy (carbohydrates) in the cell. The equation: Photosynthesis occurs in _____________________ Chloroplast parts ______________ o I disk o Granum = a stack of disks o Grana = plural granum (many stacks of disks) o Site of the light dependent reaction (holds the ______________) ____________ o Gel surrounding the thylakoids o Site of the light independent reaction Chlorophyll A ___________ chemical that traps sunlight energy Located in the __________ of a chloroplast The ___________ chlorophyll a and chlorophyll b absorb light wavelengths on the _____ and ______ ends of the spectrum They ____________ green light, that’s why plants look green. Pigments Leaves contain other light _______________ pigments (chemicals) besides chlorophyll o Carotenoids (including carotene which reflects orange light, xanthophyll which reflects yellow light, and lycopene which reflects red light) o Flavonoids (more yellow reflecting pigments, and anthocyanin which reflects shades of red, blue, and purple) Different plants have different _____________________ of these chemicals In the fall, chlorophylls begin to break down, and the other reflected colours begin to ________ through with the green to overpower them. Lesson 1 Formative Do the following questions and send them to me to check for understanding. Pg 181 #2, pg 182 #4, pg 185 #4 Lesson 2 Photosynthesis There are two main parts to photosynthesis o ________ ____________________ _______________ Needs light, therefore it takes place in the thylakoid _______________ where the chlorophyll is Takes in ________ and creates oxygen as a _____________. Creates intermediary (not _______) products of _______ and ________ for the Calvin Cycle. o _________ ___________________ _______________ Doesn’t need ________ so it happens outside the thylakoid in the _________ Also referred to as the _________ reactions (misleading because it doesn’t only happen in the dark) Takes in _____ and _______ and ___________ from the thylakoid to make __________ in a process called the Calvin Cycle. Light Dependent Reaction All of these steps are happening at the same time, but for simplicity’s sake, we will learn them separately. 1. Photosystem II (PSII) o PSII is a group of chlorophyll pigments located on the ___________ ___________. o It absorbs the ____________ energy, transforming it into chemical energy in the form of excited electrons ( ) o These are unstable and ________ the chlorophyll, attaching to molecules in the _________ (Electron Transport Chain) o The chlorophyll loses its electrons so it is _____________ (remember LEO) 2. Photolysis o In order to replace the electrons lost from the chlorophyll, a _______ molecule will be split apart. o ___________ energy is used to split H2O into its parts The __________ now leaves the thylakoid and the chloroplast and goes into the air for us to breathe The H splits into its H+ and its electron (e-) The e- goes to the _____________ to replace the it lost The chlorophyll has gained more electrons, therefore it has been ____________ 3. Electron Transport Chain (ETC) o The from the PSII are transferred through the _______ like a bucket o o o o o o o brigade (one molecule ___________ it, and then passes it onto the next molecule) This is a series of ________ ___________, as the molecule that accepts the is being ____________, but then is oxidized when it passes the onto the next molecule When the _____ are being transferred, some of their _________ is used to take H+ that are floating around in the stroma and ________ them into the thylakoid ________ The pumping of H+ into the thylakoid is an example of _______ _____________ This means it is using _____________ from the and the movement is against the concentration gradient The H+ are being pumped from an area of ______ concentration to an area of ________ concentration Building up a H+ concentration gradient means that the ________ of the thylakoid is more ______________ charged than the stroma. This is a form of ________________ energy (electrochemical gradient) The _______ their excess energy as they are transferred in the ETC until they reach Photosystem I (PSI) 4. Reduction of NADP+ o When the low energy e- reaches PSI it gets __________ by the sun (e- into o It leaves the ___________ at PSI and joins with ____ and _________ (floating ) around waiting to be reduced) in the stroma to make __________ o NADPH is an electron __________. It moves the and its H+ from the thylakoid to the ____________ __________. 5. Chemiosmosis o This is the process of making ATP using the potential electrochemical energy o o o o from the H+ __________________ ____________________ The _____ concentration has built up in the ____________ lumen thanks to the active transport of H+ inside by the ETC. The _________ concentration of H+ means that they want to ___________ out in order to achieve ____________________. The H+ diffuse across the membrane because they are ______________, so they must use a protein channel (facilitated diffusion) The protein channel they diffuse out through is called the _______ ______________ _______________. o As the H+ _________________ through the ATP synthase channel, the structure turns the potential energy (from H+ gradient) into ___________ energy that turns the protein channel o This kinetic energy is then transferred to the ____________ bond between ADP and P to make the energy rich compound _____ Light Independent Reaction This takes place in the _________ of the chloroplast It is also called the ____________ ___________ In this step, the CO2 from the air is bonded to the and the H+ from NADPH to make glucose (___________) – Carbon Fixation o Oxidizing NADPH back into NADP+ ATP donates its _____________ chemical energy to bond the C, H, and O together by _____________ apart into ADP and P. Now you have the final product of ______________ And the ADP, P, and NADP+ can go back to the ___________ to be used in the Light Dependent Reactions again. o They continue to cycle back and forth between the main stages Summary Light energy is absorbed by __________________ and turned into chemical energy by exciting electrons Water is ______________ apart to replace lost e- in PSII The electrons’ energy is used to create a ____ ___________________ ______________ (electrochemical potential energy now) in the ETC This gradient in turn transfers the energy into ____________ chemical energy in the ATP molecule when H+ ___________ through the ATP Synthase Complex The electrons absorb more ______________ energy and are carried from the thylakoid to the Calvin Cycle by _________ The energy from _______ bonds the e- and H+ from NADPH to the CO2 to make C6H12O6 Lesson 2 Formative Do the following questions for practice to see where you need to review more. Pg 187 #1-3, pg 188 #4-6, pg 190 # 7-10, pg 191 #11-14, pg 193 #15-16 Then create a summary of photosynthesis from the perspective of an electron. You can write a story, song, poem, etc., you can draw it out like a comic book or poster, or you can make a computer animation…whatever format you want. Send the summary to me before you write your test so that I can see if you understand the process.