Activity: Buffer Solutions We know that a buffer is defined as a solution that can resist a change in pH, even when small amounts of strong acid or base is added to it. We also know that a buffer solution must contain approximately equal amounts of a conjugate acid-base pair. Let’s test this out! PROCEDURE: First we need to create the buffer. We will do this by adding a small amount of strong acid (HCl(aq)) to a weak base (sodium acetate). 1. Measure 20.0mL of sodium acetate in a graduated cylinder and then transfer the sodium acetate solution into a 50mL beaker. 2. Using a graduated pipette, measure out 2.0mL of HCl(aq) and add this to the 20.0mL of sodium acetate in the 50mL beaker. We should now have a buffer solution. Now let’s test our buffer compared to water. 3. Using a graduate cylinder, measure out 10mL of buffer solution and transfer it to a 50 mL beaker. 4. Add several drops of universal indicator to the sample. 5. Using a disposable pipette, count how many drops of NaOH(aq) can be added to the buffer solution until a significant color change is observed? Drops of NaOH(aq) added to buffer:______________ 6. Rinse solution down the drain and rinse 50mL beaker with water. 7. Repeat steps 3-6 but with water instead of a buffer solution and compare how many drops of NaOH(aq) can be added to water before a significant color change is observed. Drops of NaOH(aq) added to water:______________ 8. Using a graduate cylinder, measure out a new 10mL of buffer solution and transfer into a 50mL beaker. 9. Add several drops of thymol blue indicator to the sample. 10. Using a disposable pipette, count how many drops of HCl(aq) can be added to the buffer solution until a significant color change is observed. Drops of HCl(aq) added to buffer:______________ 11. Rinse solution down the drain and rinse 50mL beaker with water. 12. Repeat steps 8-11 but with water instead of a buffer solution and compare how many drops of HCl(aq) can be added to water before a significant color change is observed. Drops of HCl(aq) added to water:______________