OHS026
Safe Work Procedure
Faculty/Division
Medicine
Document number
SOMS_IIR_SWP25
School/ Divisional Unit
SOMS
Initial Issue date
March 2010
Current version
1.1
Current Version
Issue date
March 2010
Next review date
March 2011
The Writing Safe Work Procedures Guideline (OHS027) should be consulted to assist in the completion of this
form.
Safe Work Procedure Title and basic description
Title: Ultraviolet Crosslinkers
Description: Expose samples to a controlled amount of ultraviolet radiation.
CL-1000 Shortwave Crosslinker and CL-1000L Longwave Crosslinker are located in Rm524.
Associated risk assessment title and location:
Describe the activity or process
IMPORTANT: Please read the Operating Instrucitons and Service Manual before operating your UVP UV Crosslinker to
familiarize yourself with its operation.
General Operation Procedure:
1.
2.
3.
4.
5.
6.
Place the Crosslinker on a level work surface. Be sure there is enough room in front to open the door.
The UV Crosslinker is connected to a properly grounded electrical outlet.
Turn the ON/OFF switch to the ON position.
The last UV exposure setting will now be displayed on the LED.
Place your sample requiring exposure into the chamber.
Preset the equipment to the requiring condition.
Note: please read the manual book to know how to preset the equipment.
7. Push START.
8. After a slight delay to energize the UV tubes, the LED will begin to countdown. The unit will stop automatically at the
end of the exposure cycle and will beep five times. Exposure is now complete.
Note: do not open the door until procedure is complete.
9. At the end of the exposure cycle, simply open the door and remove your sample.
10. Clean up the surface with soft cloth. Please do not leave any chemicals on unit surfaces.
Applications:

UV crosslinking of DNA and RNA to nitrocellulose, nylon or reinforced nitrocellulose

PCR sample contamination control

Photoniking of DNA

Testing RecA function

Rapid site mapping

Protein crosslinking

Not suitable for animal use
List all resources required including plant,
chemicals, personal protective clothing and
equipment, etc
Must wear gloves and laboratory coat.
UV protective glasses (optional).
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Page 1 of 2
Safe Work Procedure
Uncontrolled document when printed
Date Effective: 01/01/2007
Current Version: 1.2, 15/08/2007
List potential hazards and risk controls including
specific precautions required






Ultraviolet Crosslinkers are a powerful source of ultraviolet radiation.
Do not attempt to disengage or override the internal safety interlocks. Exposure to the UV radiation may result.
Do not expose unprotected eyes or skin to UV radiation.
Do not open the door until the procedure is complete.
If the UV sources remain on when the door is open, the unit is malfunctioning and use should be discontinued until the unit
is serviced.
Always disconnect the UV Crosslinkers from its electrical supply before serving.
List emergency shutdown instructions
In the event of an electrical hazard, turn off equipment at the power.
Inform the equipment custodian.
For major emergencies dial x56666
List clean up and waste disposal requirements
Note: Always unplug unit from electrical supply before cleaning or drying.
Maintenance, Care and Cleaning of the Crosslinkers:

Wipe ANY water from inside and outside the unit with a soft cloth or sponge.

Use soap and water with a soft cloth or sponge to clean the unit.

Do not allow chemicals to remain on unit surfaces.

Do not leave any waste or container inside the unit.

Never clean unit with abrasive pads or cleaners.

Never clean unit with acetone or chloroform.

Clean the UV sensor regularly with a soft cloth and alcohol.
List legislation, standards and codes of practice used
in the development of the SWP
NSW OHS Act 2000
NSW OHS Regulation 2001
Gene Technology Act 2000
Gene Technology Regulation 2001
Gene Technology (NSW) Act 2003
Handbook on the Regulation of Gene Technology in Australia
AS/NZS 2243.1:2005 Australian/New Zealand Standard™ Safety in laboratories Part 1: Planning and operational aspects
AS/NZS 2243.2:2006 Australian/New Zealand Standard™ Safety in laboratories Part 2: Chemical aspects
AS 2243.6—1990 Australian Standard Safety in laboratories Part 7: Electrical aspects
AS 2243.3—2000 Australian/New Zealand Standard Safety in laboratories Part 3: Microbiological aspects and containment facilities
Supervisory approval, training, and review
Supervisor: Nicodemus Tedla
Signature:
Plant custodian: Hongyan An
Signature
List competency required – qualifications, certificates, licencing, training - eg course or instruction:

UNSW Laboratory Safety Awareness training

UNSW Occupational Health & Safety Awareness training

UNSW Hazardous Substances training

Training in use of the equipment by the plant custodian
SWP review date: 16-03-2010
Responsibility for SWP review: Hongyan An
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Page 2 of 2
Safe Work Procedure
Uncontrolled document when printed
Date Effective: 01/01/2007
Current Version: 1.2, 15/08/2007