OHS026
Safe Work Procedure
Faculty/Division
School/ Divisional Unit
SCHOOL OF MEDICAL SCIENCES
MEDICINE
Document number
Initial Issue date
28-4-10
Current version
1
Current Version
Issue date
Next review date
24-4-2013
SOMS GBD SWP
The Writing Safe Work Procedures Guideline (OHS027) should be consulted to assist in the completion of
this form.
Safe Work Procedure Title and basic
description
Title: Colorimetric detection of signal of DIG labelled probe.
Description: Detection of signal of Hybridization of DIG labelled probe to cellular mRNA using commercial
Roche kit.
Associated risk assessment title and location:
Describe the activity or process
- Leave slides in Coplin Jars after stringency wash.
- Wash slides (or membrane) in 1xWashing buffer.
- Incubate slides (membrane) in 1xBlocking buffer RT 1hr containing Anti-DIG-Ab, RT, 1hr.
- Incubate slides (membrane) in 1xBlocking buffer containing Anti-DIG-Ab, RT, 1hr
- Wash slides (membrane) with Washing buffer RT, 2x15min
- Equilibrate in 1XDetection buffer.
- Develop in NBT/BCIP substrate 1.5hr to ON in the dark.
List all resources required including plant,
chemicals, personal protective clothing and
equipment, etc
Lab gown, closed shoes, gloves;
RNase free reagents; tips, pipettes and glass equipment’
Coplin jars.
Plastic “Blue lid” 50ml tubes for dilutions.
Wax Daco Pen.
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Page 1 of 2
Safe Work Procedure
Date Effective: 01/01/2007
Uncontrolled document when printed
Current Version: 1.2, 15/08/2007
List potential hazards and risk controls
including specific precautions required
 DIG kit reagents can cause irritation to eyes and skin by contact or inhalation. Wear gloves and
gown and safety glasses when handling the kit. Must read MSDS FOR KITS before the start of the
experiment.
 Use Eye wash and shower station if contact occurs.
 NBT/BCIP substrate is light sensitive must be kept in the dark during developing step.
List emergency shutdown instructions
In the event of inhalation or ingestion the first aid officer should be consulted immediately.
First Aid Officer: Renee Szokolai ext 58497
List clean up and waste disposal requirements
All tips and tubes are disposed into biological waste bins.
All solutions are safe to pour down the sink. Flush with water.
List legislation, standards and codes of practice
used in the development of the SWP
NSW OHS Act 2000
NSW OHS Regulation 2001
Code of Practice for the Labelling of Workplace Substances
AS/NZS 2243.2:2006. Safety in laboratories. Part 2: Chemical aspects
AS/NZS 2243.3: 2006 Safety in laboratories Part 3: Microbiological aspects and containment facilities
AS/NZS 1336:1997 Recommended Practices for Occupational Eye Protection
AS/NZS 2161.1:2000 Occupational Protective Gloves – Selection, Use and Maintenance
Supervisory approval, training, and review
Supervisor: Prof E Burcher
Signature:
Plant custodian:
Signature
List competency required – qualifications, certificates, licensing, training - e.g. course or instruction:
COMMON SENSE
SWP review date: 24-42013
Responsibility for SWP review: Fei Shang
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Page 2 of 2
Safe Work Procedure
Date Effective: 01/01/2007
Uncontrolled document when printed
Current Version: 1.2, 15/08/2007