ESM 1 Details of the sampling sites for (i) the artificial substrates experiment and (ii) the
study of native bivalve infestation by Dreissena (localities 1 to 6). The codes in the first
column are in accordance with the map of Fig. 1
Localities
1
2
3
4
5
6
La Plante (Be)
Waulsort (Be)
Givet (Fr)
Monthermé (Fr)
Amée
Dave
Godinne
Dinant
Anseremme
Hastière
Depth
0.5-1m
1-2m
0.5-1m
0.5-1m
1-2m
1-2m
1-2m
1-2m
1-2m
1-2m
Coordinates of the sampling site
Latitude, N
Longitude, E
50°27'1,37"
50°12'05,36"
50°08'42,26"
49°52'48,02"
50°26'6,97"
50°24'36,99"
50°21'4,40"
50°15'29,86"
50°14'26,45"
50°11'56,03"
4°51'44,28"
4°51'10,30"
4°49'52,65"
4°43'50,34"
4°51'53,94"
4°53'7,49"
4°52'19,55"
4°54'45,77"
4°53'58,72"
4°49'8,91"
Sampling date
May 2013, November
2013 and May 2014
21/09/2012
21/09/2012
22/09/2012
22/09/2012
22/09/2012
23/09/2012
ESM 2 Artificial substrate used to study the ecological links between Dreissena and other
macro-invertebrates in the Meuse River. Stainless steel cage during construction (a) and prior
to deployment (b); stainless steel cages attached to the concrete river bank at Monthermé at a
depth of 0.5-1m (c), to the sheet pile wall at Givet at a depth of 0.5-1m (d), to the concrete
wall of a fish ladder at Waulsort at a depth of 1-2m (e) and La Plante at a depth of 0.5-1m (f).
a
b
c
d
e
f
ESM 4 List of the 21 biological and ecological traits and their modalities of benthic freshwater macro-invertebrates proposed by Tachet et al.
(2010)
1
2
3
4
5
6
7
8
9
10
Traits
Maximal potential size
Life cycle duration
Potential number of cycles per year
Aquatic stages
Reproduction
Dispersal
Resistance forms
Respiration
Locomotion and substrate relation
Food
11
12
Feeding habits
13
14
15
Longitudinal distribution
Altitude
16
17
18
19
20
21
Current velocity (preferendum)
Trophic status (preferendum)
Salinity (preferendum)
Temperature
Saprobity
pH (preferendum)
Transversal distribution
Substrate (preferendum)
Modalities
≤ 0.25 cm; > 0.25-0.5 cm; > 0.5-1 cm; > 1-2 cm; > 2-4 cm; > 4-8 cm; > 8 cm
≤ 1 year; > 1 year
< 1; 1; > 1
egg; larva; nymph; adult
ovoviviparity; isolated eggs, free; isolated eggs, cemented; clutches, cemented or fixed ; clutches, free; clutches,
in vegetation; clutches, terrestrial; asexual reproduction
aquatic passive; aquatic active; aerial passive; aerial active
eggs, statoblasts; cocoons; housings against desiccation; diapause or dormancy; none
tegument; gill; plastron; spiracle; hydrostatic vesicle
flier; surface swimmer; full water swimmer; crawler; burrower; interstitial; temporarily attached; permanently
attached
microorganisms; detritus < 1mm; dead plant >= 1mm; living microphytes; living macrophytes; dead animal >=
1mm; living microinvertebrates; living macroinvertebrates; vertebrates
absorber; deposit feeder; shredder; scraper; filter-feeder; piercer; predator; parasite
river channel; banks, connected side-arms; ponds, pools, disconnected side-arms; marshes, peat bogs; temporary
waters; lakes; groundwaters
crenon; epirithron; metarithron; hyporithron; epipotamon; metapotamon; estuary; outside river system
lowlands; piedmont level; alpine level
flags/boulders/cobbles/pebbles; gravel; sand; silt; macrophytes; microphytes; twigs/roots; organic detritus/litter;
mud
null; slow; medium; fast
oligotrophic; mesotrophic; eutrophic
freshwater; brackish water
psychrophilic; thermophilic; eurythermic
xenosaprobic; oligosaprobic; b-mesosaprobic; a-mesosaprobic; polysaprobic
≤ 4; > 4-4.5; > 4.5-5; > 5-5.5; > 5.5-6; > 6
ESM 5 Number of Dreissena individuals observed in each artificial substrate collected in
May 2013, November 2013 and May 2014 in the Meuse River at 4 different localities
Station
Monthermé
Givet
Waulsort
La Plante
a
1
2524
0
0
May 13
b
2
2746
0
0
c
0
1851
0
0
November 13
a
b
c
0
2
0
2343
2076
1882
1
11
3
0
1
4
a
0
1974
5
0
May 14
b
1
2443
1
0
c
0
1409
16
1