Lance W. Noll
Kansas State University | Graduate Research Assistant
308 Coles Hall
Manhattan, KS 66502
785-532-4878
lwnoll@ksu.edu
OBJECTIVE
STEC CAP Objective 1: Detection
MAJOR PROFESSOR
Dr. T.G. Nagaraja
SHORT BIO
Lance Noll is from Greensburg, KS and has recently completed his M.S. degree
in Veterinary Biomedical Science. Lance is currently pursuing his Ph.D. degree
in Pathobiology at Kansas State under Dr. T.G. Nagaraja, and following his
schooling, plans to find a role in academia as a professor of microbiology.
Start Date: Fall 2012
Finish Date: Fall 2017
RESEARCH OBJECTIVES
A Fo u r - p l e x R e a l - t i m e P C R a s s a y, b a s e d o n r f b E , s t x 1 , s t x 2 ,
and eae genes, for the Detection and Q uantifica tion of
S h i g a t ox i n - p r o d u c i n g E s c h e r i c h i a c o l i O 1 5 7 i n C a t t l e F e c e s
Objective: Develop a multiplex, real-time quantitative PCR (mqPCR) assay for
the detection and quantification of E. coli O157 in cattle feces based on genes
that code for serogroup specific O157 antigen (rfbE O157) and three major
virulence factors, Shiga toxins 1 and 2 (stx1 and stx2) and intimin (eae).
Lance W. Noll
Evalua tion of chromID EHEC Agar for Detection of Seven
M a j o r S e r o g r o u p s o f S h i g a Tox i n - P r o d u c i n g E s c h e r i c h i a c o l i
(STEC) from Cattle Feces
Objective: To evaluate the performance of two chromogenic media, a chromID
EHEC (bioMérieux SA, Marcy-l'Etoile, France) and a MacConkey agar-based
medium with sucrose, sorbose, novobiocin and tellurite (modified Possé medium;
MP) for the detection of seven STEC in cattle feces.
Po o l i n g o f I m m u n o m a g n e t i c S e p a r a t i o n B e a d s D o e s N o t
Af fect Detection Sensitivity of Six Major Serogroups of
S h i g a Tox i n - P r o d u c i n g E s c h e r i c h i a c o l i ( S T E C ) i n C a t t l e
Feces
Objective: Determine whether pooling of IMS beads affect sensitivity of
detection of the six STEC in cattle feces and compare detection of STEC by
individual or pooled beads in fecal samples spiked with STEC or collected from
naturally-shedding cattle.
A Comparison of Culture- and PCR-Based Methods to Detect
S ev e n M a j o r S e r o g r o u p s o f S h i g a Tox i n - P r o d u c i n g
Escherichia coli (STEC) in Ca ttle Feces
Objective: Develop a culture-based method for the detection of six non-O157
STEC serogroups in cattle feces and compare this method, and the existing
culture method for detection of STEC O157, with PCR method for detection of
all seven STEC serogroups in cattle feces.
Concentra tion of Escherichia coli O157 in Ca ttle feces: An
Overview of Significance and Quantification Methods
Objective: Describe quantification methods for E. coli O157 in cattle feces,
including both traditional and emerging techniques, and report on available
enumeration data. Additionally, the role of super-shedders and their impact on
transmission of E. coli O157 into the environment and subsequent hide
contamination is discussed.
PUB L I CAT I O N S
Pending
A DDI T I O N A L AWA RDS / ACCO M PL I S HME N T S
2 0 1 3 Ka n s a s S t a t e U n i v e r s i t y P h i Z e t a Re s e a r c h D a y
Lance was the third place recipient in the Small Animal category at the annual Phi
Zeta Research competition held at Kansas State University for his oral
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Lance W. Noll
presentation entitled: “A Four Plex Real-Time PCR Assay for the Detection and
Quantification of Escherichia coli in Cattle Feces”.
2 0 1 3 A m e r i c a n A s s o c i a t i o n o f Ve t e r i n a r y L a b o r a t o r y
D i a g n o s t i c i a n s ( A AV L D ) C o n f e r e n c e
Lance was the recipient of the Richard L. Walker Award at the 2013 AAVLD
conference for his oral presentation entitled: “A Four Plex Real-Time PCR Assay
for the Detection and Quantification of Escherichia coli in Cattle Feces”.
2 0 1 3 G r a d u a t e S t u d e n t C o u n c i l Re s e a r c h a n d t h e S t a t e
Po s t e r C o m p e t i t i o n
Lance was the first place recipient in the Biological Sciences category at the
annual Graduate Student Council Research and the State poster competition held
at Kansas State University for his poster entitled: “A Four Plex Real-Time PCR
Assay for the Detection and Quantification of Escherichia coli in Cattle Feces”. In
addition to scholarship money, Lance was awarded the chance to travel to
Topeka to compete in the state-wide 2014 Capitol Graduate Research Summit.
2 0 1 3 C o n f e r e n c e o f Re s e a r c h Wo r k e r s i n A n i m a l D i s e a s e s
( C R WA D ) C o n f e r e n c e
Lance was the first place recipient in the Enteric Diseases category at the annual
CRWAD conference poster competition for his poster entitled: “A Four Plex RealTime PCR Assay for the Detection and Quantification of Escherichia coli in Cattle
Feces”.
2 0 1 4 C a p i t o l G r a d u a t e Re s e a r c h S u m m i t
Lance was the first place recipient at the 2014 Capitol Graduate Research
Summit for his poster entitled: “A Four Plex Real-Time PCR Assay for the Detection
and Quantification of Escherichia coli in Cattle Feces”. In addition to scholarship
money, Lance was awarded the opportunity to travel to Kansas City to attend the
2014 BioKansas One Health Summit.
2 0 1 4 C o n f e r e n c e o f Re s e a r c h Wo r k e r s i n A n i m a l D i s e a s e s
( C R WA D ) C o n f e r e n c e
Lance was the first place recipient in the Enteric Diseases category at the annual
CRWAD conference oral competition for his presentation entitled: “Pooling of
Immunomagnetic Separation Beads Does Not Affect Sensitivity of Detection of Six
Serogroups of Shiga Toxin-Producing Escherichia coli in Cattle Feces.”
2 0 1 5 Ka n s a s S t a t e U n i v e r s i t y P h i Z e t a Re s e a r c h D a y
Lance was the second place recipient in the Clinical and Applied Sciences
category at the annual Phi Zeta Research competition held at Kansas State
University for his oral presentation entitled: “A Four Plex Real-Time PCR Assay for
the Detection and Quantification of Escherichia coli in Cattle Feces”.
2 0 1 5 Ka n s a s S t a t e U n i v e r s i t y Re s e a r c h Fo r u m
Lance was the first place recipient in the Biological Sciences category at the
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Lance W. Noll
annual Kansas State University Research Forum for his oral presentation entitled:
“Culture- and PCR-based methods of detection and quantification of Shiga toxinproducing Escherichia coli O157 in cattle feces”.
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