Gene-to-Protein Service, E. coli Quotation Request Form Please complete the form and submit to order@proteinct.com. Our service representative will contact you shortly with a quote. CT-Express™ Gene-to-Protein service package specifications CT-Express™ Gene-to-Protein (CT-GOP) service package includes gene synthesis (codon optimized for E. coli), protein expression screening in E. coli, protein purification and delivery of >2mg purified protein (>75% purity) Membrane and toxic proteins are excluded. For expression in systems other than E. coli and customer special requests, please fill in and submit the Protein Expression and Purification Service Quotation Form instead. Customer provide Deliverables Turnaround time Charge • Gene Synthesis/subcloning • Expression screening • Purification optimization • Tag-removal Set up fee $1500 Protein sequence 6-8 weeks • QC by SDS PAGE • Full report Total $3000 • >4mg >75% pure protein *ProtenCT will design and use in house optimized contiditions and fusion tags for the project; small tags such as His tag will be left on protein of interest. *For non-membrane and non-toxic proteins, less than 100 kDa. A $1500 set-up fee pre-payment is required to start the project. If the protein cannot be expressed or purified per project description, we will send a project summary report and only the set-up fee will be collected. *Activity not guaranteed. For activity testing, additional charges apply. *Delivery time may vary depending on protein. Please contact your customer service scientist (info@proteinct.com) for more details. Customer Information If you have an existing account with ProteinCT, just fill in your name and email address or your Account No. Name of Requestor: Account No.: Name of PI: First: Last: Middle: Title: Organization: Phone: Fax: Email Address: Shipping Address Project Information Nature of Inquiry Pricing estimation Quote for ordering Estimated Project Initiation Immediately >6 months 1-3 months 3-6 months Other, please specify Research Use Only Clinical Applications Intended Use For grant application purpose Please note that we produce materials for research use only. We can perform developmental work for future clinical applications, but are not GMP-certified. Target Information Target: Protein name: Accession number: Species: Protein sequence: MW: Please indicate mutations/variants as compared to the sequence corresponding to the accession number. Target properties: Membrane-bound Secreted Cytosolic Protease Other enzymes IgG antibody Other features to consider, please specify: Nuclear Toxic to E.coli Periplasm