International Seminar 2013
Faculty of Agricultural Technology
No
1
2
Name
Jaya Mahar
Maligan,STP.,MP
Destination
Berlin
Germany
Prof.Dr.Ir. Simon B.
England
Widjanarko,M.App.Sc
Seminar
International
Conference of Food
Engineering and
Biotechnology 2013
17th Gums and
Stabilizers for The
Food Industry
Conference
Title
Production and
identification of
antimicrobacterial
compounds from
Microualgae Tetraselmis
chuii using ultrasound
assisted extraction
method
Optimization of
Ultrasound-assisted
extraction of Konjac Flour
From Amorphophallus
muelleri Blume
Schedule
22-23
May 2013
25-28
June 2013
Abstract
On the water ecosystem, microalgae were known as a natural producer of
energy and potential metabolites compounds. Marine microalgae produce
several bioactive compounds that potential to explore as functional food.
The functionality of extracellular microalgae products like as inhibitory and
trigger growth compounds. Tetraselmis chuii have bioactive component
such as fatty acids and esters is used to antibacterial agent. Antimicrobial
agent is a compound that can inhibit the growth of microbial. The extraction
process was affected by the extraction method, type of solvents, and
extraction condition. The advantage of ultrasound assisted extraction
method is to accelerate the extraction process. The type of solvents that
used in this research are influence the extract yield, especially for bioactive
compounds. Objectives of this study was gain more insight into the using of
different solvent and total cycle extraction to increase the yield of T. chuii
extract, to investigate the antimicrobial activity of T. chuii extract against
pathogenic microorganism, and identify the bioactive compounds of T. chuii
extract that potential as antimicrobial agents using GC-MS method. The
result showed that the highest yield extract was from acetone solvents
extraction (3 cycle), as much as 35.19 %. The bioactive compounds of T. chuii
extract has the potential as antimicrobial compounds against E. coli, S.
aureus, C .albicans and A. flavus. The bioactive compounds are present in T.
chuii extract was fatty acids, esters, alcohols, ketones, benzene, and alkanes.
Keywords—marine microalgae, Tetraselmis chuii, extract, antimicrobial
compound, ultrasound assisted extraction method
Konjac glucomannan is a water-soluble polysaccharide gum, which acts as a
soluble dietary fiber. Ultrasound-assisted extraction was used to extract
bioactive component from natural sources such as: xyloglucan from apple
pomace (Caili, et al., 2006) and polysaccharides from Zizyphus jujuba (Li et
al., 2007). Two factors central composite design (CCD) was used to
investigate the effect of multi-stage ethanol ultrasound-assisted extraction
condition namely extraction time (15-35 minutes) and Solvent/Flour ratio
(150-250 ml:25 g (v/w), on glucomannan, viscosity, calcium oxalate and
degree of whiteness of purified konjac flour (PKF). The second order
regression model with high R2 (≥ 0.80) value were significantly (p < 0.05)
fitted to predict the changes in glucomanan, viscosity and calcium oxalate of
PKF. Meanwhile, the first linier regression model with R2 (=0.5201) value
was significantly (p=0.0255) fitted to predict the changes in degree of
whiteness of PKF as function of multi-stage ethanol extraction variables. A
lack of fit component with p >0.05 showed significantly fitted to predict the
changes in glucomannan, viscosity, calcium oxalate and degree of whiteness
for explaining the physico-chemical properties of PKF. Thus ensuring
satisfactory fitness of the response surface model to the significant (p< 0.05)
independent variables. Predicted ultrasonically-assisted extraction process
for crude konjac flour (CKF) with multi-stage ethanol was extraction time
25.6 minutes and Solvent/Flour ratio was for 8.65:1 (ml/g). The response
surface methodology was succesfully employed to optimize purification
process conditions of PKF. Rechecking experiment conducted in two
duplicates, confirm the optimal conditions. The differences responses
between the predicted results and the real experiment were less than 5%.
Glucomannan, viscosity, and calcium oxalate were affected by combination
treatements of extraction time and S/F ratio, but the degree of whiteness
were predominantly influenced by extraction time (p < 0.05). This study can
be usefull to the development of industrial purification processes of CKF to
enhance the efficacy of a large scale purification processes.
Keywords: Konjac flour, Glucomannan, Ca-oxalate, Multi-stage Ethanol
extraction, ultrasonic.
3
4
Agustin Krisna
Wardani,Ph.D
Evi Kurniati,STP.,MT
Bangkok
Thailand
The 25th Annual
Meeting of The Thai
Society for
Biotechnology and
International
Conference
Exploration of newly
isolated bacteriophage
from beef tripe and
chicken intestine as
biosanotizing agent for
controlling biofilm
formation
16-19
Oktober
2013
Kyoto
Jepang
The 4th
International
Conference on
Sustainable Future
for Human Security
Potential use os
Aspergillus flavus strain
KRP1 in biodegradation of
mercury contaminant
19-21
Oktober
2013
5
6
Dr.Ir. Joni
Kusnadi,Msi
Wahyunanto Agung
Nugroho,STP.,M.Eng
Osaka
Jepang
The 2nd
International
Conference on Life
Science and
Biological
Engineering
Antibacterial activity
againts Escheria Coli and
Staphylococcus Aerus of
teak (Tectona Grandis)
leaves crude extract using
,icrowave assisted
extraction
7-9
November
2013
Bali
The International
Conference and
Workshop on
Chemical
Engineering UNPAR
2013
Effectiveness of the
addition of plant growth
promoting bacteria
(Azospirillum sp) in
increasing Chlorella sp
growth cultivated in tofu
processing wastewater
4-5
Desember
2013