Validation of a Metallomics Analysis of Placenta Tissue by InductivelyCoupled Plasma Mass Spectrometry Supporting Information A manuscript submitted to the journal Biological Trace Element Research James M. Harrington,1 Daniel J. Young,1 Rebecca J. Fry,2 Frank X. Weber,1 Susan S. Sumner,3 Keith E. Levine1* 1 Trace Inorganics Department, Technologies for Industry and the Environment, RTI International, Durham, NC27709 2 Gillings School of Global Public Health, University of North Carolina, Chapel Hill, NC 27599 3 Discovery Science Technology, RTI International, Research Triangle Park, NC, 27709, USA *Corresponding author e-mail: levine@rti.org Table S1. Nominal solvent and matrix standard and QC sample concentrations Name [A]a (ng/mL) (µg/g)c [B]b [Fe] [Zn] c c (ng/mL) (µg/g) (ng/mL) (µg/g) (ng/mL) (µg/g)c Matrix and solvent standard concentration levels STD-1d STD-2 STD-3 STD-4 STD-5 STD-6 STD-7 STD-8 STD-9 STD-10 0 0.0500 0.100 0.200 0.500 1.00 2.00 3.50 5.00 10.0 0 0.00214 0.00427 0.00855 0.0214 0.0427 0.0855 0.150 0.214 0.427 0 2.00 5.00 7.50 10.0 25.0 50.0 75.0 100 200 0 0.0855 0.214 0.321 0.427 1.07 2.14 3.21 4.27 8.55 0 500 750 2000 1500 2000 2500 3000 4000 5000 0 21.4 32.1 85.5 64.1 85.5 107 128 171 214 0 100 200 300 400 500 600 750 900 1000 0 4.27 8.55 12.8 17.1 21.4 25.6 32.1 38.5 42.7 Matrix QC levels; n = 6 replicates for level-1 and level-2; n = 3 replicates for all other levels. Level-1 0.0500 0.00214 2.00 0.0855 Level-2 0.100 0.00427 5.00 0.214 Level-3 0.200 0.00855 7.50 0.321 Level-4 0.500 0.0214 10.0 0.427 Level-5 1.00 0.0427 25.0 1.07 Level-6 3.50 0.150 75.0 3.21 Level-7 5.00 0.214 100 4.27 Level-8e 10.5 0.449 225 9.62 a Nominal concentration of As, Cd, Co, Cr, and Pb. b 500 750 1000 1500 2000 3000 5000 9000 21.4 32.1 42.7 64.1 85.5 128 214 385 100 200 300 400 500 750 1000 2250 4.27 8.55 12.8 17.1 21.4 32.1 42.7 96.2 Nominal concentration of Ni and Cu. c Calculated as: [Analyte] (ng/mL) x 10-mL digest volume x (569 g homogenate/0.64 g aliquot) x (1/208 g placenta) x (1 µg/1000 ng). d e Blank not included in matrix regression expressions. Dilution verification level. Table S2. Homogenate preparation Name Mass or volume Empty container + rod 95 g Empty container + rod + placenta tissue 303 g Placenta tissue 208 g Nitric acid addeda 297 mL Empty container + rod + placenta tissue (pre-heat) 707 g Empty container + rod + placenta tissue (post-heat) 664 g a Density of 70% (v/v) HNO3 = 1.42 g/mL; 421.74g acid added. Table S3. Significant polyatomic interferences for elements included in the analytical method validated here. Element, primary isotope 52 Cr (83.8%) 59 Co (100%) 75 As (100%) 56 Fe (91.7%) 63 Cu (69.2%) 66 Zn (%) 60 Ni (%) 208 Pb 111 Cd Interferences Ar12C, 40Ca12C, and 36Ar16O ArOH, CaOH, and ArNa 40 Ar35Cl 40 Ar16O, 40Ar16O, 39K17O 23 Na40Ar 26 Mg40Ar 44 Ca16O, 26Mg40Ar, No significant interferences No significant interferences 40