Validation of a Metallomics Analysis of Placenta Tissue by

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Validation of a Metallomics Analysis of Placenta Tissue by InductivelyCoupled Plasma Mass Spectrometry
Supporting Information
A manuscript submitted to the journal Biological Trace Element Research
James M. Harrington,1 Daniel J. Young,1 Rebecca J. Fry,2 Frank X. Weber,1 Susan S. Sumner,3
Keith E. Levine1*
1
Trace Inorganics Department, Technologies for Industry and the Environment, RTI
International, Durham, NC27709
2
Gillings School of Global Public Health, University of North Carolina, Chapel Hill, NC 27599
3
Discovery Science Technology, RTI International, Research Triangle Park, NC, 27709, USA
*Corresponding author e-mail: levine@rti.org
Table S1. Nominal solvent and matrix standard and QC sample concentrations
Name
[A]a
(ng/mL) (µg/g)c
[B]b
[Fe]
[Zn]
c
c
(ng/mL) (µg/g) (ng/mL) (µg/g) (ng/mL) (µg/g)c
Matrix and solvent standard concentration levels
STD-1d
STD-2
STD-3
STD-4
STD-5
STD-6
STD-7
STD-8
STD-9
STD-10
0
0.0500
0.100
0.200
0.500
1.00
2.00
3.50
5.00
10.0
0
0.00214
0.00427
0.00855
0.0214
0.0427
0.0855
0.150
0.214
0.427
0
2.00
5.00
7.50
10.0
25.0
50.0
75.0
100
200
0
0.0855
0.214
0.321
0.427
1.07
2.14
3.21
4.27
8.55
0
500
750
2000
1500
2000
2500
3000
4000
5000
0
21.4
32.1
85.5
64.1
85.5
107
128
171
214
0
100
200
300
400
500
600
750
900
1000
0
4.27
8.55
12.8
17.1
21.4
25.6
32.1
38.5
42.7
Matrix QC levels; n = 6 replicates for level-1 and level-2; n = 3 replicates for all other levels.
Level-1
0.0500 0.00214
2.00
0.0855
Level-2
0.100 0.00427
5.00
0.214
Level-3
0.200 0.00855
7.50
0.321
Level-4
0.500
0.0214
10.0
0.427
Level-5
1.00
0.0427
25.0
1.07
Level-6
3.50
0.150
75.0
3.21
Level-7
5.00
0.214
100
4.27
Level-8e
10.5
0.449
225
9.62
a
Nominal concentration of As, Cd, Co, Cr, and Pb.
b
500
750
1000
1500
2000
3000
5000
9000
21.4
32.1
42.7
64.1
85.5
128
214
385
100
200
300
400
500
750
1000
2250
4.27
8.55
12.8
17.1
21.4
32.1
42.7
96.2
Nominal concentration of Ni and Cu.
c
Calculated as: [Analyte] (ng/mL) x 10-mL digest volume x (569 g homogenate/0.64 g aliquot) x
(1/208 g placenta) x (1 µg/1000 ng).
d
e
Blank not included in matrix regression expressions.
Dilution verification level.
Table S2. Homogenate preparation
Name
Mass or volume
Empty container + rod
95 g
Empty container + rod + placenta tissue
303 g
Placenta tissue
208 g
Nitric acid addeda
297 mL
Empty container + rod + placenta tissue (pre-heat)
707 g
Empty container + rod + placenta tissue (post-heat)
664 g
a
Density of 70% (v/v) HNO3 = 1.42 g/mL; 421.74g acid added.
Table S3. Significant polyatomic interferences for elements included in the analytical method
validated here.
Element, primary isotope
52
Cr (83.8%)
59
Co (100%)
75
As (100%)
56
Fe (91.7%)
63
Cu (69.2%)
66
Zn (%)
60
Ni (%)
208
Pb
111
Cd
Interferences
Ar12C, 40Ca12C, and 36Ar16O
ArOH, CaOH, and ArNa
40
Ar35Cl
40
Ar16O, 40Ar16O, 39K17O
23
Na40Ar
26
Mg40Ar
44
Ca16O, 26Mg40Ar,
No significant interferences
No significant interferences
40
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