CV_Rugang Li - American Phytopathological Society

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CURRICULUM VITAE_RUGANG LI
USDA-ARS U.S. Vegetable Lab
2700 Savannah Highway
Charleston, SC 29414
Office (843) 402-5310; Home (613) 729-9056; Cell (843) 860-1282
Rugang.li@ars.usda.gov; Rugangli@yahoo.com
http://ars.usda.gov/pandp/people/people.htm?personid=46885
Career Objectives:
I am seeking a research or industry position where I can utilize my knowledge and skills
in molecular biology, plant pathology, plant virology, plant genetic engineering,
transgenic technology, molecular breeding, genomics, bioinformatics, and statistics to
better advance research efforts.
Highlighted Experience
1. More than 30 publications in peer-reviewed journals.
2. Molecular experience: PCR, RT-PCR, real-time RT-PCR, and isothermal
amplification, small RNA library construction, bioinformatics tools, statistics tools,
gene cloning and sequencing, northern hybridization, Southern hybridization,
genomic and cDNA library, etc.
3. Biological assays: growing fungi and bacteria on Petri dishes and viruses on plants,
inoculation of pathogens on plants, observance and recording plant symptoms.
4. Peer reviewer for journals: PLoS One, BMC Genetics, BMC Plant Biology, Fungal
Genetics and Biology, Planta, Journal of Virological Methods, and Crop Protection.
5. Professional communication skills in both written and verbal English;
6. Association: American Phytopathological Society (since 2002); American Society of
Microbiology (Since 2013).
Education
1. Ph.D. 1997, Plant molecular biology and genetic engineering, the Chinese Academy
of Agricultural Sciences, Beijing, China
2. M.Sc. 1988, Plant Pathology, China Agricultural University, Beijing, China.
3. B.Sc. 1985, Plant Protection, Shandong Agricultural University, Tai’an, China.
Professional Experience
2011.05 to present: Research plant pathologist
USDA-ARS, U.S. Vegetable Laboratory, Charleston, SC, U.S.A.
1. Key researcher of two USDA-NIFA SCRI projects: (1) A system-wide approach to
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manage plant and human pathogens on greenhouse tomatoes; (2) Understand the
global virus distribution in tomato and development of translational genomic tools to
accelerate tomato breeding for virus resistance;
Developed small RNA library protocol and generated 200 small RNA libraries from
various vegetable crops;
Identified 3 emerging tomato viruses and many known viruses;
Developed virus-specific real-time RT-PCR and isothermal amplification detection;
Designed a new PCR sequencing approach to rapidly obtain a viral genome sequence;
Developed a viroid cDNA expression system to generate infectious viroid RNAs;
Developed a protocol to evaluate the efficiency of disinfectants to viruses and viroids
Screening resistant wild tomatoes to PSTVd.
2004. 05 to 2010.07: Research scientist and project manager of Targeted Growth, Inc.
1. Managed soybean transformation project;
2. Studied the activity of seed-specific promoters in soybean embryos;
3. Improved the soybean transformation procedure;
4. Conducted 40 soybean transformation experiments;
5. Produced 750 transgenic soybean events;
6. Molecularly and genetically analyzed 250 fertile events;
7. Studied the resistance in transgenic lines to Sclerotinia in growth chamber;
8. A key member conducting field trials;
9. Prepared annual research report to TGI management team.
10. Represented TGI to attend scientific conferences.
2000. 11 to 2004. 04: NSERC Visiting Fellow at Agriculture and Agri-Food Canada,
Saskatoon Research Center.
1. Constructed two Sclerotinia sclerotiorum cDNA libraries;
2. Produced more than 2000 expressed sequence tags (ESTs);
3. Identified 774 S. sclerotiorum unique gene;
4. Cloned 4 endo-polygalacturonases genes and 2 exo-polygalacturonases genes;
5. Expressed 4 endo-polygalacturonases with Pichia pastoris expression system and
evaluated product activities in vitro;
6. Constructed Brassica napus DH12075 genomic library and bacterial artificial
chromosome (BAC) library;
7. Identified and characterized 16 polygalacturonase inhibitor (PGIP) genes from above
B. napus DH12075 libraries;
8. Established a rapid resistance-screening method in B. napus to S. sclerotiorum.
1999. 01 to 2000. 10: Postdoctoral Research Associate, Department of Plant Pathology,
University of Arkansas, Fayetteville, AR, USA.
1. Developed a convenient Agrobacterium-mediated transient expression assay for rapid
analysis of plant promoters and transcription factors in vivo;
2. Produced more than 100 transgenic tobacco lines expressing tobacco transcription
factor myb1 gene;
3. Biologically and molecularly characterized virus resistance mediated by myb1 gene in
those transgenic lines.
1998. 03 to 1998. 12: Visiting scientist, USDA-ARS, Crop Protection and Management
Research Laboratory, University of Georgia, Tifton, GA, USA.
1. Revealed the genetic variation within the maize population GT-MAS:gk and the
relationship between genetic variation and resistance to Aspergillus flavus;
2. Identified restriction fragment length polymorphism (RFLP) markers associated with
silk maysin, antibiosis to corn earworm (Lepidoptera: Noctuidae) larvae, in a dent and
sweet corn cross.
1996. 10 to 1997. 03: Visiting Scientist, Department of Molecular Biology, Centre for
Plant Breeding and Reproduction Research, DLO, Wageningen, Holland.
1. Produced 55 potato transgenic events;
2. Molecularly characterized those events;
3. Evaluate the disease resistance in transgenic plants to Phytophthora infestans
infection in greenhouse.
1996. 09: Attended the “Training course of Bioinformatics” at the International Center of
Genetic Engineering and Biotechnology, Trieste, Italy.
1994.03 to 1998.02: Research group leader in the Biotechnology Research Center,
Chinese Academy of Agricultural Sciences, Beijing, China.
1. Developed a research project to study the resistance in both potato and tomato to
Phytophthora infestans;
2. Developed DNA marker technology to study the genetic diversity in oilseed Brassica
species, taro and apple;
3. Developed the RT-PCR detection for sweet potato feathery mottle virus (SPFMV)
and sweet potato latent virus (SPLV);
4. Collaborated with the International Potato Center (CIP), the International Center of
Genetic Engineering and Biotechnology (ICGEB), and the International Plant Genetic
Resources Institute (IPGRI).
1992. 09 to 1994. 02: Visiting Fellow with the USDA-ARS, Plant Genetic Resources
Unit, Cornell University, Geneva, NY, USA.
1. Utilized PCR-based marker technology to study the genetic diversity in Vetiver grass,
vegetable Brassica species, and apple;
2. Identified a DNA marker closely linked to pea seed-born mosaic virus.
1988.07 to 1992.09 Research Associate in the Biotechnology Research Center, Chinese
Academy of Agricultural Sciences, Beijing, China.
1. Survey of sweet potato viral disease in China
2. Established several hybridoma cell lines secreting monoclonal antibody specific to
Potato virus X (HB strain), Sweet potato feather mottle viruse, and Pseudomonas
solanacearum;
3. Developed antibody-based detection assays (DAS-ELISA, Dot-blot ELISA, Biotin
ELISA) for viruses and bacteria infecting sweet potato and potatoes.
Awards and Honors
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2012-2013: USDA-ARS Outstanding performance award.
2011-2012: USDA-ARS Superior performance award.
2000: Chinese Ministry of Education Award for distinguished Ph.D. thesis in China.
1998: USDA-ARS Appreciation Award for "Molecular genetics study and the
development of DNA marker linked to earworm resistance in corn".
5. 1993: World Bank Award for "Application of molecular methods and statistical
analyses for discrimination of accessions and clones of vetiver grass".
Oral presentations
1. Molecular characterization and detection of Mexican papita viroid. The American
Phytopathological Society (APS) and the Mycological Society of America (MSA) –
Joint Meeting. August 9-15, 2013, Austin, TX, USA
2. Mexican papita viroid: Molecular and biological characterization. 27th Tomato
Disease Workshop. October 16-18, 2012. Wooster, Ohio, USA
3. Characterization and detection of Tomato necrotic stunt virus, a novel potyvirus
infecting greenhouse tomatoes in Mexico. APS annual meeting. August 4-8, 2012,
Providence, RI, USA
4. Overexpression of the Arabidopsis transcription factor REVOLUTA leads to
increased soybean seed yield in replicated field trials. World Soybean Research VIII.
August 10-15, 2009, Beijing, China
5. Overexpression of the Arabidopsis Transcription factor REVOLUTA leads to
increased soybean seed size and yield. 2008 World Congress on In Vitro Biology.
June 24-18, 2008, Tucson, Arizona, USA
6. Salicylic acid-induced transcription factor NtMYB1 regulates N gene-mediated virus
resistance. 41st Annual Plant Development Workshop, November 20, 2007. Carleton
University, Ottawa, Canada
7. Genomics of Sclerotinia sclerotiorum: expressed sequence tag analysis reveals genes
associated with fungal pathogenesis. AAFC Fifth Annual Genomics Meeting. August
21-23, 2003, Saskatoon, SK, Canada.
8. Polygalacturonases of Sclerotinia sclerotiorum: expression during saprophytic and
parasitic modes of growth. APS annual meeting, July27-31, 2002, Milwaukee, USA
Students supervised
Chen Rumei: Ph.D. student in plant pathology
Department of Plant Pathology, China Agricultural University, Beijing, China.
Thesis: Analysis of transgenic potato expressing dual PR proteins (PR1a and PR5) for
their resistance to Phytophthora infestans infection.
Shen Yuhuan: M.Sc. student in plant molecular biology
The Graduate School of Chinese Academy of Agricultural Sciences, Beijing, China.
Thesis: Cloning of sweet potato feathery mottle virus and sweet potato latent virus coat
protein genes and development of RT-PCR detection technologies.
Other individuals supervised
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Alan Wilder
Andrea Gilliard
Anna Butron
Shuiqing Zhao
5. Ningfeng Wu
6. Guangchun Shi
7. Maolin Li
8. Yingying Sun
9. Li Zhu
10. Xiaoming Wu
11. Zhenglin Ju
12. Xiaomei Zhu
13. Aihong Xue
small RNA library construction
small RNA library construction
DNA isolation and Southern hybridization
PCR, RT-PCR, RNA isolation, mRNA purification, cDNA
synthesis, and cDNA library construction
Potato transformation
Molecular cloning and PCR
PCR-based DNA marker
Potato transformation and virus detection
PCR-based DNA marker
PCR-based DNA marker
Antibody purification and enzyme-labeling
Antibody purification and enzyme-labeling
Virus isolation and serological detection
Publications in peer-reviewed journals
1. Li R, Ling K-S (2014) Development of RT-LAMP assay for rapid detection of an
emerging potyvirus: tomato necrotic stunt virus. Journal of Virological Methods 200:
35-40
2. Ling K-S, Li R, Groth-Helms D, Assis-Filho F (2014) First report of potato spindle
tuber viroid naturally infecting field tomatoes in the Dominican Republic. Plant
Disease 98:701
3. Ling K-S, Li R, Zhang W (2014) First report of cucumber green mottle mosaic virus
infecting greenhouse cucumber in Canada. Plant Disease 98:701
4. Li R, Fei Z, Ling K-S (2014) Molecular and biological properties of Tomato necrotic
stunt virus and development of a sensitive real-time RT-PCR assay. Archives of
Virology 159:353-358.
5. Li R, Gao S, Fei Z, Ling K-S (2013) Complete genome sequence of a new
tobamovirus naturally infecting tomatoes in Mexico. Genome Announc. 1:e00794-13.
6. Ling K-S, Li R, Blesdsoe M (2013) Pepino mosaic virus genotype shifting in North
America and development of specific RT-LAMP for rapid genotype identification.
Virology Journal 10:117.
7. Ling K-S, Li R, Panthee DR, Gardner RG (2013) First report of potato spindle tuber
viroid naturally infecting greenhouse tomatoes in North Carolina. Plant Disease
97:148
8. Li R, Gao S, Hernandez AG, Wechter WP, Fei Z, Ling K-S (2012) Deep Sequencing
of Small RNAs in Tomato for Virus and Viroid Identification and Strain
Differentiation. PLoS ONE 7:e37127
9. Li R, Bancroft B, Zhao SQ, Zheng SQ, Blahut-Beatty L, De Rocher J, Simmonds D
(2011) Multiple inserts of gene of interest and selection marker gene are co-integrated
and stably transmitted as a single genetic locus in transgenic soybean plants. In Vitro
Cellular and Developmental Biology-Plant 47:274-281
10. Hegedus DD, Li R, Buchwaldt L, Parkin I, Whitwill S, Coutu C, Bekkaoui D,
Rimmer S. R. (2008) Brassica napus possesses an expanded set of polygalacturonase
inhibitor protein genes that are differentially regulated in response to Sclerotinia
sclerotiorum infection, wounding and defense hormone treatment. Planta 228:241–
253
11. Li R, Rimmer R, Buchwaldt L, Sharpe AG, Seguin-Swartz G, Hegedus DD (2004)
Interaction of Sclerotinia sclerotiorum with a resistant Brassica napus cultivar:
expressed sequence tag analysis identifies genes associated with fungal pathogenesis.
Fungal Genetics and Biology 41:735-753
12. Li R, Rimmer R, Buchwaldt L, Sharpe AG, Seguin-Swartz G, Hegedus DD (2004)
Interaction of Sclerotinia sclerotiorum with Brassica napus: Cloning and
characterization of endo- and exo-polygalacturonases expressed during saprophytic
and parasitic modes. Fungal Genetics and Biology 41:754-765
13. Li R, Rimmer R, Yu M, Sharpe A, Hegedus DD (2003) Two Brassica napus PGIP
genes are expressed at different levels in response to biotic and abiotic stresses. Planta
217:299-308
14. Butron A, Li R, Guo BZ, Widstrom NW, Snook ME, Cleveland TE, Lynch RE
(2001) Molecular markers to increase corn earworm resistance in a maize population.
Maydica 46:117-124
15. Guo BZ, Li R, Widstrom NW, Lynch RE, Cleveland TE (2001) Genetic variation
within the maize population GT-MAS:gk and its relationship with resistance to
Aspergillus flavus. Theor. Appl. Genet. 103:533-539
16. Guo BZ, Zhang ZJ, Li R, Widstrom NW, Snook ME, Lynch RE, Plaisted D (2001)
RFLP markers associated with silk Maysin, antibiosis to corn earworm larvae
(Lepidoptera: Noctuidae), in a dent and sweet corn cross. J. Economic Entomology
94:564-571
17. Yang Y, Li R, Qi M (2000) In vivo analysis of plant promoters and transcription
factors by agroinfiltration of tobacco leaves. Plant Journal 22:543-551
18. Li R, Wu NF, Fan YL, Song BF (1999) Transgenic potato plants expressing osmotin
gene inhibit fungal development in inoculated leaves. Chinese Journal of
Biotechnology 15:71-75
19. Li R, Fan YL (1999) Reduction of lesion growth rate of late blight plant disease in
transgenic potato plants expressing harpin protein. Science in China (Series C) 42:96101.
20. Wu XM, Wu NF, Qian XZ, Li R, Huang FH, Zhu L (1998) Phenotypic and genotypic
changes in rapeseed after 18 years of storage and regeneration. Seed Science
Research 8 (Suppl. 1):55-64.
21. Zhu L, Li R, Wu XM, Wu NF, Qian XZ, Fan YL (1998) RAPD analysis of genetic
diversity among partial Chinese accessions of Brassica rapa. Chinese Biodiveristy
6(2):99-104
22. Wu NF, Li R, Wu XM, Zhu L, Fan YL, Qian XZ (1997) RAPD molecular markers
and genetic diversity among 40 accessions of Brassica napus. Chinese Biodiversity
5:246-250
23. Ran JP, McFerson JR, Li R, Kresovich S, Lamboy WF (1995) Identities and
relationships among Chinese vegetables Brassicas as determined by random
amplified polymorphic DNA markers. J. Amer. Soc. Hort. Sci. 120:548-555
24. Kresovich S, Lamboy WF, Li R, Ren JP, Szewc-McFadden AK, Bliek SM (1994)
Application of molecular methods and statistical analysis for discrimination of
accessions and clones of vetiver grass. Crop Science 34:805-809
25. Li R, Cai SH, Liu J, Feng LX, Hsu H-T (1993) Monoclonal antibodies reacted
specifically to potato virus X - HB strain (PVX-HB). Acta Phytopathologica Sinica
23:5-9
26. Li R, Guo J, Ju ZL, Zhu XM, Liang H, Wang YC (1992) Monoclonal antibodies
against potato virus X and Y and their use in virus detection. Chinese Journal of
Potato 6:233-236
27. Li R, Chiu WF (1992) Ultrastructural observation on Dahlia leaf tissue infected with
dahlia mosaic virus. Acta Virologica Sinica 7:459-462
28. Li R, Xue A, Zhu XM, Cai SH (1992) Construction of hybridomas secreting
monoclonal antibodies against sweet potato feathery mottle virus and use of antibody
for detection of SPFMV. Chinese Journal of Biotechnology 8:277-281
29. Qiu BS, Zhao F, Wang XF Tian P, Li R, Cai S. (1992) Molecular cloning and
application of cDNA probe of sweet potato feathery mottle virus. Acta
Microbiologica Sinica 32:242-246
30. Li R, Zhu XM, Xue AH, Cai SH, Wang XF (1992) Identification of Langfang isolate
of sweet potato feathery mottle virus. Acta Phytopathologica Sinica 22:319-322
31. Li R, Cai SH, Salazar LF (1990) Serological detection of viruses on sweet potato in
China. Acta Phytopathologica Sinica 22:189-194
32. Li R, Chiu WF (1990) Three viruses associated with mosaic symptoms on Dahlia
pinnata Cav in Beijing. Acta Phytopathologica Sinica 20:111-116
Literature Reviews
1. Li R, Fan YL (2000) Advances on genetic engineering of plant resistance against
mycological diseases. Progress in Biotechnology 2:9-13.
2. Li R, Wu NF, Fan YL, Song BF (1997) Advances on resistance in potato against late
blight disease. Chinese Journal of Potato 11:243-250
3.
Li R (1995) Application of molecular diagnostics to plant genetic resources
conservation. Chinese Biodiversity 3 (suppl.):22-29
4. Li R (1994) Molecular aspects of plant-pathogen interactions and strategies of
disease control. Chinese Journal of Agricultural Biotechnology, 2:1-13
Proceedings, newsletters and bulletins
1. Li ML, Li R, Zhou MD, Eyzaguirre P, and Ayad WG (2000) Analysis of correlation
between ethnobotany and genetic diversity of taro in China using RAPD assay. In:
Proceedings of the Twelfth Symposium of the International Society for Tropical Root
Crops (Eds: M. Nakatani and K. Komaki), P544-546. Tsukuba, Japan, 10-16
September 2000
2. Guo BZ, Butron A Li R, Zhang ZJ, Widstron NW, McMullen MD, Lynch RE (2000)
Identification of a gene at the syntenic sh2-a1 region in maize. Maize Genetics
Newsletter 74:64-66
3. Li R, Zhu L, Wu NF, Fan YL, Wu XM and Qian XZ (1997) Genetic variation and
relatedness among oilseed accessions of Brassica juncea Czern. et Cross. Chinese
Biotechnology Bulletin 5:26-31
4. Li R, McFerson JR and Kresovich S (1997) Genetic variation of acetohydroxyacide
synthetase gene among cultivated Brassica species and its use in discriminating
accessions of Brassica napus L. Plant Genetic Resources Newsletter 111:59-63
5. Li R, Wang YC, Cai SH, Zhang XD, Xie DX and Fan YL (1992) Monoclonal
antibodies for detection bacteriocin M2 from strain M2 of Pseudomonas
solanacearum. Chinese Science Bulletin 37:56-60
6. Li R, Xue AH, Zhu XM, and Cai SH (1992) Monoclonal antibodies to sweet potato
feathery mottle virus. In: Agricultural Biotechnology. Proceedings of Asia-Pacific
Conference on Agricultural Biotechnology, August 20-24, 1992, Beijing, China.
China Science and Technology Publishing House, p.593-594
7. Li R, Cai SH, Liu J, Feng LX, and Hsu H-T (1990) The establishment of hybridomas
secreting specific antibodies against HB strain of potato virus X by using mice with
induced immunological tolerance to other strains of PVX. In: Potato and Sweet
Potato Research in China, CAAS and CIP Region VIII. p. 51-56
Meeting Abstracts and Posters
1. Li R, Ling K-S. Screening tomato germplasm for resistance to Potato spindle tuber
viroid. 2014 Tomato Breeder’s Round Table. Sepetmebr 14-17, 2014, Mills River,
NC
2. Li R, Berendsen S, Fei Z, Ling K-S. Discovery of a new genotype of Squash mosaic
virus through deep sequencing of small RNAs and development of a qRT-PCR for
broad spectrum detection. APS-CPS Joint Meeting, August 9-13, 2014. Minneapolis,
MN
3. Li R, Ling K-S (2013) Molecular characterization and detection of Mexican papita
viroid. Phytopathology 103(Suppl. 2):S2.80
4. Ling K-S, Li R, Fei Z (2013) Global virus survey in tomato using small RNA deep
sequencing technology. Acta phytopathologica sinica 43 (suppl.). 281-282
5. Ling K-S, Li R, Sombat S (2013) Characterization and detection of emerging viroids
in North American greenhouse tomatoes. International workshop on viroids and
satellite RNAs (IWVds). p. 5
6. Ling K-S, Li R, Bledsoe M (2013) Pepino mosaic virus genotype shift in North
America and rapid genotype identification using loop-mediated isothermal
amplification. Acta phytopathologica sinica 43 (suppl.). p. 402
7. Li R, Baysal-Gurel F, Kurowski C, Miller SA, Ling K (2013) Evaluation of
disinfectants to control mechanical transmission of tomato viruses and viroids in
greenhouse tomatoes. The 4th International Symposium on Tomato Diseases and 28th
U.S. Annual Tomato Disease Workshop. p 30
8. Baysal-Gurel F, Li R, Ling K, Kurowski C, Miller SA (2013) Developing hygiene
protocols against mechanically transmitted pathogens in greenhouse tomato
production systems. The 4th International Symposium on Tomato Diseases and 28th
U.S. Annual Tomato Disease Workshop. p 29
9. Ling K, Li R, Gao S, Fei Z (2013) Understanding the global distribution of tomato
viruses and viroids using next-generation sequencing technology. The 4th
International Symposium on Tomato Diseases and 28th U.S. Annual Tomato Disease
Workshop. p 24
10. Li R, Ling K-S (2012) Molecular and biological characterization of Mexican papita
viroid. 27th Annual Tomato Disease Workshop, P28
11. Baysal-Gurel F, Li R, Henderson DR, Ling K-S Miller SA (2012) Sensitivity of
mechanically transmitted pathogens to different disinfectants. 27th Annual Tomato
Disease Workshop, P29
12. Ling K-S, Li R, Panthee DR, Gardner RG (2012) Identification of Potato spindle
tuber viroid naturally infecting greenhouse tomatoes in North Carolina and its
potential in seed transmission. 27th Annual Tomato Disease Workshop, P27
13. Li R, Fei Z, Ling K (2012) Characterization and detection of Tomato necrotic stunt
virus, a novel potyvirus infecting greenhouse tomatoes in Mexico. Phytopathology
102:S4.70
14. Ling K, Li R (2012) Genetic diversity and phylogenetic analysis revealed Pepino
Mosaic Virus in North-America has shifted to a more aggressive CH2 genotype.
Phytopathology 102:S4.71
15. Ling K, Li R, Wechter WP, Hernamdez AG, Fei Z (2012) Using small RNA
technology to efficiently identify tomato viruses and viroids in mixed-infected field
aamples. 26th Annual Tomato Disease Workshop. p 22
16.
Li R, Bancroft B, Zhao SQ, Zheng SQ, Blahut-Beatty L, De Rocher J, Simmonds D
(2010) Multiple inserts of gene of interest and selection marker gene are co-integrated
and stably transmitted as a single genetic locus in transgenic soybean plants. 12th
World Congress of the International Association for Plant Biotechnology, June 6-11,
2010, St. Louis, Missouri, USA
17. Li R, Bancroft B, Lum K, Nguyen T, De Rocher J, Simmonds D (2008)
Overexpression of the Arabidopsis Transcription Factor REVOLUTA Leads to
Increased Soybean Seed Size and Yield. In vitro Cellular and Developmental
Biology, Volume 44, Issue Abstract, P-1012
18. Buchwaldt L, Li R, Hegedus DD, Rimmer SR (2005) Pathogenesis of Sclerotinia
sclerotiorum in relation to screening for resistance. The XIIIth International
Sclerotinia Workshop. 12-16 June 2005, Monterey, California, USA
19. Hegedus DD, Rimmer R, Buchwaldt L, Li R, Sharpe A (2005) Defining the
biochemical mechanisms underlying resistance to stem rot disease (Sclerotinia
sclerotiorum) in Brassica napus. The XIIIth International Sclerotinia Workshop. 1216 June 2005, Monterey, California, USA
20. Buchwaldt L, Hegedus D, Li R, Lydiate D, Rimmer R (2005) Preliminary mapping
of physiological resistance to Sclerotinia sclerotiorum in Brassica napus. The XIIIth
International Sclerotinia Workshop. 12-16 June 2005, Monterey, California, USA
21. Hegedus D, Rimmer R, Buchwaldt L, Li R, Sharpe A (2004) Gene expression
associated with Sclerotinia infection and resistance in Brassica napus. 2nd Canadian
Plant Genomics Workshop. August 29 - September 1, 2004, Québec City, Canada
22. Li R, Rimmer R, Sharpe A, Seguin-Swartz G, Hegedus D (2002) Polygalacturonases
of Sclerotinia sclerotiorum: expression during saprophytic and parasitic modes of
growth. Phytopathology 92:S47. Publication no. P-2002-0338-AMA
23. Li R, Rimmer R, Yu M, Gruber M, Sharpe A, Seguin-Swartz G, Hegedus D (2002)
Brassica napus polygalacturonase inhibitor genes are differentially regulated by
wounding and fungal infection. Phytopathology 92:S47. Publication no. P-20020339-AMA
24. Li R, Klessig DF, Yang Y (2000) Overexpression of a salicylic acid-inducible myb
gene in transgenic tobacco enhances the N gene-mediated resistance to TMV.
Phytopathology 90:S46. Publication no. P-2000-0330-AMA
25. Yang Y, Li R, Lee M (1999) Involvement of plant myb genes in disease resistance
and susceptibility. 9th International Congress of Molecular Plant-Microbe
Interactions, July 25-30, 1999, Amsterdam, the Netherlands
26. Guo BZ, Li R, Widstrom NW, Snook ME, McMullen MD, Lynch RE (1999)
Preliminary identification and sequences of a gene in the sh2-a1 region that affects
silk maysin concentration in maize silks. 41st Annual Maize Genetics Conference,
March, 1999, Wisconsin, USA
27. Li R, Guo BZ, Widstrom NW, Lynch RE, Cleveland TE (1998) Genetic variation in
maize germplasm GT-MAS:gk, as revealed by RAPD-PCR markers and the
relationship to resistance to Aspergillus flavus. Phytopathology 88:S53. Publication
no. P-1998-0384-AMA
28. Li R, Guo BZ, Widstrom NW, Lynch RE, Cleveland TE (1998) Genetic variation in
GT-MAS:gk population and possible PCR and RFLP markers linked to resistance to
Aspergillus flavus. Aflatoxin Elimination Workshop. Oct. 25-27, 1998, St. Louis,
MO, USA
29. Guo BZ, Widstrom NW, Li R, Cong P, Lynch RE, Snook ME, Wilson DM,
Cleveland TE (1998) Pyramiding resistance to Aspergillus flavus and corn earworm
to control preharvest aflatoxin contamination: The possibility with genetic mapping
and DNA markers linked to resistance traits. Aflatoxin Elimination Workshop. Oct.
25-27, 1998, St. Louis, MO, USA
30. Guo BZ, Li R, Cong P, Widstrom NW, Lynch RE, Wilson DM, Cleveland TE (1998)
RFLP screening to map corn resistance to corn earworm and Aspergillus flavus.
Aflatoxin Elimination Workshop. Oct. 25-27, 1998, St. Louis, MO, USA
31. Guo BZ, Li R, Cong P, Widstrom NW, Wiseman BR, Snook ME, Lynch RE (1998)
Characterization of a chromosome region which enhance maysin production in silks
and reduces corn growth and earn damage. Aflatoxin Elimination Workshop. Oct. 2527, 1998, St. Louis, MO, USA
Book Translation
1. Li R and Zhang CY (1999) Methods in Plant Molecular biology and Biotechnology
(Authors: Glick B. R. and Thompson J. E., published in 1993 by CRC Press, Inc.,
Boca Raton, Florida, U.S.A.), Chongqing Publishing House, Chongqing, China
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