CURRICULUM VITAE_RUGANG LI USDA-ARS U.S. Vegetable Lab 2700 Savannah Highway Charleston, SC 29414 Office (843) 402-5310; Home (613) 729-9056; Cell (843) 860-1282 Rugang.li@ars.usda.gov; Rugangli@yahoo.com http://ars.usda.gov/pandp/people/people.htm?personid=46885 Career Objectives: I am seeking a research or industry position where I can utilize my knowledge and skills in molecular biology, plant pathology, plant virology, plant genetic engineering, transgenic technology, molecular breeding, genomics, bioinformatics, and statistics to better advance research efforts. Highlighted Experience 1. More than 30 publications in peer-reviewed journals. 2. Molecular experience: PCR, RT-PCR, real-time RT-PCR, and isothermal amplification, small RNA library construction, bioinformatics tools, statistics tools, gene cloning and sequencing, northern hybridization, Southern hybridization, genomic and cDNA library, etc. 3. Biological assays: growing fungi and bacteria on Petri dishes and viruses on plants, inoculation of pathogens on plants, observance and recording plant symptoms. 4. Peer reviewer for journals: PLoS One, BMC Genetics, BMC Plant Biology, Fungal Genetics and Biology, Planta, Journal of Virological Methods, and Crop Protection. 5. Professional communication skills in both written and verbal English; 6. Association: American Phytopathological Society (since 2002); American Society of Microbiology (Since 2013). Education 1. Ph.D. 1997, Plant molecular biology and genetic engineering, the Chinese Academy of Agricultural Sciences, Beijing, China 2. M.Sc. 1988, Plant Pathology, China Agricultural University, Beijing, China. 3. B.Sc. 1985, Plant Protection, Shandong Agricultural University, Tai’an, China. Professional Experience 2011.05 to present: Research plant pathologist USDA-ARS, U.S. Vegetable Laboratory, Charleston, SC, U.S.A. 1. Key researcher of two USDA-NIFA SCRI projects: (1) A system-wide approach to 2. 3. 4. 5. 6. 7. 8. manage plant and human pathogens on greenhouse tomatoes; (2) Understand the global virus distribution in tomato and development of translational genomic tools to accelerate tomato breeding for virus resistance; Developed small RNA library protocol and generated 200 small RNA libraries from various vegetable crops; Identified 3 emerging tomato viruses and many known viruses; Developed virus-specific real-time RT-PCR and isothermal amplification detection; Designed a new PCR sequencing approach to rapidly obtain a viral genome sequence; Developed a viroid cDNA expression system to generate infectious viroid RNAs; Developed a protocol to evaluate the efficiency of disinfectants to viruses and viroids Screening resistant wild tomatoes to PSTVd. 2004. 05 to 2010.07: Research scientist and project manager of Targeted Growth, Inc. 1. Managed soybean transformation project; 2. Studied the activity of seed-specific promoters in soybean embryos; 3. Improved the soybean transformation procedure; 4. Conducted 40 soybean transformation experiments; 5. Produced 750 transgenic soybean events; 6. Molecularly and genetically analyzed 250 fertile events; 7. Studied the resistance in transgenic lines to Sclerotinia in growth chamber; 8. A key member conducting field trials; 9. Prepared annual research report to TGI management team. 10. Represented TGI to attend scientific conferences. 2000. 11 to 2004. 04: NSERC Visiting Fellow at Agriculture and Agri-Food Canada, Saskatoon Research Center. 1. Constructed two Sclerotinia sclerotiorum cDNA libraries; 2. Produced more than 2000 expressed sequence tags (ESTs); 3. Identified 774 S. sclerotiorum unique gene; 4. Cloned 4 endo-polygalacturonases genes and 2 exo-polygalacturonases genes; 5. Expressed 4 endo-polygalacturonases with Pichia pastoris expression system and evaluated product activities in vitro; 6. Constructed Brassica napus DH12075 genomic library and bacterial artificial chromosome (BAC) library; 7. Identified and characterized 16 polygalacturonase inhibitor (PGIP) genes from above B. napus DH12075 libraries; 8. Established a rapid resistance-screening method in B. napus to S. sclerotiorum. 1999. 01 to 2000. 10: Postdoctoral Research Associate, Department of Plant Pathology, University of Arkansas, Fayetteville, AR, USA. 1. Developed a convenient Agrobacterium-mediated transient expression assay for rapid analysis of plant promoters and transcription factors in vivo; 2. Produced more than 100 transgenic tobacco lines expressing tobacco transcription factor myb1 gene; 3. Biologically and molecularly characterized virus resistance mediated by myb1 gene in those transgenic lines. 1998. 03 to 1998. 12: Visiting scientist, USDA-ARS, Crop Protection and Management Research Laboratory, University of Georgia, Tifton, GA, USA. 1. Revealed the genetic variation within the maize population GT-MAS:gk and the relationship between genetic variation and resistance to Aspergillus flavus; 2. Identified restriction fragment length polymorphism (RFLP) markers associated with silk maysin, antibiosis to corn earworm (Lepidoptera: Noctuidae) larvae, in a dent and sweet corn cross. 1996. 10 to 1997. 03: Visiting Scientist, Department of Molecular Biology, Centre for Plant Breeding and Reproduction Research, DLO, Wageningen, Holland. 1. Produced 55 potato transgenic events; 2. Molecularly characterized those events; 3. Evaluate the disease resistance in transgenic plants to Phytophthora infestans infection in greenhouse. 1996. 09: Attended the “Training course of Bioinformatics” at the International Center of Genetic Engineering and Biotechnology, Trieste, Italy. 1994.03 to 1998.02: Research group leader in the Biotechnology Research Center, Chinese Academy of Agricultural Sciences, Beijing, China. 1. Developed a research project to study the resistance in both potato and tomato to Phytophthora infestans; 2. Developed DNA marker technology to study the genetic diversity in oilseed Brassica species, taro and apple; 3. Developed the RT-PCR detection for sweet potato feathery mottle virus (SPFMV) and sweet potato latent virus (SPLV); 4. Collaborated with the International Potato Center (CIP), the International Center of Genetic Engineering and Biotechnology (ICGEB), and the International Plant Genetic Resources Institute (IPGRI). 1992. 09 to 1994. 02: Visiting Fellow with the USDA-ARS, Plant Genetic Resources Unit, Cornell University, Geneva, NY, USA. 1. Utilized PCR-based marker technology to study the genetic diversity in Vetiver grass, vegetable Brassica species, and apple; 2. Identified a DNA marker closely linked to pea seed-born mosaic virus. 1988.07 to 1992.09 Research Associate in the Biotechnology Research Center, Chinese Academy of Agricultural Sciences, Beijing, China. 1. Survey of sweet potato viral disease in China 2. Established several hybridoma cell lines secreting monoclonal antibody specific to Potato virus X (HB strain), Sweet potato feather mottle viruse, and Pseudomonas solanacearum; 3. Developed antibody-based detection assays (DAS-ELISA, Dot-blot ELISA, Biotin ELISA) for viruses and bacteria infecting sweet potato and potatoes. Awards and Honors 1. 2. 3. 4. 2012-2013: USDA-ARS Outstanding performance award. 2011-2012: USDA-ARS Superior performance award. 2000: Chinese Ministry of Education Award for distinguished Ph.D. thesis in China. 1998: USDA-ARS Appreciation Award for "Molecular genetics study and the development of DNA marker linked to earworm resistance in corn". 5. 1993: World Bank Award for "Application of molecular methods and statistical analyses for discrimination of accessions and clones of vetiver grass". Oral presentations 1. Molecular characterization and detection of Mexican papita viroid. The American Phytopathological Society (APS) and the Mycological Society of America (MSA) – Joint Meeting. August 9-15, 2013, Austin, TX, USA 2. Mexican papita viroid: Molecular and biological characterization. 27th Tomato Disease Workshop. October 16-18, 2012. Wooster, Ohio, USA 3. Characterization and detection of Tomato necrotic stunt virus, a novel potyvirus infecting greenhouse tomatoes in Mexico. APS annual meeting. August 4-8, 2012, Providence, RI, USA 4. Overexpression of the Arabidopsis transcription factor REVOLUTA leads to increased soybean seed yield in replicated field trials. World Soybean Research VIII. August 10-15, 2009, Beijing, China 5. Overexpression of the Arabidopsis Transcription factor REVOLUTA leads to increased soybean seed size and yield. 2008 World Congress on In Vitro Biology. June 24-18, 2008, Tucson, Arizona, USA 6. Salicylic acid-induced transcription factor NtMYB1 regulates N gene-mediated virus resistance. 41st Annual Plant Development Workshop, November 20, 2007. Carleton University, Ottawa, Canada 7. Genomics of Sclerotinia sclerotiorum: expressed sequence tag analysis reveals genes associated with fungal pathogenesis. AAFC Fifth Annual Genomics Meeting. August 21-23, 2003, Saskatoon, SK, Canada. 8. Polygalacturonases of Sclerotinia sclerotiorum: expression during saprophytic and parasitic modes of growth. APS annual meeting, July27-31, 2002, Milwaukee, USA Students supervised Chen Rumei: Ph.D. student in plant pathology Department of Plant Pathology, China Agricultural University, Beijing, China. Thesis: Analysis of transgenic potato expressing dual PR proteins (PR1a and PR5) for their resistance to Phytophthora infestans infection. Shen Yuhuan: M.Sc. student in plant molecular biology The Graduate School of Chinese Academy of Agricultural Sciences, Beijing, China. Thesis: Cloning of sweet potato feathery mottle virus and sweet potato latent virus coat protein genes and development of RT-PCR detection technologies. Other individuals supervised 1. 2. 3. 4. Alan Wilder Andrea Gilliard Anna Butron Shuiqing Zhao 5. Ningfeng Wu 6. Guangchun Shi 7. Maolin Li 8. Yingying Sun 9. Li Zhu 10. Xiaoming Wu 11. Zhenglin Ju 12. Xiaomei Zhu 13. Aihong Xue small RNA library construction small RNA library construction DNA isolation and Southern hybridization PCR, RT-PCR, RNA isolation, mRNA purification, cDNA synthesis, and cDNA library construction Potato transformation Molecular cloning and PCR PCR-based DNA marker Potato transformation and virus detection PCR-based DNA marker PCR-based DNA marker Antibody purification and enzyme-labeling Antibody purification and enzyme-labeling Virus isolation and serological detection Publications in peer-reviewed journals 1. Li R, Ling K-S (2014) Development of RT-LAMP assay for rapid detection of an emerging potyvirus: tomato necrotic stunt virus. Journal of Virological Methods 200: 35-40 2. Ling K-S, Li R, Groth-Helms D, Assis-Filho F (2014) First report of potato spindle tuber viroid naturally infecting field tomatoes in the Dominican Republic. Plant Disease 98:701 3. Ling K-S, Li R, Zhang W (2014) First report of cucumber green mottle mosaic virus infecting greenhouse cucumber in Canada. Plant Disease 98:701 4. Li R, Fei Z, Ling K-S (2014) Molecular and biological properties of Tomato necrotic stunt virus and development of a sensitive real-time RT-PCR assay. Archives of Virology 159:353-358. 5. Li R, Gao S, Fei Z, Ling K-S (2013) Complete genome sequence of a new tobamovirus naturally infecting tomatoes in Mexico. Genome Announc. 1:e00794-13. 6. Ling K-S, Li R, Blesdsoe M (2013) Pepino mosaic virus genotype shifting in North America and development of specific RT-LAMP for rapid genotype identification. Virology Journal 10:117. 7. Ling K-S, Li R, Panthee DR, Gardner RG (2013) First report of potato spindle tuber viroid naturally infecting greenhouse tomatoes in North Carolina. Plant Disease 97:148 8. Li R, Gao S, Hernandez AG, Wechter WP, Fei Z, Ling K-S (2012) Deep Sequencing of Small RNAs in Tomato for Virus and Viroid Identification and Strain Differentiation. PLoS ONE 7:e37127 9. Li R, Bancroft B, Zhao SQ, Zheng SQ, Blahut-Beatty L, De Rocher J, Simmonds D (2011) Multiple inserts of gene of interest and selection marker gene are co-integrated and stably transmitted as a single genetic locus in transgenic soybean plants. In Vitro Cellular and Developmental Biology-Plant 47:274-281 10. Hegedus DD, Li R, Buchwaldt L, Parkin I, Whitwill S, Coutu C, Bekkaoui D, Rimmer S. R. (2008) Brassica napus possesses an expanded set of polygalacturonase inhibitor protein genes that are differentially regulated in response to Sclerotinia sclerotiorum infection, wounding and defense hormone treatment. Planta 228:241– 253 11. Li R, Rimmer R, Buchwaldt L, Sharpe AG, Seguin-Swartz G, Hegedus DD (2004) Interaction of Sclerotinia sclerotiorum with a resistant Brassica napus cultivar: expressed sequence tag analysis identifies genes associated with fungal pathogenesis. Fungal Genetics and Biology 41:735-753 12. Li R, Rimmer R, Buchwaldt L, Sharpe AG, Seguin-Swartz G, Hegedus DD (2004) Interaction of Sclerotinia sclerotiorum with Brassica napus: Cloning and characterization of endo- and exo-polygalacturonases expressed during saprophytic and parasitic modes. Fungal Genetics and Biology 41:754-765 13. Li R, Rimmer R, Yu M, Sharpe A, Hegedus DD (2003) Two Brassica napus PGIP genes are expressed at different levels in response to biotic and abiotic stresses. Planta 217:299-308 14. Butron A, Li R, Guo BZ, Widstrom NW, Snook ME, Cleveland TE, Lynch RE (2001) Molecular markers to increase corn earworm resistance in a maize population. Maydica 46:117-124 15. Guo BZ, Li R, Widstrom NW, Lynch RE, Cleveland TE (2001) Genetic variation within the maize population GT-MAS:gk and its relationship with resistance to Aspergillus flavus. Theor. Appl. Genet. 103:533-539 16. Guo BZ, Zhang ZJ, Li R, Widstrom NW, Snook ME, Lynch RE, Plaisted D (2001) RFLP markers associated with silk Maysin, antibiosis to corn earworm larvae (Lepidoptera: Noctuidae), in a dent and sweet corn cross. J. Economic Entomology 94:564-571 17. Yang Y, Li R, Qi M (2000) In vivo analysis of plant promoters and transcription factors by agroinfiltration of tobacco leaves. Plant Journal 22:543-551 18. Li R, Wu NF, Fan YL, Song BF (1999) Transgenic potato plants expressing osmotin gene inhibit fungal development in inoculated leaves. Chinese Journal of Biotechnology 15:71-75 19. Li R, Fan YL (1999) Reduction of lesion growth rate of late blight plant disease in transgenic potato plants expressing harpin protein. Science in China (Series C) 42:96101. 20. Wu XM, Wu NF, Qian XZ, Li R, Huang FH, Zhu L (1998) Phenotypic and genotypic changes in rapeseed after 18 years of storage and regeneration. Seed Science Research 8 (Suppl. 1):55-64. 21. Zhu L, Li R, Wu XM, Wu NF, Qian XZ, Fan YL (1998) RAPD analysis of genetic diversity among partial Chinese accessions of Brassica rapa. Chinese Biodiveristy 6(2):99-104 22. Wu NF, Li R, Wu XM, Zhu L, Fan YL, Qian XZ (1997) RAPD molecular markers and genetic diversity among 40 accessions of Brassica napus. Chinese Biodiversity 5:246-250 23. Ran JP, McFerson JR, Li R, Kresovich S, Lamboy WF (1995) Identities and relationships among Chinese vegetables Brassicas as determined by random amplified polymorphic DNA markers. J. Amer. Soc. Hort. Sci. 120:548-555 24. Kresovich S, Lamboy WF, Li R, Ren JP, Szewc-McFadden AK, Bliek SM (1994) Application of molecular methods and statistical analysis for discrimination of accessions and clones of vetiver grass. Crop Science 34:805-809 25. Li R, Cai SH, Liu J, Feng LX, Hsu H-T (1993) Monoclonal antibodies reacted specifically to potato virus X - HB strain (PVX-HB). Acta Phytopathologica Sinica 23:5-9 26. Li R, Guo J, Ju ZL, Zhu XM, Liang H, Wang YC (1992) Monoclonal antibodies against potato virus X and Y and their use in virus detection. Chinese Journal of Potato 6:233-236 27. Li R, Chiu WF (1992) Ultrastructural observation on Dahlia leaf tissue infected with dahlia mosaic virus. Acta Virologica Sinica 7:459-462 28. Li R, Xue A, Zhu XM, Cai SH (1992) Construction of hybridomas secreting monoclonal antibodies against sweet potato feathery mottle virus and use of antibody for detection of SPFMV. Chinese Journal of Biotechnology 8:277-281 29. Qiu BS, Zhao F, Wang XF Tian P, Li R, Cai S. (1992) Molecular cloning and application of cDNA probe of sweet potato feathery mottle virus. Acta Microbiologica Sinica 32:242-246 30. Li R, Zhu XM, Xue AH, Cai SH, Wang XF (1992) Identification of Langfang isolate of sweet potato feathery mottle virus. Acta Phytopathologica Sinica 22:319-322 31. Li R, Cai SH, Salazar LF (1990) Serological detection of viruses on sweet potato in China. Acta Phytopathologica Sinica 22:189-194 32. Li R, Chiu WF (1990) Three viruses associated with mosaic symptoms on Dahlia pinnata Cav in Beijing. Acta Phytopathologica Sinica 20:111-116 Literature Reviews 1. Li R, Fan YL (2000) Advances on genetic engineering of plant resistance against mycological diseases. Progress in Biotechnology 2:9-13. 2. Li R, Wu NF, Fan YL, Song BF (1997) Advances on resistance in potato against late blight disease. Chinese Journal of Potato 11:243-250 3. Li R (1995) Application of molecular diagnostics to plant genetic resources conservation. Chinese Biodiversity 3 (suppl.):22-29 4. Li R (1994) Molecular aspects of plant-pathogen interactions and strategies of disease control. Chinese Journal of Agricultural Biotechnology, 2:1-13 Proceedings, newsletters and bulletins 1. Li ML, Li R, Zhou MD, Eyzaguirre P, and Ayad WG (2000) Analysis of correlation between ethnobotany and genetic diversity of taro in China using RAPD assay. In: Proceedings of the Twelfth Symposium of the International Society for Tropical Root Crops (Eds: M. Nakatani and K. Komaki), P544-546. Tsukuba, Japan, 10-16 September 2000 2. Guo BZ, Butron A Li R, Zhang ZJ, Widstron NW, McMullen MD, Lynch RE (2000) Identification of a gene at the syntenic sh2-a1 region in maize. Maize Genetics Newsletter 74:64-66 3. Li R, Zhu L, Wu NF, Fan YL, Wu XM and Qian XZ (1997) Genetic variation and relatedness among oilseed accessions of Brassica juncea Czern. et Cross. Chinese Biotechnology Bulletin 5:26-31 4. Li R, McFerson JR and Kresovich S (1997) Genetic variation of acetohydroxyacide synthetase gene among cultivated Brassica species and its use in discriminating accessions of Brassica napus L. Plant Genetic Resources Newsletter 111:59-63 5. Li R, Wang YC, Cai SH, Zhang XD, Xie DX and Fan YL (1992) Monoclonal antibodies for detection bacteriocin M2 from strain M2 of Pseudomonas solanacearum. Chinese Science Bulletin 37:56-60 6. Li R, Xue AH, Zhu XM, and Cai SH (1992) Monoclonal antibodies to sweet potato feathery mottle virus. In: Agricultural Biotechnology. Proceedings of Asia-Pacific Conference on Agricultural Biotechnology, August 20-24, 1992, Beijing, China. China Science and Technology Publishing House, p.593-594 7. Li R, Cai SH, Liu J, Feng LX, and Hsu H-T (1990) The establishment of hybridomas secreting specific antibodies against HB strain of potato virus X by using mice with induced immunological tolerance to other strains of PVX. In: Potato and Sweet Potato Research in China, CAAS and CIP Region VIII. p. 51-56 Meeting Abstracts and Posters 1. Li R, Ling K-S. Screening tomato germplasm for resistance to Potato spindle tuber viroid. 2014 Tomato Breeder’s Round Table. Sepetmebr 14-17, 2014, Mills River, NC 2. Li R, Berendsen S, Fei Z, Ling K-S. Discovery of a new genotype of Squash mosaic virus through deep sequencing of small RNAs and development of a qRT-PCR for broad spectrum detection. APS-CPS Joint Meeting, August 9-13, 2014. Minneapolis, MN 3. Li R, Ling K-S (2013) Molecular characterization and detection of Mexican papita viroid. Phytopathology 103(Suppl. 2):S2.80 4. Ling K-S, Li R, Fei Z (2013) Global virus survey in tomato using small RNA deep sequencing technology. Acta phytopathologica sinica 43 (suppl.). 281-282 5. Ling K-S, Li R, Sombat S (2013) Characterization and detection of emerging viroids in North American greenhouse tomatoes. International workshop on viroids and satellite RNAs (IWVds). p. 5 6. Ling K-S, Li R, Bledsoe M (2013) Pepino mosaic virus genotype shift in North America and rapid genotype identification using loop-mediated isothermal amplification. Acta phytopathologica sinica 43 (suppl.). p. 402 7. Li R, Baysal-Gurel F, Kurowski C, Miller SA, Ling K (2013) Evaluation of disinfectants to control mechanical transmission of tomato viruses and viroids in greenhouse tomatoes. The 4th International Symposium on Tomato Diseases and 28th U.S. Annual Tomato Disease Workshop. p 30 8. Baysal-Gurel F, Li R, Ling K, Kurowski C, Miller SA (2013) Developing hygiene protocols against mechanically transmitted pathogens in greenhouse tomato production systems. The 4th International Symposium on Tomato Diseases and 28th U.S. Annual Tomato Disease Workshop. p 29 9. Ling K, Li R, Gao S, Fei Z (2013) Understanding the global distribution of tomato viruses and viroids using next-generation sequencing technology. The 4th International Symposium on Tomato Diseases and 28th U.S. Annual Tomato Disease Workshop. p 24 10. Li R, Ling K-S (2012) Molecular and biological characterization of Mexican papita viroid. 27th Annual Tomato Disease Workshop, P28 11. Baysal-Gurel F, Li R, Henderson DR, Ling K-S Miller SA (2012) Sensitivity of mechanically transmitted pathogens to different disinfectants. 27th Annual Tomato Disease Workshop, P29 12. Ling K-S, Li R, Panthee DR, Gardner RG (2012) Identification of Potato spindle tuber viroid naturally infecting greenhouse tomatoes in North Carolina and its potential in seed transmission. 27th Annual Tomato Disease Workshop, P27 13. Li R, Fei Z, Ling K (2012) Characterization and detection of Tomato necrotic stunt virus, a novel potyvirus infecting greenhouse tomatoes in Mexico. Phytopathology 102:S4.70 14. Ling K, Li R (2012) Genetic diversity and phylogenetic analysis revealed Pepino Mosaic Virus in North-America has shifted to a more aggressive CH2 genotype. Phytopathology 102:S4.71 15. Ling K, Li R, Wechter WP, Hernamdez AG, Fei Z (2012) Using small RNA technology to efficiently identify tomato viruses and viroids in mixed-infected field aamples. 26th Annual Tomato Disease Workshop. p 22 16. Li R, Bancroft B, Zhao SQ, Zheng SQ, Blahut-Beatty L, De Rocher J, Simmonds D (2010) Multiple inserts of gene of interest and selection marker gene are co-integrated and stably transmitted as a single genetic locus in transgenic soybean plants. 12th World Congress of the International Association for Plant Biotechnology, June 6-11, 2010, St. Louis, Missouri, USA 17. Li R, Bancroft B, Lum K, Nguyen T, De Rocher J, Simmonds D (2008) Overexpression of the Arabidopsis Transcription Factor REVOLUTA Leads to Increased Soybean Seed Size and Yield. In vitro Cellular and Developmental Biology, Volume 44, Issue Abstract, P-1012 18. Buchwaldt L, Li R, Hegedus DD, Rimmer SR (2005) Pathogenesis of Sclerotinia sclerotiorum in relation to screening for resistance. The XIIIth International Sclerotinia Workshop. 12-16 June 2005, Monterey, California, USA 19. Hegedus DD, Rimmer R, Buchwaldt L, Li R, Sharpe A (2005) Defining the biochemical mechanisms underlying resistance to stem rot disease (Sclerotinia sclerotiorum) in Brassica napus. The XIIIth International Sclerotinia Workshop. 1216 June 2005, Monterey, California, USA 20. Buchwaldt L, Hegedus D, Li R, Lydiate D, Rimmer R (2005) Preliminary mapping of physiological resistance to Sclerotinia sclerotiorum in Brassica napus. The XIIIth International Sclerotinia Workshop. 12-16 June 2005, Monterey, California, USA 21. Hegedus D, Rimmer R, Buchwaldt L, Li R, Sharpe A (2004) Gene expression associated with Sclerotinia infection and resistance in Brassica napus. 2nd Canadian Plant Genomics Workshop. August 29 - September 1, 2004, Québec City, Canada 22. Li R, Rimmer R, Sharpe A, Seguin-Swartz G, Hegedus D (2002) Polygalacturonases of Sclerotinia sclerotiorum: expression during saprophytic and parasitic modes of growth. Phytopathology 92:S47. Publication no. P-2002-0338-AMA 23. Li R, Rimmer R, Yu M, Gruber M, Sharpe A, Seguin-Swartz G, Hegedus D (2002) Brassica napus polygalacturonase inhibitor genes are differentially regulated by wounding and fungal infection. Phytopathology 92:S47. Publication no. P-20020339-AMA 24. Li R, Klessig DF, Yang Y (2000) Overexpression of a salicylic acid-inducible myb gene in transgenic tobacco enhances the N gene-mediated resistance to TMV. Phytopathology 90:S46. Publication no. P-2000-0330-AMA 25. Yang Y, Li R, Lee M (1999) Involvement of plant myb genes in disease resistance and susceptibility. 9th International Congress of Molecular Plant-Microbe Interactions, July 25-30, 1999, Amsterdam, the Netherlands 26. Guo BZ, Li R, Widstrom NW, Snook ME, McMullen MD, Lynch RE (1999) Preliminary identification and sequences of a gene in the sh2-a1 region that affects silk maysin concentration in maize silks. 41st Annual Maize Genetics Conference, March, 1999, Wisconsin, USA 27. Li R, Guo BZ, Widstrom NW, Lynch RE, Cleveland TE (1998) Genetic variation in maize germplasm GT-MAS:gk, as revealed by RAPD-PCR markers and the relationship to resistance to Aspergillus flavus. Phytopathology 88:S53. Publication no. P-1998-0384-AMA 28. Li R, Guo BZ, Widstrom NW, Lynch RE, Cleveland TE (1998) Genetic variation in GT-MAS:gk population and possible PCR and RFLP markers linked to resistance to Aspergillus flavus. Aflatoxin Elimination Workshop. Oct. 25-27, 1998, St. Louis, MO, USA 29. Guo BZ, Widstrom NW, Li R, Cong P, Lynch RE, Snook ME, Wilson DM, Cleveland TE (1998) Pyramiding resistance to Aspergillus flavus and corn earworm to control preharvest aflatoxin contamination: The possibility with genetic mapping and DNA markers linked to resistance traits. Aflatoxin Elimination Workshop. Oct. 25-27, 1998, St. Louis, MO, USA 30. Guo BZ, Li R, Cong P, Widstrom NW, Lynch RE, Wilson DM, Cleveland TE (1998) RFLP screening to map corn resistance to corn earworm and Aspergillus flavus. Aflatoxin Elimination Workshop. Oct. 25-27, 1998, St. Louis, MO, USA 31. Guo BZ, Li R, Cong P, Widstrom NW, Wiseman BR, Snook ME, Lynch RE (1998) Characterization of a chromosome region which enhance maysin production in silks and reduces corn growth and earn damage. Aflatoxin Elimination Workshop. Oct. 2527, 1998, St. Louis, MO, USA Book Translation 1. Li R and Zhang CY (1999) Methods in Plant Molecular biology and Biotechnology (Authors: Glick B. R. and Thompson J. E., published in 1993 by CRC Press, Inc., Boca Raton, Florida, U.S.A.), Chongqing Publishing House, Chongqing, China