Ahmad, Table S1, EJPP
Primers
name
sequence 5'-3' forward
PR1a
GAGGGCAGCCGTGCAA
PR1b
GGTCGGGCACGTTGCA
PR2b
CAACTTGCCATCACATTCTG
PR5
CCCCAACAAAACCTAGTGGA
PR4
CTAGCGGACCAGGGGAGAGC
PIN2
TGATGCCAAGGCTTGTACTAGAGA
PR7
(69B)
PR10
CAGCAGTCGGCCATGTAGCCAATGTT
AGGAGGTACGCTTCGATGGCCT
PAL
TTCAAGGCTACTCTGGC
ICS
TCGCCGGCATTCATTGGAAACA
CHS2
GGCCGGCGATTCTAGATCA
LoxD
CCATCTCTTATGGCCAGCAT
Pti4
GGATCAACAGTTACCACCG
TSR
ACCGGCGAAGGAGAAGTCGT
EF1
alpha
GGTTAAGATGATTCCCACC
sequence 5'-3' reverse
references
Herman et
CACATTTTTCCACCAACACATTG
al., 2007
Herman et
GATCCAGTTGCCTACAGGACATA
al., 2007
Van Kan et
CCAAAATGCTTCTCAAGCTC
al., 1992
F. Fiocchetti
ACCAGGGCAAGTAAATGTGC
et al., 2006
Gu et al.,
ACGCGATCAATGGCCGAAACAAG
2002
Herman et
AGCGGACTTCCTTCTGAACGT
al., 2008
Tian et al.,
GGGAGATGGTGGTGCTGGAAGC
2004
CACATTTTTCCACCAACACATTG
J. Peng et
CAAGCCATTGTGGAGAT
al., 2004
Uppalapati
AAAGCCCGTGCATCTTCTGT
et al., 2007
Schijlen et
TTTCGGGCTTTAGGCTCAGTT
al., 2007
Martinez de
GTGACAACACGTTTGGATCG
IIarduya et
al., 2008
Gu et al.,
CGGTGTTTCAGCCGCCGGA
2002
Zhang et al.,
GCGCCACTACAGGGGAGCAA
2004
Teyssier et
CCAGTTGGGTCCTTCTTG
al., 2008
Supplementary table S1 primers used for transcriptional expression study of 14 genes involved in defense
pathways in 3 mm flower buds and leaves of stolbur C and PO phytoplasma-infected tomatoes through real time
RT-PCR (except PR4 by semi-quantitative RT-PCR). PAL: Phenylalanine ammonialyase, ICS: Isochorismate
synthase, CHS2: Chalcone synthase 2, a-PRX: Acidic pathogenesis-related proteinX, b-PRX: Basic
pathogenesis-related proteinX, PRX: Pathogenesis-related proteinX, LOXD: Lipoxygenase, PIN2: Proteinase
inhibitor 2, PTI4: Transcription factor Pti4, TSR: stress-responsive factor TSRF1.
References for this table
Fiocchetti, F., Caruso, C., Bertini, L., Vitti, D., Saccardo, F. & Tucci, M. (2006). Over-expression of a
pathogenesis-related protein gene in transgenic tomato alters the transcription patterns of other defence
genes. The Journal of Horticultural Science & Biotechnology. 81 (1), 27-32.
Gu, Y-Q, Yang, C., Thara, V.K., Zhou, J., & Martin G.B. (2000). Pti4 Is Induced by Ethylene and Salicylic
Acid, and Its Product Is Phosphorylated by the Pto Kinase. The Plant Cell, 12(5), 771-785.
Ahmad, Table S1, EJPP
Herman, M. A., Davidson, J. K. & Smart C. D. (2008). Induction of plant defence gene expression by plant
activators and Pseudomonas syringae pv. tomato in greenhouse-grown tomatoes. Phytopathology, 98,
1226-1232.
Martinez de Ilarduya, O., Xie, Q. & Kaloshian, I. (2003). Aphid-induced defense responses in Mi-1-mediated
compatible and incompatible tomato interactions. Molecular Plant-Microbe Interactions, 16(8), 699‑
708.
Peng, J., Deng, X.., Huang, J., Jia, S., Miao, X. & Huang, Y. (2004). Role of Salicylic Acid in Tomato Defense
against Cotton Bollworm, Helicoverpa armigera Hubner. Z. Naturforsch. 59c, 856-862.
Teyssier, E., Bernacchia, G., Maury, S., How Kit, A., Stammitti-Bert, L., Rolin, D., et Gallusci, P. 2008. Tissue
dependent variations of DNA methylation and endoreduplication levels during tomato fruit
development and ripening. Planta, 228, 391-399.
Tian, M., Huitema, E., da Cunha, L., Torto-Alalibo, T. & Kamoun, S. (2004). A Kazal-like Extracellular Serine
Protease Inhibitor from Phytophthora infestans Targets the Tomato Pathogenesis-related Protease P69B.
Journal of Biological Chemistry, 279, 26370-26377.
Uppalapati, S. R., Ishiga, Y., Wangdi, T., Kunkel, B. N., Anand, A., Mysore, K. S., & Bender, C. L. (2007). The
Phytotoxin Coronatine Contributes to Pathogen Fitness and Is Required for Suppression of Salicylic
Acid Accumulation in Tomato Inoculated with Pseudomonas syringae pv. tomato DC3000. Molecular
Plant-Microbe Interactions, 20(8), 955‑965.
Van Kan, J. A. L., Joosten, M. H. A. J., Wagemakers, C. A. M., Berg-Velthuis, G. C. M. & Wit, P. J. G. M. 1992
Differential accumulation of mRNAs encoding extracellular and intracellular PR proteins in tomato
induced by virulent and avirulent races of Cladosporium fulvum. Plant Molecular Biology 20, 513-527.
Zhang, H., Zhang, D., Chen, J., Yang, Y., Huang, Z., Huang, D., Wang, X.C. & Huang, R. (2004) Tomato stressresponsive factor TSRF1 interacts with ethylene responsive element GCC box and regulates pathogen
resistance to Ralstonia solanacearum. Plant Molecular Biology, 55, 825-834.