Vontz Core Imaging Laboratory (VCIL)
University of Cincinnati
KODAK: CARESTREAM MOLECULAR IMAGING (CMI)
IN-VIVO MULTISPECTRAL IMAGING SYSTEM FX
We have two systems (they look identical) each with: 1) bioluminescence (BLI), 2) multispectral fluorescence, 3) x-ray, 4) planar radioisotopic imaging , and 5) surface optical.
**One system has an added 2D rotational software and hardware feature described below.
Small Animal Imaging System Specifications:
Fully integrated multi-modality bioluminescence and multi-spectral fluorescence imaging
system, optimized for in-vivo rodent imaging (mice and rats)
General Specifications:
 Cabinet system with single imaging chamber for all imaging modalities
 Light-tight chamber: fully functional in standard laboratory ambient light levels
 Illumination source(s): 2D: 175W Xenon and 3D: 400W Xenon
 Uniform light collection across FOV (<5% non-uniformity)
 Calibrated measurements (NIST-traceable)
 Camera: monochrome interlined CCD
 Self contained thermoelectrically super-cooled (-29°C) CCD (stable during changes in
ambient temperatures)
 Camera lens computer controlled 10X zoom, 20-200mm, f2.8-8.0
 Pixel Density: 2048 x 2048
 Resolution: 10 microns/pixel
 Pixel size 7.4 µm
 Acquisition software (Carestream Molecular Imaging Software-Carestream MI)
standard edition version 5.0.6.21
 Dynamic range: > 4.0 orders of magnitude
 Acquisition (16 bit single capture n-bit data acquisition)
 X and Y binning at 1x2, 2x2, 1x4, 2x4, 4x4 8x8, and 16x16 pixel options (increases
sensitivity)
 Minimum FOV range: 2cm x 2cm (high resolution) to 20cm x 20cm, continuous zoom
 Wide platform FOV can accommodate imaging of 2 rats (300g each) or up to 5 mice,
simultaneously
 Multiple exposure modes: Single capture, Multiple capture, Progressive exposure, Time
lapse Exposure
 Multi-spectral imaging using sets of filtered wavelengths to determine depth and
location
 Software-controlled filter selections
 OS: Windows2000/XP
 Automated hardware movement
 Hardware and imaging parameters software-controlled
 Preview display for positioning
Updated,2/8/2016
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Graphical user-interface for image acquisition
Pre-defined and saved full acquisition protocols for reproducibility and efficiency
Utilizes pre-defined linked acquisition and image analysis protocols for subsystems
System accepts and transfers experiment-specific information within image header
Designed for high throughput
Display interface for system status monitoring (temperatures, etc)
Continuous anesthesia and physiological monitoring system for 1-5 mice (see Animal
Care)
Bioluminescence (BLI) subsystem specifications:
 2D bioluminescence (or chemiluminescence) imaging
 Quantitative bioluminescence data
 Selectable/adjustable FOV and magnification
 Co-registration with X-ray or optical images (overlays)
 Depth discrimination
 High sensitivity (sub-pM detectibility; >85% efficiency for BLI; <70 photons/s/sr/cm2)
 Analysis software includes user-definable ROIs; quantification of photon counts;
distance and depth measures
 Calibration phantoms (simulate optical properties of tissue, with selectable light source)
Multiwavelength Fluorescence subsystem specifications:
 Multi-wavelength and multi-spectral 2D fluorescence imaging
 Quantitative fluorescence data
 Both shallow (subcutaneous) and depth (>1cm) fluorescence imaging
 Selectable/adjustable FOV and magnification
 Automated multiple-position excitation filter wheel with 28 excitation filters from 400760 nm in 10-20 nm increments (depth measurements)
 Automated multiple 4 position emission filter wheel with choice of 6 emission
interchangeable filters from 535-830nm
 Top and bottom illumination modes (selectable by a switch)
 Removal of background auto fluorescence (spectral unmixing)
 Co-registration with X-ray or optical images (overlays)
 Efficient detection of low light sources
 < 1 minute for typical 2D fluorescence acquisition
 Calibration phantoms (simulate optical properties of tissue, with selectable light source)
 Calibrated light source (with selectable wavelengths) for quality control testing
Digital X-ray subsystem specifications:
 Energy range: 12-35 kV
 Maximum Current: 150uA
 Spot size < 50 µ
 Cone of Illumination > 33degrees
 Automated 5 position x-ray filters for quantitative bone density analysis and fat/lean
imaging studies
 Beryllium filtration options of 0.1, 0.2, 0.4, and 0.8 mm
Updated,2/8/2016
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Target material: Tungsten
Non-uniformity correction with illumination reference
Radioisotope subsystem specifications:
 Patented radioisotopic phosphor screen to convert gamma rays and beta particles into
light photons for imaging
 Co-registration with X-ray or optical images (overlays)
 Detects variety of isotopes
Surface Optical Imaging/Reflectance/Absorbance subsystem specifications:
 White light transillumination
Image Processing Specifications:
 Full analysis and display packages (Carestream Multispectral-Carestream MS) version
1.3.0, Build 1081
 Software to fuse (overlay) bioluminescent, fluorescent, and radioisotope images with xray or optical
 Registration of selected bioluminescence and fluorescence data sets
 Composite images for display of multiple reporters
 Co-registration of imported CT and MRI images
 Large RAM (>1GB) on dedicated platform with speed and ability to handle large data
sets
 Analysis of longitudinal studies (including storage and use of pre-defined ROIs).
 Text or spreadsheet output of numerical data from analysis packages
 Image output with format options (TIFF, JPEG, etc.)
 Mouse atlas and tissue properties (optical characteristics)
 Large flat panel display
 High-end graphics card
Animal Care (during imaging):
 Anesthesia delivery system including isoflurane delivery & recovery
 Separate induction chamber
 Active anesthesia scavenge from animal chamber
 Multiple animal manifolds within imaging chamber (up to 5 mice and 2 rats)
 Multiple Animal physiological monitoring system with ECG, respiration, temperature
(SAII-MA-ERT)
 All potential animal-contact components can be easily disinfected
 Circulating warm air through imaging chamber for animal temperature maintenance
**Additional Hardware and Software included with one of the 2D systems:
Multiple Angle 2D Rotational Imaging (sometimes called “3D”) subsystem specifications:
 Rotary imaging stage for rotational (2D) cine imaging
 Rotational imaging software, all hardware computer controlled
 May be used in any combination of all 5 modalities
Updated,2/8/2016
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Single acquisition, mice only
<2mm spatial resolution
High speed scanning
High-speed final image recon
Updated,2/8/2016