CURRICULUM VITAE Jyothirmai Madhavi. K kjm.agri@gmail.com ph: +91-9848049064 Office Residence Scientist, Plant Pathology Nizampet Road, Kukatpally AICRP –Sub Tropical Fruits Hyderabad-500072 Andhra Pradesh, INDIA Fruit Research Station SangareddyAndhra Pradesh, INDIA ____________________________________________________________________________ EDUCATION: Ph.D. (Agriculture) with Plant Pathology / Virology as a specialization from Agricultural College, Bapatla, Acharya N. G. Ranga Agricultural University, India M.Sc. (Agriculture) with Plant Pathology as a specialization from College of Agriculture, Rajendranagar, Acharya N. G. Ranga Agricultural University, Hyderabad, India B.Sc. (Agriculture) from Agricultural College, Bapatla, Acharya N. G. Ranga Agricultural University, India OTHER ACHIEVEMENTS: Rajiv Gandhi National Fellowship (RGNF) from Union Grant Commission (UGC) for PhD program. Qualified Counsel of Scientific and Industrial Research(CSIR) – National Eligibility Test(NET) Qualified Indian Counsel of Agricultural Research(ICAR)- National Eligibility Test(NET) Undergone training on “Serological and Nucleic acid Based Methods for the detection of Plant Viruses”, at ICRISAT conducted by Department of International Development (DFID), UK and ICRISAT, Patancheru, India. HISTORY 2009 to till date 2006-2009 : Assistant Professor cum Scientist at Dr YSR Horticulture University : Junior Research Fellow, Union Grants Commission- Rajiv Gandhi National fellowship at National Bureau of Plant Genetic Resources (NBPGR), Regional Station, Hyderabad on “Identification and characterization of Peanut bud necrosis virus (PBNV) and Tobacco streak virus (TSV) on 1 blackgram (Vigna mungo L. Hepper) and greengram (Vigna radiata L. Wilczek)”. 2003-2004 : Senior Research Fellow, Indian Counsel of Agricultural Research (ICAR) at National Bureau of Plant Genetic Resources(NBPGR), Regional Station, Hyderabad in NATP ROPS-18 Project entitled, “An Integrated Approach to control Stem Necrosis Disease of Groundnut” from 26-08-03 to 30-09-04, which was in collaboration with International Crop Research Institute for Semi Arid Tropics (ICRISAT), National Research Centre on Groundnut (NRCG), Junagarh and Acharya N G Ranga Agricultural University (ANGRAU). PRESENT OCCUPATION: - Assistant Professor cum Scientist (Plant Pathology) at Dr. YSR Horticultural University (separated from Acharya NG Ranga Agricultural University in 2007) from 26-11-2009 to till date. TEACHING: Assistant Professor, Plant Pathology at Horticultural College and Research Institute, Anantarajupeta from 26-11-2009 to 31-7-2012. The courses offered for B.Sc. (Hons.)Horticulture S. No. Course no. and Course Title Credit hours 1 PATH 1.7.1 (2+1) Fundamentals of Plant Pathology 2 PATH 2.7.1 (2+1) Diseases of Fruits, Plantation, Medicinal and Aromatic Crops 3 PATH 3.7.1 (2+1) Diseases of Vegetable, Ornamental and Spice Crops 4 PATH 1.7.2 (0+1) Mushroom Culture 5 PPBM 1.10.2 (1+1) Introductory Microbiology 6 ENTO 2.6.1(0+1) Nematode pests of Horticultural Crops and their management 7 Hands on Training/ Nursery Production and Management: Experimental Diseases and their Management Learning Program ELP-401 (1+1) 8 Hands on Training/ Protected Cultivation of High Value Experimental Horticultural Crops: Diseases and their Learning Program Management Year I II III I I II IV IV 2 ELP-402 (1+1) Other activities in Teaching: Prepared Lab manuals for the following courses o Introductory Microbiology o Nematode pests of Horticultural Crops and their management o Protected Cultivation of High Value Horticultural Crops: Diseases and their Management o Nursery Production and Management: Diseases and their Management External question paper moderation for the following courses at University level o Fundamentals of Plant Pathology o Diseases of Fruits, Plantation, Medicinal and Aromatic Crops o Diseases of Vegetable, Ornamental and Spice Crops o Introductory Microbiology Guiding two Post Graduate Students as member of the Advisory Committee Advisor for 16 Under Graduate students. Associate Dean Representative for Rural Agricultural Work Experience Programme (RAWEP) for Fourth Year students Acted as In-charge of Institute Library RESEARCH: 1. Scientist, Plant Pathology in All India Coordinated Project on Sub Tropical Fruits (AICRPSTF) at Fruit Research Station, Sangareddy from 06-08-2012 to till date. Ongoing AICRP-STF Projects-Plant Pathology A. Screening of mango germplasm against powdery mildew Objective: To identify field resistant mango varieties / hybrids against powdery mildew o Powdery mildew is one of the worst diseases of mango devastating most of the commercial mango varieties at flowering and fruit setting stages in winter season. Therefore identifying source of resistant will be useful for developing powdery mildew field tolerant lines for producing quality mangoes free from fungicidal residues for exports. o Periodical inspection of mango varieties and hybrids at flowering and fruit setting stages of the crop will be done. o Intensity of powdery mildew attack will be recorded as per the scale. o The cultivar which show resistant reaction in the previous year will be tested by artificially inoculating with powdery mildew spores during the subsequent year to confirm their resistance. 3 B. Epidemiological studies of mango powdery mildew Obectives: To study the relationship with temperature, relative humidity and rainfall or appearance of the disease in susceptible mango cultivars. o Farmers can be benefited by effective fore warning system for taking up timely sprays of fungicides for protecting the mango crop against powdery mildew attack. C. Studies on malformation of mango Obectives: i. To establish the pathological nature of the disease, using in situ grafting and seed transmission techniques. ii. To know the intensity of floral malformation in mango cultivars. iii. Monitoring of the disease during November to February (Flowering season) and off-season flowering. D. Management of mango anthracnose Obectives: To evaluate the different fungicides by spraying for protecting the crop against mango anthracnose, leaf spot, blossom blight and fruit spot. E. Survey of seasonal occurrence of different diseases of mango Obectives: i. To record the incidence of different diseases of mango in various Agro climatic regions of Andhra Pradesh. ii. Survey (Roving and fixed plot ) will be conducted in major mango growing areas of AP F. Management of Blossom blight of mango Obectives: To evaluate the different fungicides by spraying for protecting the crop against mango blossom blight. G. Cost effective management of post harvest anthracnose of mango by pre and post harvest treatments Obectives: To evaluate the effective cost management by Preharvest spray (fungicides) and post harvest treatment (hot water treatments) against mango anthracnose disease. 2. Scientist in-charge for Entomology as post of Scientist, Entomology is vacant Ongoing AICRP-STF Projects- Entomology A. Screening of germplasm against pests of mango (stone weevil, hoppers, thrips, scales, mealybugs) 4 Obectives: i. To screen the existing germplasm at FRS, Sangareddy for stone weevil. ii. To screen the new entries of mango at FRS, Sangareddy for hoppers, thrips, scales and Mealy bugs B. Population dynamics of major pests of mango (hoppers and fruit flies) Objective: Fortnightly observations will be recorded on 10 panicles/twigs of five trees for hopper and monitoring of fruit fly at weekly intervals using Methyl eugenol traps. Systematic surveillance is to be done for hoppers and fruit flies and correlation with biotic and abiotic factors is to be established. C. Integrated management of mango hopper Objective: To schedule the IPM model for hopper management. The experiment was proposed to be laid out with the following treatments. Module I : First spray of Beauveria bassiana @ 1 x 10 7 spores /ml) at panicle emergence stage followed by second spray (after 15 days of first spray ) of Verticillium lecanii ( @ 1 X 107 spores/ml). Third need based spray of Neem Azal (10000 ppm @ 3 ml/lit. of water). Module II : First spray of Neem Azal (10000 ppm @ 3 ml/lit. of water) at panicle emergence stage followed by second spray (after 15 days of first spray) with Nimbicidene (1500 ppm @ 3ml /lit of water) Third spray of Neem Azal (10000 ppm @ 3 ml/lit. of water after 15 days of second spray) and fourth need based spray with Nimbicidene (1500 ppm @ 3ml/lit of water). Module III : First spray of thiamethoxam (0.008 %) at panicle emergence stage followed by second spray (21 days after first spray ) of profenophos (0.05%) and third need based spray of carbaryl (0.15%) Module IV : First spray of spinosad (0.004%) at panicle emergence stage followed by second spray (21 days after first spray) with thiamethoxam (0.008%) and third need based spray of Neem Azal (10000 ppm @ 3 ml /lit of water. Module V: First spray of Acephate (0.04%) at panicle emergence stage followed by second spray (21 days after first spray) with spinosad (0.004%) and third need based spray of carbaryl (0.15%). Control : Untreated D. Survey and surveillance of pollinators Objective: Tagging of 50 panicles per tree and observation of pollinators at different hights and directions of the plant. E. Cost effective management strategies of fruit flies in mango Objective: To evolve cost effective management strategy for fruit flies by using 5 i. Hanging of wooden block (5 X 5X 1 cm) in plastic bottle soaked in solution in ratio of 6:4:1 (alcohol : methyl eugenol : DDVP) used @ 10 traps / ha (Replace the wooden block at 2 month interval). ii. Hanging of glass wide mouth bottle trap containing 0.1% methyl eugenol and 0.1% DDVP @ 10 traps /ha. (Solution should be changed at weekly interval) iii. Hanging of Rakshak (Dapoli) trap with methyl eugenol lure @ 5 traps / ha (Replace the lure at 2 months interval). Scientist incharge for Rural Awareness Working Experience Programme (RAWEP 2012-13) RAWEP for under graduate students of Horticulture is offered during 7 th semester, which is a 90days programme with an objective to provide opportunity for practical training at farmer level in crop production and plant protection through work experience. Primary contact person for the students, selection of villages and host farmers for the students in the vicinity of the Research Station, clarifying the doubts of students, maintaining the performance sheets of students, helping in conducting the extension activities at their respective villages, arranging minikits, method demonstration plots in villages, conducting Farmer Exhibition at village, weekly visits to villages etc. Conducted hands on training programme on Mass multiplication of fungal and bacterial biocontrol agents for the students. Internal evaluation of the students, VIVA-VOSE on last working day, internal and external evaluation of Research Station activities. EXTENSION: Farmer Training and Awareness programmes on Pest and Disease Management in Mango and Guava. Delivered lectures at Horticultural Training Institute, Department of Horticulture, Government of Andhra Pradesh on Pest and Disease Management in fruit crops, mango, guava, papaya, grape etc. Resource person at Farmers Training Center, National Horticultural Mission, Ootukur, Kadapa on Integrated Pest Management in fruit crops. Radio talks on All India Radio on Pest and Disease Management in Mango, Guava, Papaya and vegetables. Field surveys to farmer fields and interaction with the farmers and recommendations of IPM. Subject Matter Specialist at the Farmer Motivation Tours (Rythu Chaitanya Yatralu) by Department of Agriculture, Government of Andhra Pradesh, to villages every year. EXPERIENCE AS SENIOR RESEARCH FELLOW (SRF): 6 Worked as Senior Research Fellow (SRF) in NATP ROPS-18 Project entitled, “An Integrated Approach to control Stem Necrosis Disease of Groundnut” from 26-08-03 to 30-09-04, which was in collaboration with International Crop Research Institute for Semi Arid Tropics (ICRISAT), National Research Centre on Groundnut (NRCG), Junagarh and Acharya N G Ranga Agricultural University (ANGRAU). Monitoring surveys were conducted for the incidence of peanut stem necrosis disease (PSND) caused by Tobacco streak virus (TSV) and Peanut Bud Necrosis Disease (PBND) in Anantapur District. TSV and thrips association and role of infective pollen in PSND spread was established. Precise differentiation of PSND and PBND under field conditions was made, although both produce necrotic symptoms. About 10,000 samples were tested by ELISA for PSND and PBND. Seed transmission studies were conducted in sunflower, groundnut, soybean, parthenium, marigold, cowpea, chenopodium and gomphrena. Host range studies were conducted in 25 crop and weed species. Molecular, biochemical and biological characterization of plant pathogens viz., fungi , viruses (DNA / RNA isolation, PCR / RT-PCR, Cloning, sequencing) in vivo and in vitro screening histo chemical studies (microtomy), biochemical studies (total phenols and total sugars), molecular studies (isozymes, PR-proteins and PCRRAPDs). TECHNICAL SKILLS: Basic laboratory techniques of Plat Pathology: Pathogen isolation, purification, pathogenicity testing, culturing, pure culture maintenance, preserving the pathogen cultures and revival, gram staining of bacteria, fungicidal studies and microscopic studies. Histochemical techniques: Fixation, dehydration, infiltration and paraffin embedding, microtomy, staining of paraffin sections and mounting. Biochemical techniques: Extraction and quantification of total sugars, phenols and proteins. Molecular techniques: Polyacrylamide gel electrophoresis (PAGE), SDS and Native PAGEs, Isozyme analysis of enzymes and pathogen related proteins (PR-proteins), Genomic DNA isolation and purification of plant as well as fungus, gel electrophoresis, PCR, PCR-RAPDs, RTPCR, gene cloning, sequencing Virology techniques: Mechanical transmission, Graft transmission Vector transmission Host range studies by artificial and natural infection conditions 7 maintenance of cultures on wide host range under glass house conditions, screening of germplasm for resistance under glasshouse and field conditions virus-vector relationships thrip transmission studies of Tobacco streak virus (TSV), and Peanut bud necrosis virus (PBNV), Aphid transmission studies of Cowpea mosaic virus (BICMV), whitefly transmission studies of tomato leaf curl virus, Pollen transmission studies of tobacco streak virus from parthenium, sunflower, tridax etc. Serological techniques- Enzyme Linked Immuno Sorbent Assay (ELISA) (DAC & DAS), Dot blot immuno assays, Detection of plant viruses in import and export germplasm, Molecular characterization of Plant viruses – RNA isolation, RT-PCR, Coat protein cloning and sequencing analysis, western blotting. COMPUTER SKILLS: Bio-informatics skills, BioEdit multiple sequence analysis, tree view, clustalX, clustalW, MS Office and internet applications. DOCTORAL AND POST GRADUATE RESEARCH : DOCTORAL (PhD) THESIS TITLE: Identification and characterization of Peanut bud necrosis virus (PBNV) and Tobacco streak virus (TSV) on blackgram (Vigna mungo L. Hepper) and greengram (Vigna radiata L. Wilczek). Thesis Abstract: Blackgram and greengram have been subjected to the attack of several pathogens such as fungi, bacteria and viruses. Among the major diseases of blackgram and greengram, necrosis is most important.The two important viruses reported to cause necrosis in blackgram and greengram are PBNV and TSV. Although both the viruses cause necrotic symptoms and are transmitted by thrips, the method of transmission and the virus vector relationship vary and hence need different approaches of management practices. It is necessary to identify and differentiate necrosis-causing virus on blackgram and greengram to follow the appropriate management practice. Keeping this in view following research work has been taken up. Surveys have been carried out in major blackgram and greengram growing areas. Upon mechanical inoculations, PBNV and TSV isolates of blackgram and greengram infected 18 and 25, respectively, out of 36 plant species tested. Under natural conditions, out of 44 suspected as well as random plant species collected and tested by DAC-ELISA, PBNV was detected in 13 samples and TSV was in 19, comprising economically important crops and several weeds. TSV-BG and TSV-GG infections on blackgram could be 8 differentiated from PBNV-BG and PBNV-GG infections by exhibiting smalling of leaves with mosaic mottling and stunting in addition to the symptoms produced by PBNV. Quasi spherical virus particle measuring 80-110 nm and 25-30 nm diameters, were observed in the leaf dip preparations of PBNV and TSV isolates of blackgram and greengram by electron microscopy, respectively. PBNV and TSV isolates of blackgram and greengram were found to be not transmitted by seed in blackgram and greengram. Transmission of PBNV isolates of blackgram and greengram by Thrip palmi was determined. Pollen associated thrip transmission of TSV-BG and TSV-GG by the four species viz. Frakliniella schultzei, Scirtothrips dorslis, Thrips palmi and Megalurothrips usitatus was ascertained by depositing the pollen from infected parthenium on leaves of blackgram and greengram and colonized by any of vector species. The complete nucleotide sequences of nucleo capsid (N) gene of PBNV-BG and PBNV-GG isolates and coat protein (Cp) gene of TSV-BG and TSV-GG isolates were determined. The sequenced region in PBNV isolates of blackgram and greengram contained a single open reading frame of 831 bases that could potentially code for a protein of 276 amino acids while the sequenced fragment of TSV isolates of both the crops contained a single open reading frame of 717 bases that could encode for a protein of 238 amino acids. Comparative sequence analysis revealed that PBNV-BG isolate shared hundred per cent sequence identity with PBNV-GG at amino acid levels and 99.7% at nucleotide level, whereas TSV-BG isolate shared hundred per cent sequence identity with TSV-GG at nucleotide as well as amino acid levels. The nucleo capsid (N) gene and coat protein (Cp) gene sequences of PBNV and TSV isolates of blackgram and greengram shared the sequence homology above 90% with the corresponding gene sequence of other PBNV and TSV isolates from India from different hosts, respectively. Hence, it is proposed that the PBNV and TSV isolates of blackgram and greengram should be regarded as strains of PBNV and TSV already existing in India, respectively. Fifty entries of blackgram and greengram screened against necrosis disease, under field conditions in Anantapur and Tandur all entries showed susceptibility with varied per cent mortality. Forty Blackgram and 34 greengram genotypes screened under glasshouse conditions by mechanical inoculation with PBNV and TSV showed hundred per cent disease infection while the per cent mortality was more in TSV infections in both the crops. POST GRADUTE (MSC.(Agriculture)) THESIS TITLE: Biochemical aspects of resistance against Alternria helianthi(Hansf.) Tubaki and Nishihara in cultivated and wild sunflower (Helianthus spp.). Thesis Abstract: Sunflower stands next to the groundnut, mustard and rapeseed in terms of importance among the oilseed crops by having low cholesterol content. Successful cultivation of 9 sunflower in India is limited by the susceptibility to biotic and abiotic factors. Among them, diseases caused by pathogenic fungi cause considerable yield loss. Of these, leaf spot/blight incited by A. helianthi is one of the important foliar diseases. No cultivated variety is resistant to A. helianthi. Use of systemic and non-systemic fungicides is not economical and causes environmental pollution and residue problems. Development of resistant varieties is the appropriate approach to control the disease where wild sunflowers are the potential donors for resistance. Keeping these points in view, six wild Helianthus species of different ploidy levels were studied for resistance against A. helianthi in comparison with highly susceptible cultivated sunflower cv. CO-4 by different means viz., in vivo and in vitro screening histo chemical studies (microtomy), biochemical studies (total phenols and total sugars), molecular studies (isozymes, PR-proteins and PCR-RAPDs). Among the wild sunflowers studied, H. occidentalis (diploid), H. hirsutus (tetraploid) and H. tuberosus (hexaploid) showed resistant reaction to A. helianthi and thus suggested to breeders for further breeding programs for interspesific hybridization to obtain resistance against A.helianthi. EXTRACURRICULAR ACTIVITIES: Student editor of college magazine. Participated in intercollegiate cultural meets and represented the college in national youth festivals. PUBLICATIONS: 1. Jyothirmai Madhavi K, Prasada Rao RDVJ and Subbarao M 2011 Pollen associated thrip transmission of Tobacco streak virus in blackgram and greengram. Indian Journal of Plant Protection 39(1&2):54-59. 2. Prasada Rao RDVJ, Jyothirmai Madhavi K, AS Reddy, Varaprasad K S, Nigam S S, Sharma K K, P Lava Kumar and Waliyar F 2009 Non-transmission of Tobacco streak virus isolate occurring in India through the seeds of some crop and weed hosts. Indian Journal of Plant Protection 37(1&2):92-96 3. P Lava Kumar, Prasada Rao RDVJ, Reddy AS, Jyothirmai Madhavi K, Anitha K and Waliyar F 2008 Emergence and spread of Tobacco streak virus Menace in India and Control Strategies. Indian Journal of Plant Protection 36:1-8 4. Sanatalakshmi Prasad M, Jyothirmai Madhavi K, Prasada Rao R D V J, Harivir Singh and Marimuthu 2005 Incidence of Tobacco streak virus on sun hemp (Crotalaria juncea L.). Indian Journal of Plant Protection 33:297 5. Prasada Rao R D V J, Jyothirmai Madhavi K, Vara Prasad K S and Khetarpal R 2005 Present status of stem necrosis disease of groundnut caused by Tobacco Streak Virus. Indian Journal of Virology 16:66 6. Madhavi K J, Sujatha M, Rajaram Reddy D and Chander Rao S 2005 Biochemical characterization of resistance against Alternaria helianthi in cultivated and wild sunflower Helia 28:13-24 10 7. Madhavi K J, Sujatha M, Rajaram Reddy D and Chander Rao S 2005 Cultural characteristics and histological changes in leaf tissues of cultivated and wild sunflower infected with Alternaria helianthi. Helia 28:1-12 SYMPOSIA/CONFERENCES: 1. Jyothirmai Madhavi K, Agarwal S, Prasada Rao R D V J, Subbarao M, Lal Ahmed M and Jain R K 2013 Diagnosis and Molecular Characterization of Viruses Expressing Similar Necrotic Symptoms in Blackgram (Vigna mungo L. Hepper) and Greengram (Vigna radiata L. Wilczek) for “12th International Plant Virus Epidemiology Symposium (IPVE)” held at Arusha, Tanzania during January 28 to February 1, 2013. 2. Jyothirmai Madhavi K, Prasada Rao RDVJ, Subbarao M and Lal Ahamed M 2012 Diagnosis, Biological and Serological Characterization of Viruses Expressing Similar Necrotic Symptoms in Blackgram and Greengram” at International Conference on Plant Health Management for Food Security at DRR, Rajendranagar, Hyderabad, India during November 28-30, 2012. 3. Jyotsna M K, Jyothirmai Madhavi K, Anitha K , Siva Prasad Y, Bhaskar Reddy B V and Prasada Rao RDVJ 2012 Serological and molecular detection of Tobacco streak virus(TSV) transmitting non- systemically in Cotton in Andhra Pradesh, India at International Conference on Plant Health Management for Food Security at DRR, Rajendranagar, Hyderabad, India during November 28-30, 2012. 4. Jyothirmai Madhavi K, Prasada Rao R D V J and Subbarao M and Lal Ahamed M 2012 Patterns of Thrip Transmission of Viruses Causing Necrosis Disease in Blackgram (Vigna mungo L. Hepper) and Greengram (Vigna radiata L. Wilczek) at 3rd GLOBAL CONFERENCE: Plant Pathology for Food Security by Indian Society of Mycology and Plant Pathology (ISMPP) at Udaipur, India, January 10-13, 2012. 5. Jyothirmai Madhavi K, Agarwal S, Prasada Rao R D V J, Subbarao M and Jain R K 2012 Sequence homology of CP genes of Tobacco streak virus (TSV) isolates causing necrosis disease in blackgram and greengram in Andhra Pradesh and phylogenic relationships with the TSV isolates reported from India” at 3 rd GLOBAL CONFERENCE: Plant Pathology for Food Security by Indian Society of Mycology and Plant Pathology (ISMPP) at Udaipur, India, January 10-13, 2012. 6. Jyothirmai Madhavi K, Agarwal S, Prasada Rao R D V J, Subbarao M and Jain R K 2011 Sequence homology of N genes of Peanut bud necrosis virus (PBNV) isolates causing necrosis disease in blackgram and greengram in Andhra Pradesh and phylogenic relationships with the PBNV isolates reported from India at VIROCON2011: Virology Conference 2011 by Indian Virological Society (IVS) at National Research Centre on Equines, Hisar, India. 11 REFERENCES: 1. Dr. P. Lava Kumar Head, Germplasm Health Unit International Institute of Tropical Agriculture (IITA) PMB 5320, Ibadan, Nigeria Mobile: +234 7032565130 www.iita.org; L.kumar@cgiar.org 2. Dr. M. Sujatha Principal Scientist, Crop Improvement, Directorate Oilseed Research (DOR), Rajendranagar, Hyderabad-30. Phone: Off: 040-24015345 Ext 101 Mobile: +91 9440880335; mulpurisujata@yahoo.com 3. Dr. K. Anitha, Principal Scientist, Plant Pathology, NBPGR-RS, Rajendranagar, Hyderabad-30. Phone: Off: 040-24014072 Mobile: +91 9440116873 aniyagnya@yahoo.com 12