Chapter 4 – Review All cells need energy in a form of ATP ATP is produced when cells break down molecules of glucose Plants can produce their own glucose by process of photosynthesis Animals and humans need to get glucose in their food. ATP = adenosine triphosphate (3 phosphate groups = energy rich molecule), fuel for all cell processes. ADP = adenosine diphosphate (2 phosphate groups = energy poor molecule) ATP ADP + P (one phosphate group) + energy (used by cell) ADP + P + energy ATP Photosynthesis: Plants convert the sunlight energy, carbon dioxide and water into glucose (energy rich carbohydrate) 6 CO2 + 6 H2O + sunlight energy C6H12O6 + 6 O2 Light energy – energy from the sunlight, used by plants for photosynthesis CHLOROPLAST Outer membrane Thylakoid: coin-shaped, membrane enclosed compartment. Membrane contains pigment chlorophyll Granum (pl. grana): stack of thylakoids Inner membrane Chlorophyll: Pigment found on the thylakoid membrane. Two types – chlorophyll a and chlorophyll b. They absorb most of the red and blue wavelengths of sunlight. Green is being reflected = plants appear to be green. Stroma (fluid) Step 1 of photosynthesis: Light-dependent reaction (happens during daylight!) Happens in thylakoids of chloroplasts Chlorophyll absorbs sunlight Water molecule is broken down Oxygen is released Energy carried along thylakoid membrane is transferred to ATP (energy-rich molecule) Final products: ATP molecules (enter step 2 of photosynthesis) and oxygen (released outside the plants) Chemical energy – energy in the bonds of molecules like sugar. This energy is released when bonds are broken and is used to produce ATP. Step 2 of photosynthesis: Light- independent reaction (happens day or night) Happens in stroma of chloroplasts Needs ATP (from step 1) and CO2 (carbon dioxide) CO2 molecules are added together in a cycle of chemical reactions, producing larger carbon molecule Energy (ATP) from step 1 is used Molecule of glucose (simple sugar) is produced Final product: glucose (stores chemical energy) Chemosynthesis: uses chemical energy (instead of sunlight energy to produce energy-rich molecules like sugar. Remember organisms deep down on the ocean floor, near hydrothermal vents? This review is intended to help you study for upcoming test. To be fully prepared and ready, you should still review the book, your notes, section and chapter review worksheets and any information you accumulated during this chapter (including yeast lab). How do cell break down glucose to produce needed ATP? CELLULAR RESPIRATION Glucose Releases chemical energy from sugars (and other carbon based molecules) to make ATP when oxygen is present (aerobic process). Happens in mitochondria. Think opposite to photosynthesis C6H12O6 + 6 O2 6 CO2 + 6 H2O + energy (ATP) Oxygen Outer membrane Mitochondria Glycolysis: Matrix – fluid inside of mitochondria Splits glucose molecule into two 3-carbon molecules (pyruvates), 2 ATP and extra energy is packaged into energy-carrying molecules (NAD+) NAD+ + energy = NADH Anaerobic process (no O2 needed) Happens in cytoplasm Final products: o pyruvates (enters next stage), o 2 ATP (energy ready for cell to use= $1 bill), o NADH (energy-carrying molecule, enters next stage, $20 bill) Stage 1 of cellular respiration (Krebs cycle) 3-carbon molecules are broken down in a cycle of chemical reactions Final products: o Small number of ATP o CO2 (released out) o Energy-carrying molecules (like NADH), they enter 2nd stage of CR Inner membrane – stage 2 of cellular respiration (electron transport) Uses energy-carrying molecules from stage 1 and oxygen to produce more ATP molecules Energy from energy-carrying molecules is transferred to chains of proteins in the membrane Final products: 34-36 ATP molecules, water and heat Glycolysis (2 ATP) + Cellular Respiration (34-36 ATP) = total of 36-38 ATP No Oxygen FERMENTATION Anaerobic process DOES NOT PRODUCE ATP! Recycles NAD+ (energycarrying molecules) Without fermentation all NAD+ would be used up and glycolysis would stop. Many different cells (like yeast) use fermentation. Thanks to them we have bread, cheese, yogurt, alcohol. Different end products of fermentation give different taste and texture to food. Lactic acid fermentation: glucose pyruvate lactic acid Waste product (lactic acid) is what causes our muscles to burn after strenuous exercise. It also contributes to sour taste of milk and yogurt Alcoholic fermentation: glucose pyruvate alcohol + CO2 Yeast uses alcoholic fermentation during bread production. CO2 causes dough rise. During baking CO2 evaporates and yeast is killed by heat. Glycolysis (2ATP) + Fermentation (no ATP) = total of 2 ATP