Supplements
Dynamics of the Heat Stress Response of Ceramides with Different Fatty-Acyl
Chain Lengths in Baker’s Yeast
Po-Wei Chen, Luis L. Fonseca, Yusuf A. Hannun, Eberhard O. Voit
S5 Text: Impact of data variability on estimated distributions of dynamic fluxes
Results shown in the main text are calculated based on interpolation of mean of the duplicate raw data
(Figure 10). In order to assess the impact of data variability, we performed the following step-wise analysis:
A. Adopt the original, smoothed and interpolated mean data as the baseline concentration.
B. Sample a uniformly distributed random variable from the range [1/1.5, 1.5].
C. Perturb the original dataset from time point 0 to 30 by the drawn random number and repeat
for 1000 simulations.
D. For each set of randomly perturbed data, redo the simulation in the “Methods” Section to
obtain dynamic fluxes.
E. Compare the fluxes based on perturbed data with the originally estimated fluxes.
The perturbed data are presented in Figure S4.1, and the perturbed flux distributions are shown in Figure
S4.2. Gray dots, blue lines, blue bars and black asterisks represent single simulations, mean values, 20th
and 80th percentiles, and median values, respectively. The flux distributions obtained from perturbed data
are similar to the originally estimated fluxes (used in the main text). Increased variances are observed, but
they do not influence the further estimation of enzyme activities.
C16 DHC
0.02
12
x 10
-3
C18 DHC
C18:1 DHC
0.014
0.01
8
0.008
6
0.006
0.01
0.004
0.005
4
0
0
2
x 10
10
-3
20
30
8
1.5
6
1
4
0.5
2
-4
C24 DHC
4
6
3
4
2
2
1
x 10
10
-4
20
30
0
0
C18 PHC
1
10
20
30
-4
x 10 C18:1 PHC
0
0
10
20
30
20
30
-3
C26 DHC
0.8
0
0
10
20
30
C26 PHC
1
0.8
0.015
0.6
0.01
0.4
10
0
0
C24 PHC
0.02
0.6
0
0
x 10
0.002
2
0
C16 PHC
x 10
0.012
10
0.015
8
0.4
0.005
0.2
10
20
30
0
0
10
20
30
0
0
0.2
10
20
30
0
0
10
20
30
Figure S4.1: Perturbed dataset based on 1.5 fold variation of the interpolated mean data.
1
Ceramide Synthase
Dihydroceramidase and Phytoceramidase
2
IPC Synthase
IPCase
3
Hydroxylase
Elongases 1, 2, 3, Remodelase, and Desaturase
Figure S4.2: Flux distributions calculated from perturbed data set.
4