Ecotypic variation and Seed Transfer Zones BACKGROUND While plant species can generally be treated as discrete units that have a unique ecological role relative to other species, all populations of a broadly distributed plant species cannot be assumed to be ecologically equivalent. That is, populations within a species can be grouped into ecotypes that are adapted to fit different subsets of the total niche occupied by the species. In fact, such ecoptypic differentiation seems to be the rule: locally sourced plants usually have significantly higher fitness (greater productivity, higher seed output, etc.) than conspecific plants derived from off-site [Leimu & Fischer 2008 (will there be references?)]. Furthermore, such local adaptation occurs at a spatial scale that suggests that native plant species broadly dispersed across the Colorado Plateau are comprised of distinct ecotypes. The implication for native plant restoration is that the use of locally sourced plant materials is expected to increase establishment success, and, importantly, persistence through local climatic cycles. A key question is how many seed sources need to be developed for the Colorado Plateau for a given native plant species, and over what geographic extent can each seed source be used, i.e., what is the "seed transfer zone" for that source. In this section, we will outline multiple approaches for defining native plant seed transfer zones and provide practical advice on how to use available information to ensure that the most appropriate native seed sources are used for a target site. Methods for Delineating Seed Transfer Zones Genecological Approaches including use of molecular marker data; bring discussion of generality based on mating system here, and in conclusion Reciprocal Transplant Studies most direct Climate Data Only web seed zone mapper. good start, in fact, and not surprisingly, multiple recent studies have shown the temp and precip are consistent drivers of ecolo caveats – eg doesn't include seasonality (or does it) of precip. Example map. Need to know which species are in which seed zones by filtering distribution data. Discuss and maybe insert image of monsoonal gradient to make seasonality point. (Figure 1) Conclusions Abundant data make it clear that plant species are comprised of ecologically differentiated populations, often at a fine scale. –market accommodation – summarize +/- of each empirical approach. Where to look for information for target species. Figure 1. This map of provisional seed transfer zones for the Colorado Plateau is based on average annual precipitation and maximum mean monthly temperatures. The map was produced following the strategy of the online Seedzone Mapper tool: http://www.wwetac.net/index_files/seedzone.shtml. It is important to note that nearly all restoration species occur in only a fraction of these provisional seed zones. [Example would make the point easier; have very good distr. data for BOGR2, ACHY]