PCNM1 PCNM2 PCNM3 PCNM4 PCNM5 PCNM6 6 -6 -3 0 3 Amplitude -6 -3 0 3 6 -6 -3 0 3 6 Electronic Supplementary Materials 6 1 6 12 18 24 30 34 -6 -3 0 3 PCNM7 1 6 12 18 24 30 34 Months Supplementary Figure 1 Temporal fluctuations of significant principal coordinates of neighborhood matrices (PCNM) variables selected by redundancy analyses (RDA) for the 5 sites of the Xiangxi River network 1 Supplementary Table 1 P values for pairwise comparison of Space-Time (S-T) interaction of algal relative abundance from September 2004 to June 2007 between the 5 study sites of the Xiangxi River network (P values >0.05 are underlined) xx1 xx2 xx2 jc gf gl 0.094 0.005 <0.001 <0.001 0.015 0.008 0.012 0.004 <0.001 jc gf 0.002 2 Supplementary Table 2 Percentage of taxa (%) that displayed significant correlations with specific temporal dynamics of epilithic algal assemblages from September 2004 to June 2007 at each study site of the Xiangxi River network Taxa group Site 1 2 3 4 Total xx1 16.0 8.6 xx2 22.2 8.5 5.2 jc 16.0 6.9 5.6 24.3 gf 25.7 20.6 2.2 43.4 gl 23.6 9.3 16.8 23.3 3 4.6 7.5 32.0 45.3 Supplementary Table 3 Taxa that displayed significant correlations with specific temporal dynamics of epilithic algal communities from September 2004 to June 2007 at more than 3 sites (RDA axes were used to represent specific temporal dynamics; +: taxon appeared in the taxa group) Taxa RDA1 RDA2 RDA3 RDA4 Bacillariophyceae Achnanthes lanceolata (Brébisson ex Kützing) + + + Achnanthidium affine (Grunow) Czarnecki + + Achnanthidium deflexum (Reimer) Kingston + + + + + + Cocconeis placentula Ehrenberg + + Delicata delicatula (Kützing) Krammer + Diatoma anceps (Ehrenberg) Kirchner + + Diatoma vulgaris Bory + + Encyonema alpinum (Grunow) Mann + + Encyonema minutum (Hilse) Mann + + Eunotia sp1. + + Grunow Achnanthidium minutissimum (Kützing) + Czarnecki Fragilaria capucina subsp. rumpens (Kützing) + Lange-Bertalot Fragilaria capucina var. vaucheriae (Kützing) + Lange-Bertalot 4 + + + Gomphoneis clevei (Fricke) Gil + + + Gomphonema angustatum (Kützing) Rabenhorst + + + Gomphonema parvulum (Kützing) Kützing + + Gomphonema pseudotenellum Lange-Bertalot + + Gomphonema tergestinum (Grunow) Fricke + + Grunowia tabellaria (Grunow) Rabenhorst + Humidophila Kociolek, contenta (Grunow) Johansen, Van + + Lowe, de Vijver, + + + + + Lange-Bertalot & Kopalová Navicula cryptocephala Kützing + Navicula notha Wallace + Navicula radiosafallax Lange-Bertalot + Navicula viridula (Kützing) Ehrenberg + + Nitzschia dissipata (Kützing) Rabenhorst + + Nitzschia palea (Kützing) Smith + + Pinnularia sp1. + Rhoicosphenia abbreviata (Agardh) + Lange-Bertalot Rossithidium linearis (Smith) Round & + Bukhtiyarova Stephanodiscus minutulus (Kützing) Cleve & + + Möller Synedra ulna (Nitzsch) Ehrenberg + + Tabellaria fenestrata (Lyngbye) Kützing + + Cyanobacteria 5 Lyngbya sp. + + + Oscillatoria sp. + + + Phormidium sp. + + Scenedesmus sp. + + Stigeoclonium sp. + + Ulothrix sp. + + + Chlorophyta 6 + + Supplementary Table 4 Spearman correlation coefficients between ecological guilds and physical and chemical variables (only variables with P < 0.05 listed) Physical and High profile Low profile Motile guild guild guild Site chemical variables xx1 xx2 jc Water temperature 0.387 Conductivity -0.453 Water temperature -0.444 Velocity 0.379 Conductivity -0.400 TN gf 0.383 Water temperature Conductivity gl 0.647 -0.367 0.350 Water temperature SiO2-Si -0.385 -0.429 7 0.400