3RD FAO/IAEA RESEARCH CO-ORDINATION MEETING ON "RESOLUTION OF CRYPTIC SPECIES COMPLEXES OF TEPHRITID PESTS TO OVERCOME CONSTRAINTS TO SIT APPLICATION AND INTERNATIONAL TRADE" 26 – 30 August 2013 Universidad Nacional de Tucumán, Facultad de Agronomía y Zootecnia Hotel Tucumán Center, Tucumán, ARGENTINA MONDAY, 26 AUGUST 2013 08:30 - 08:45 Teresa Vera, Jorge Hendrichs. Opening and welcome statements. Goals of the meeting, review of agenda, administrative and other issues. SESSION I : Chairperson: Gary Steck 08:45 - 09:15 Iara Sordi Joachim-Bravo, Raul Laumann, Vanessa Simões Dias, Alzira Kelly Passos Roriz, Clarissa Petitinga, Beatriz Jordão Paranhos, Aldo Malavasi. Analysis of sexual compatibility among Anastrepha fraterculus populations from Brazil. 09:15 - 09:45 J. Rull, S. Abraham, M. Mendoza, M.C. Liendo, F. Devescovi, D.F. Segura, K. Roriz, N. Nolasco, R. Castañeda, E. Tadeo, R. Brizova, A. Kovaleski and M.T. Vera. Evolution of reproductive isolation among different morphotypes of Anastrepha fraterculus. 09:45 - 10:15 Suksom Chinvinijkul, Sunyanee Srikachar, Weerawan Amornsak and Sujinda Thanaphum..Field cage mating compatibility test of Bactrocera dorsalis complex of the north and the south of Thailand. 10:15 - 10:45 Ruth R. do Nascimento, Lucie Vanicková, Adriana L. Mendonça, Alana L. Mendonça, Beatriz Jordão Paranhos, Blanka Kalinová and Antônio Euzébio Goulart Sant´Ana. Chemical studies of the sex pheromone of five Brazilian populations of Anastrepha fraterculus (Diptera: Tephritidae). 10:45 - 11:00 BREAK 11:00 - 11:30 Peter Teal. Volatile chemicals released by Tephritid flies as a tool to understanding species diversity. 11:30 - 12:00 Radka Břízová, Lucie Vaníčková, Blanka Kalinová, Michal Hoskovec, Ruth Rufino Do Nascimento. Pheromones as an indispensible instruments for distiction of Anastrepha fraterculus by age and geographic origin. Document1 Page 2 12:00 - 12:30 Suk-Ling Wee, Alvin Kah-Wei Hee and Keng-Hong Tan. Emission of male sex pheromone by four cryptic species, Bactrocera dorsalis, B. invadens, B. papayae and B. philippinensis, following Methyl Eugenol consumption. 12:30 - 13:00 Blanka Kalinová, Radka Břízová, Maria Faťarová, Lucie Vaníčková, Michal Hoskovec. Pheromones of three species of Ceratitis FAR complex. 13:00 - 14:00 LUNCH SESSION II : Chairperson: Sunday Ekesi 14:00 - 14:30 Lucie Vaníčková, Massimiliano Virgilio, Aleš Tomčala, Radka Břízová, Adriana de Lima Mendonça, Blanka Kalinová, Ruth Rufino Do Nascimento, and Marc De Meyer. Cuticular hydrocarbons as a tool for resolution of the fruit fly species complexes. 14:30 - 15:00 Ishan ul Haq, Marc J.B. Vreysen, Todd E. Shelly, Jorge Hendrichs. Methyl eugenol sensitivity and its effects on intraspecific mating of three sibling species of the Bactrocera dorsalis complex. 15:00 - 15:30 Alvin Kah-Wei Hee, Suk-Ling Wee and Keng-Hong Tan. Comparative sensitivity of males of four globally important cryptic species in the Bactrocera dorsalis complex - B. dorsalis, B. invadens, B. papayae and B. philippinensis, to Methyl Eugenol and subsequent male antennal protein profiles. 15:30 - 15:45 BREAK 15:45 - 16:15 Elena Drosopoulou, A. Augustinos, A. Gariou-Papalexiou, C. CaceresBarrios, K. Bourtzis, P. Mavragani-Tsipidou, A. Zacharopoulou. Cytogenetic studies in members of Bactrocera dorsalis species complex: A comparative analysis. 16:15 - 16:45 Nelson A. Canal, Julio C. Carranza, Daniel Zabala, María del Rosario Castañeda. Population structure of the Andean morphotype of the Anastrepha fraterculus complex. 16:45 - 17:15 María del Rosario Castañeda, Daniel Zabala, F. M. Patarroyo, Julio C. Carranza, Nelson A. Canal. Population studies of Anastrepha obliqua (MacQuart, 1835). 17:15 - 17:45 General Discussion Page 3 TUESDAY, 27 AUGUST 2013 08:30 - 08:45 Teresa Vera, Jorge Hendrichs. Announcements, adjustments to the agenda, administrative and other issues. SESSION III : Chairperson: Blanka Kalinova 08:45 - 09:15 Janisete G. Silva, Katia M. Lima, Norman B. Barr, Raul Ruíz-Arce, Gary J. Steck, Bruce. A. McPheron, Roberto A. Zucchi. Use of mtDNA and nuclear markers to delimit and diagnose Brazilian species within the Anastrepha fraterculus complex. 09:15 - 09:45 L. Paulin, P. Gómez Cendra, M. Juri, S. Lanzavecchia, M. T. Vera, J. Cladera, J. C. Vilardi. Landscape genetics approach to study Anastrepha fraterculus populations. 09:45 - 10:15 Karen F. Armstrong, L. M. Boykin, A. Chomic, M. K. Schutze, A. R. Clarke. Molecular genetic resolution of pest species within the Bactrocera dorsalis complex 10:15 - 10:45 Anna R Malacrida, Nidchaya Akeratawong, Silvia Lanzavecchia, Blanka Kalinova, Giuliano Gasperi. Looking for species-specific sequences in Bactrocera, Anastrepha and Ceratitis species. 10:45 - 11:00 BREAK 11:00 - 11:30 Qinge Ji. Bactrocera dorsalis complex species in mainland China. 11:30 - 12:00 Nidchaya Aketarawong, Siriwan Isasawin, Anna R. Malacrida, Giuliano Gasperi, Sujinda Thanaphum. Evaluation of genetic sexing strain, Bactrocera dorsalis Salaya1 for its species complex members and molecular characterization of Y-chromosome sequences of Bactrocera dorsalis. 12:00 - 12:30 Sunday Ekesi, Chrysantus M. Tanga, Fathiya M. Khamis and Samira A. Mohamed. Host plant relationships, temperature tolerance and mating compatibility studies in Bactrocera cucurbitae and Ceratitis rosa. 12:30 - 13:00 Maulid W. Mwatawala, Massimo Virgilio, Marc De Meyer. Comparative studies on behaviour and developmental physiology of Ceratitis rosa entities (R1 and R2) occurring in Tanzania. 13:00 - 14:00 LUNCH SESSION IV : Chairperson: Peter Teal Page 4 14:00 - 14:30 Vicente Hernández-Ortiz, Nelson A. Canal and Juan O. Tigrero. Morphometrics of wild populations from Ecuador and Colombia of the Anastrepha fraterculus complex: The eighth passenger. 14:30 - 15:00 Gary Steck. Anastrepha species of the Central Andes of Peru. 15:00 - 15:30 M. K. Schutze and A. R. Clarke. The taxonomic history of the Oriental and invasive fruit fly: from Musca ferruginea Fabricius to Bactrocera invadens Drew, Tsuruta & White. 15:30 - 15:45 BREAK 15:45 - 16:15 M. K. Schutze, S.L. Cameron, K. Mahmood, W. Bo, C. Cáceres, M.J.B. Vreysen, A. Pavasovic, A. R. Clarke. Reproductive compatibility, morphological and morphometric variation, and molecular genetic analyses of Bactrocera dorsalis sensu lato (Diptera: Tephritidae) from Africa, the Indian Subcontinent and East Asia. 16:15 - 16:45 J. Van Cann, M. Virgilio, K. Jordaens and M. De Meyer. The Ceratitis FAR complex: biogeography, host plant spectrum and morphometrics. 16:45 - 17:30 General Discussion and organization of three Working Groups: a) Anastrepha fraterculus complex, b) Bactrocera dorsalis complex, and c) Bactrocera cucurbitae & Ceratitis FAR complex. 19:00 - 22:00 Social Event WEDNESDAY, 28 AUGUST 2013 SESSION V : Chairpersons Jorge Hendrichs and Group Leaders 08:30 - 10:30 Working Groups. Review of achievements based on original coordinated research plans and drafting of results and scientific/technical conclusions for the different genera, approaches and techniques. Review of research needs to be addressed and other activities to be implemented. 10:30 - 10:45 BREAK 10:30 - 13:00 Working Groups – continued 13:00 - 14:00 LUNCH 14:00 - 15:30 Working Groups – continued 15:30 - 15:45 BREAK Page 5 15:45 - 17:30 Working Groups Presentations – Review of achievements, conclusions and recommendations on research needs and other activities for the different genera, approaches and techniques. THURSDAY, 29 AUGUST 2013 SESSION VI : Chairpersons Jorge Hendrichs and Group Leaders 08:30 - 10:30 Working Groups – Drafting of individual research proposals for the different genera and techniques, taking into account the proposed R&D of each research team. 10:30 - 10:45 BREAK 10:45 - 13:00 Working Groups – continued 13:00 - 14:00 LUNCH 14:00 - 15:30 Working Group Presentations – Review of recommendations for the individual research proposals for the different applications of each research team. 15:30 - 15:45 BREAK 15:45 - 17:00 General Discussion – Organization of the preparation of final CRP proceedings in a special issue and potential journals to be contacted. FRIDAY, 30 AUGUST 2013 SESSION VII : Chairpersons Jorge Hendrichs and Group Leaders 08:30 - 10:30 Working Groups – Drafting and compiling of RCM report. 10:30 - 10:45 BREAK 10:45 - 13:00 Working Group – continued 13:00 - 14:00 LUNCH 14:00 - 16:30 Working Groups – General Discussion: Agreement on content of RCM report, on information exchange mechanisms, on location of Final RCM and closure of the RCM. Page 6