Dear Researcher:
You have received a series of inducible lentiviral gene silencing vectors and gene
expression vectors. All of the vectors for inducible gene silencing (pInducer10’s)
should be grown in LB with 100 ug/ml of ampicillin. They all have a PheS Gly294
gene (1.4 kb) between XhoI and EcoRI. You can replace the PheS Gly294 gene with a
mir30 based hairpin of your interest. All of the vectors for inducible gene
expression (pInducer20’s) should be grown in LB with 50 ug/ml of ampicillin and
30 ug/ml of chloramphenicol. I found it is easier to grow the gene expression
vectors at 30C. All of the vectors are already in E.coli. Upon receipt, you should lay
the filter paper on LB plate containing appropriate antibiotics and grow overnight,
then streak for single colony the next day. If you have any question, feel free to
contact me (mli@rics.bwh.harvard.edu).
Sincerely,
Mamie Li