LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – BIO TECHNOLOGY
SECOND SEMESTER – APRIL 2006
BT 2813 - GENETIC ENGINEERING
Date & Time : 19-04-2006/FORENOON
Dept. No.
Max. : 100 Marks
Part -A
Answer all the questions:
I. Choose the best answer
(20 marks)
(5 x 1 = 5)
1. Which one of the following cut methylated DNA?
a) Bam HI
b) EcoRI
c) TaqI
d) McrBC
2. …………. reduces gap in physical map
a) macrorestriction
b) microrestriction
c) RH
d) cell panel
3. Multiple copy insertion is predominant in ………….
a) Agrobacterium transformation
b) Biolistic
c) Microinjection
d) protoplast fusion
4. …………. Tool is based on the conformational change.
a) SNP
b) SSR
c) SSCP
d) EST
5. ……. is a selectable marker
a) GUS
b) GAL
c) CAT
d) NPT II
II State true or False. Correct the statement if false
(5 x 1 = 5)
6. Restriction enzymes with same single stranded overhang region is isoschizomers.
7. Insertional mutagenesis results in inactivation of endogenous gene.
8. The capacity of the YAC vector is 50 Kb.
9. In RAPD two primers are used.
10. Coat protein mediated resistance is plant borne mechanism
III. Complete the following
(5 x 1 = 5)
11. Relaxed specificity of cutting by restriction enzyme is known as …………
12. South - western is used to study ……….
13. Transgene silencing by multiple copy is due to …………
14. Homology in ……….. region results in transcriptional gene silencing
15. In tissue culture virus resistance plants are produced by ……..
IV. Answer the following not exceeding 50 words each.
Define the following:
(5 x 1 =5)
16. Selectable and scorable markers.
17. Contigs.
18. Conjugation.
19. Gene pyramids.
20. Physical Containment.
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Part -B
Answer any FIVE of the following not exceeding 350 words each.
(5 x 8 = 40)
21. Comment on the properties of phage vectors.
22. Give the physical map and the importance of pBR322.
23. What is colony hybridization? Explain how it is done.
24. Explain differential screening and subtractive hybridization with diagrams.
25. Describe gene introduction into cells by microinjection.
26. What is a probe? What are the types of probes? Explain Stringency.
27. What is foot printing? Explain.
28. What is cloning? Describe mononuclear reproduction.
Part - C
Answer the following not exceeding 1500 words each.
(2 x 20 = 40)
29. a) Describe the construction of shuttle vectors.
b) What is physical mapping? How do you construct a physical map?
Or
a) Write notes on adopters, linkers and homopolymer tails.
b) Explain Agrobacterium mediated transformation in plant cells.
30. a) Describe the methods of transcript mapping with diagrams
b)What are the types of yeast vectors? Describe yeast integrative vector.
Or
a) Describe the approaches to create herbicide resistance in plants.
b) Discuss the role of recombinant DNA in medicine.
____________
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