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Author 1 , Author 2
* (Cordia New, 14pt, bold, Center)
1 Department of ................., Faculty of Medical Science, Phitsanulok,
Thailand 65000
2 Department of ................., Faculty of Medical Science, Phitsanulok,
Thailand 65000
(Cordia New, 14pt, Italic, Center)
*
Corresponding author;
* E-mail: Corresponding author (Cordia New, 12pt,
Regular, Center)
Abstract (Cordia New, 14pt, bold)
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(Cordia New, 14pt, single spacing no more than 300 words)
Key words: (Cordia New, 14pt, Regular no more than 5 words )
Medical Science Academic Annual Meeting 2012, 5-6 th March 2012, Faculty of Medical
Science, Naresuan U., Phitsanulok / 1

Walailuk Kerdsan 1 , Samur Thanoi 1 , Paritat Watiktinkorn 2 , Gavin P.
Reynolds 3 , Sutisa Nudmamud-Thanoi 1
*
1 Department of Anatomy, Faculty of Medical Science, Phitsanulok, Thailand
65000
2 Synphaet hospital, Bangkok, Thailand 10230
3 Biomedical Research Centre, Sheffield Hallam University , Sheffield S10
4LN U.K.
Medical Science Academic Annual Meeting 2012, 5-6 th March 2012, Faculty of Medical
Science, Naresuan U., Phitsanulok / 2

*
Corresponding author;
*
E-mail: sutisat@nu.ac.th
Abstract
Research strongly implicates glutamatergic system in etiology of drug dependence. Changes in glutamate transporter EAAT2 protein expression have been reported after exposure to methamphetamine and cocaine.
EAAT2 gene variant have been associated with schizophrenia, some positive symptoms are similar to those occurring in methamphetamine dependence.
Agonist to glutamate receptor GRM3 has been reported to inhibit amphetamine-induced behavioral and cognitive abnormalities. Moreover, polymorphism of the GRM3 gene has been associated with a low of EAAT2 mRNA level and an impairment of cognitive function in schizophrenia.
Therefore, the aim of this study was to investigate the association of EAAT2 and GRM3 gene polymorphisms with methamphetamine dependence. One hundred methamphetamine dependence, meeting DSM-IV criteria, and 102 ethnically-matched healthy subjects were genotyped for EAAT2 (rs1885343) gene and GRM3 (rs6465084) gene polymorphisms from blood using PCR-
RFLP and real-time PCR methods, respectively. The genotype and allele frequencies were analyzed using Chi-square and Fisher’s exact tests. The polymorphisms for both control and methamphetamine groups were in
Hardy-Weinberg equilibrium. There were no significant difference between groups in either genotype (P=0.117) or allele frequencies (P=0.228) in rs1885343. Additionally, a significant excess of G/G genotype versus A allele carriers was also observed in rs1885343 in methamphetamine group relative to control group (P=0.010). For the rs6465084, statistical differences between the groups were not detected in either genotype (P=0.951) or allele frequencies (P=0.777). The results revealed an association between EAAT2 gene polymorphism and methamphetamine dependence. This finding is
Medical Science Academic Annual Meeting 2012, 5-6 th March 2012, Faculty of Medical
Science, Naresuan U., Phitsanulok / 3

consistent with a study found an association of the EAAT2 polymorphism with schizophrenia. Moreover, genetic variation of the EAAT2 gene has been contributed to alcoholic behavior. Our findings suggest that polymorphism of the EAAT2 gene, but not the GRM3 gene may confer vulnerability to a risk factor for methamphetamine dependence.
Key words: Methamphetamine, Glutamate, EAAT2 gene, GRM3 gene,
Polymorphism
Medical Science Academic Annual Meeting 2012, 5-6 th March 2012, Faculty of Medical
Science, Naresuan U., Phitsanulok / 4