Supplemental Materials for:
Effects of environmental manipulations and treatment with bupropion and risperidone
on choice between methamphetamine and food in rhesus monkeys
Matthew L. Banks1,2,* and Bruce E. Blough3
1
Department of Pharmacology and Toxicology, Virginia Commonwealth University, Richmond,
VA USA
2
3
Institute for Drug and Alcohol Studies, Virginia Commonwealth University
Center for Organic and Medicinal Chemistry, Research Triangle Institute, Research Triangle Park, NC
USA
Banks Supplemental 2
Table 1: Mean methamphetamine ED50 value in mg/kg/injection (95% confidence limits) under
+ Saline (Baseline) and during 7-day treatment with bupropion and risperidone.
Treatment Condition
ED50 (95% confidence limit)
+ Saline (n=6)
0.088 (0.063-0.125)
Bupropion, 0.32 mg/kg/h (n=4)
0.099 (0.065-0.153)
Bupropion, 1.0 mg/kg/h (n=4)
0.096 (0.059-0.156)
Bupropion, 1.8 mg/kg/h (n=4)
0.143 (0.093-0.218) a
Risperidone, 0.001 mg/kg/h (n=3)
0.141 (0.106-0.187)
Risperidone, 0.0032 mg/kg/h (n=3)
0.054 (0.033-0.089)
Risperidone, 0.0056 mg/kg/h (n=3)
Could not be determined b
a
Mean ED50 value could not be determined because methamphetamine choice was not greater
than 50% at any methamphetamine dose for some components and responding was eliminated
during other components of the choice session in one monkey.
b
Mean ED50 value could not be determined because methamphetamine choice was 100% for
some components and responding was eliminated during other components of the choice session
in one monkey and methamphetamine choice was not lower than 50% at any methamphetamine
does for two monkeys.
Banks Supplemental 3
Figure 1: Effects of continuous bupropion (0.32 – 1.8 mg/kg/h) treatment on choice between
methamphetamine and food in rhesus monkeys (n=4). Top panel abscissae: unit dose
methamphetamine in mg/kg/injection (log scale). (a) Left ordinate: percent methamphetamine
choice. Right ordinate: percent food choice. Bottom panel shows summary data for total choices,
food choices, and methamphetamine choices summed across all methamphetamine doses.
Ordinate: number of choices per session. Abscissa: experimental endpoint. All points and bars
represent mean  SEM obtained during days 1-3 of each 7-day treatment period.
Banks Supplemental 4
Figure 2: Effects of continuous risperidone (0.001 – 0.0056 mg/kg/h) treatment on choice
between methamphetamine and food in rhesus monkeys (n=3). All points and bars represent
mean  SEM obtained during days 1-3 of each 7-day treatment period. Other details are same as
in Figure 1.
Banks Supplemental 5
Figure 3: Effects of removing methamphetamine or food availability on behavioral allocation in
rhesus monkeys (n=5). Top panel abscissae: unit dose methamphetamine in mg/kg/injection (log
scale) during + saline and ‘no food’ conditions, or unit dose methamphetamine associated with
prevailing discriminative stimuli during the ‘no methamphetamine’ condition. (a) Left ordinate:
percent methamphetamine choice or methamphetamine-associated stimuli. Right ordinate:
percent food choice or food-associated stimuli. All points and bars represent mean  SEM
obtained during days 1-3 of each 7-day treatment period. Filled symbols indicate significantly
different (p < 0.05) from baseline (+ saline) within a methamphetamine dose. Asterisk indicates
significantly different from baseline (+ saline) conditions. Removing food availability
Banks Supplemental 6
significantly increased responding on the methamphetamine-associated key when 0.032
mg/kg/injection methamphetamine was available (Panel A; reinforcer availability: F2,44=3.5,
p=0.038) and significantly decreased the number of choices completed on the food-associated
key (Panel B; reinforcer availability  dependent measure: F4,32=7.3, p=0.0003).