Appendix S1. Food web information Table S1.1. List of taxa. Taxon ID Order Family Subfamily Genus Epithet 1 Amphipoda Gammaridae NA Gammarus near tigrinusα 2 Lumbriculida Lumbriculidae NA Gen spβ 3 Anura Hylidae NA Acris gryllusγ 4 Anura Ranidae NA Rana sphenocephalaγ 5 Calanoida Fam NA Gen spδ 6 Coleoptera Dytiscidae NA Bidessonotus spε 7 Coleoptera Dytiscidae NA Copelatus sp larvaeζ 8 Coleoptera Dytiscidae NA Copelatus caelatipennisε 9 Coleoptera Dytiscidae NA Hydaticus sp larvaeζ 10 Coleoptera Dytiscidae NA Hydaticus bimarginatusε 11 Coleoptera Dytiscidae NA Hydroporus spε 12 Coleoptera Dytiscidae NA Ilybius oblitusε 13 Coleoptera Dytiscidae NA Gen spε 14 Coleoptera Dytiscidae NA Rhantus calidusε 15 Coleoptera Dytiscidae NA Thermonectus basilarisε 16 Coleoptera Hydrophilidae NA Berosus ordinatusε 17 Coleoptera Hydrophilidae NA Berosus striatusε 18 Coleoptera Hydrophilidae NA Paracymus confususε 19 Coleoptera Hydrophylidae NA Berosus exiguousε 20 Coleoptera Hydrophylidae NA Gen sp larvaeζ 21 Coleoptera Noteridae NA Notomucurus annulusε 22 Coleoptera Noteridae NA Suphisellus spε 23 Poduromorpha Neanuridae NA Gen spδ 24 Cyclopoida Cyclopidae NA Acanthocyclops spδ 25 Cyclopoida Cyclopidae NA Eucyclops spδ 26 Cyclopoida Cyclopidae NA Eucyclops elegansδ 27 Cyclopoida Cyclopidae NA Mesocyclops edaxδ 28 Cyclopoida Cyclopidae NA Microcyclops sp aδ 29 Cyclopoida Cyclopidae NA Microcyclops sp bδ 30 Cyclopoida Cyclopidae NA Microcyclops sp cδ 31 Cyclopoida Cyclopidae NA Nauplius spδ 32 Cyclopoida Cyclopidae NA Paracyclops spδ 33 Cyclopoida Cyclopidae NA Tropocyclops spδ 34 Cyclopoida Cyclopidae NA Tropocyclops prasinusδ 35 Diplostraca Bosminidae NA Bosmina longirostrisη 36 Diplostraca Chydoridae NA Alona spη 37 Diplostraca Chydoridae NA Chydorus sphaericusη 38 Diplostraca Daphnidae NA Cereodaphnia reticulataη 39 Diplostraca Daphniidae NA Daphnia ambiguaη 40 Diplostraca Macrothricidae NA Grimaldina spη 41 Diplostraca Macrothricidae NA Ilyocryptus spiniferη 42 Diplostraca Macrothricidae NA Kurtzia latissimaη 43 Diplostraca Macrothricidae NA Lathonura rectirostrisη 44 Diplostraca Macrothricidae NA Macrothrix roseaη 45 Diplostraca Macrothricidae NA Macrothrix spη 46 Diplostraca Macrothricidae NA Macrothrix laticornisη 47 Diplostraca Macrothricidae NA Gen spη 48 Diplostraca Macrothricidae NA Streblocercus spη 49 Diplostraca Moinidae NA Moina spη 50 Diplostraca Fam NA Gen spη 51 Diplostraca Daphniidae NA Scapholebris mucronataη 52 Diplostraca Sididae NA Diaphanasoma brachyurumη 53 Diptera Chaoboridae NA Chaoborus punctipennisθ 54 Diptera Chironomidae NA Ablabesmyia mallochiι 55 Diptera Chironomidae Chironominae Chironomus spι 56 Diptera Chironomidae Chironominae Chironomus near decorusι 57 Diptera Chironomidae Chironominae Chironomus ocreatusι 58 Diptera Chironomidae Chironominae Chironomus spι 59 Diptera Chironomidae Chironominae Chironomus decurus groupι 60 Diptera Chironomidae Chironominae Glyptotendipes spι 61 Diptera Chironomidae Chironominae Goeldichironomus spι 62 Diptera Chironomidae Chironominae Goeldichironomus holoprasinusι 63 Diptera Chironomidae Chironominae Goeldichironomus cf natansι 64 Diptera Chironomidae Chironominae Kiefferulus spι 65 Diptera Chironomidae Chironominae Kiefferulus dux pungensι 66 Diptera Chironomidae Tanypodinae Gen spι 67 Diptera Chironomidae NA Gen spι 68 Diptera Chironomidae Chironominae Gen spι 69 Diptera Chironomidae Chironominae Paratanytarsus spι 70 Diptera Chironomidae Chironominae Polypedilum tritumι 71 Diptera Chironomidae Chironominae Polypedilum cf convictumι 72 Diptera Chironomidae Chironominae Polypedilum trigonusι 73 Diptera Chironomidae Tanypodinae Tanypus punctipennisι 74 Diptera Chironomidae Chironominae Tanytarsus spι 75 Coleoptera Noteridae NA Gen spε 76 Diptera Culicidae NA Aedes spκ 77 Diptera Culicidae NA Aedes aegyptiκ 78 Diptera Culicidae NA Aedes vexansκ 79 Diptera Culicidae NA Anopheles spκ 80 Diptera Culicidae NA Anopheles cruciansκ 81 Diptera Culicidae NA Culex spκ 82 Diptera Culicidae NA Culex nigripalpusκ 83 Diptera Culicidae NA Culex salinariusκ 84 Diptera Culicidae NA Gen spκ 85 Diptera Culicidae NA Psorophora spκ 86 Diptera Culicidae NA Psorophora feroxκ 87 Diptera Tabanidae NA Gen spκ 88 Diptera Tabanidae NA Gen spκ 89 Diptera Tabanidae NA Tabanus spκ 90 Diptera Tipulidae NA Tipula NAκ 91 Ephemeroptera Baetidae NA Callibaetis spλ 92 Ephemeroptera Baetidae NA Callibaetis floridanusλ 93 Ephemeroptera Baetidae NA Gen spλ 94 Heteroptera Coryxidae NA Sigara spμ 95 Heteroptera Gerridae NA Limnopus canaliculatusρ 96 Heteroptera Notonectidae NA Notonecta undulataμ 97 Heteroptera Veliidae NA Microvelia sp aν 98 Heteroptera Veliidae NA Microvelia albonataν 99 Heteroptera Veliidae NA Microvelia sp bν 100 Hirudinida Hirudinidae NA Gen spξ 101 Microsporales Microsporaceae NA Microspora spο 102 Trombidiformes Hydracarinidiae NA Gen spπ 103 Orobatida Fam NA Gen spπ 104 Trichurida Capillariidae NA Gen spι 105 Odonata Libellulidae NA Libellula auripennisς 106 Odonata Libellulidae NA Libellula spς 107 Odonata Libellulidae NA Orthemis ferrugineaς 108 Odonata Libellulidae NA Pantala flavescensς 109 Odonata Libellulidae NA Gen spς 110 Ostracoda Fam NA Gen spς 111 Ploima Asplanchinidae NA Harringia spσ 112 Ploima Brachionidae NA Brachionus spσ 113 Ploima Brachionidae NA Brachionus quadridentataσ 114 Ploima Brachionidae NA Brachionus rubensσ 115 Ploima Brachionidae NA Platyias quadricornisσ 116 Ploima Euchlanidae NA Euchlanis spσ 117 Ploima Lecanidae NA Lecane spσ 118 Ploima Lecanidae NA Lecane ungulataσ 119 Ploima Lecanidae NA Monostyla spσ 120 Ploima Lecanidae NA Monostyla quadridentataσ 121 Ploima Lepadellidae NA Lepadella spσ 122 Ploima Lecanidae NA Gen spσ 123 Ploima Notommatidae NA Cephalodella spσ 124 Ploima Notommatidae NA Monommata spσ 125 Ploima Notommatidae NA Notommata spσ 126 Ploima Trichocercidae NA Trichocerca spσ 127 Ploima Trichocercidae NA Trichocerca bicristataσ 128 Tricladida Planariidae NA Planaria NAτ 129 Ulotrichales Ulotrichaceae NA Ulothrix spο 130 Volvocales Volvocaceae NA Eudorina spο 131 Zygnematales Desmidiaceae NA Closterium spο 132 Zygnematales Desmidiaceae NA Micrasterias radiataο 133 Zygnematales Desmidiaceae NA Gen spο 134 Zygnematales Desmidiaceae NA Staurastrum arctisconο 135 Zygnematales Zygnemataceae NA Mougeotia spο 136 Zygnematales Zygnemataceae NA Gen spο 137 Zygnematales Zygnemataceae NA Spirogyra spο 138 Detritus NA NA NA NA Body Length-Mass Conversions Symbol Length-Mass Conversion Source α M= β M = 5.61L2.709 γ M= 0.006L3.24 Pough (1980) Am Nat 115: 92-112. M= 0.00281L2.606 Culver et al. (1985) Can J Fish & Aq Sci 42: 1380-1390. M= 0.0618L2.502 Benke et al. (1999) J N. Amer Benth Soc: 308-343. M= 0.0181L2.311 η M= 0.00466459L2.29 θ M = 0.000453L2.43 Benke et al. (1999) J N. Amer Benth Soc: 308-343. McCauley (1984) A manual on methods of assessment for secondary productivity in fresh waters 17: 228-265. Johnson & Cunjak (1999) Freshwater Biology 41: 653-674. ι M = 0.0018L2.617 Benke et al. (1999) J N. Amer Benth Soc: 308-343. δ ε ζ κ 0.012L2.74 Benke et al. (1999) J N. Amer Benth Soc 18: 308-343. Lindegaard (1992) Oikos 64: 257-304. M= 0.032L2.038 Sabo et al. (2002) J N. Amer Benth Soc 21: 336-343. λ M= 0.00081L3.574 Benke et al. (1999) J N. Amer Benth Soc: 308-343. μ M = 0.0031L2.904 Benke et al. (1999) J N. Amer Benth Soc: 308-343. ν M= 0.0126L2.719 Benke et al. (1999) J N. Amer Benth Soc: 308-343. ξ M= 2.465L3.886 ο see Table 7.5 π M = 0.04L3 Benke et al. (1999) J N. Amer Benth Soc: 308-343. McCauley (1984) A manual on methods of assessment for secondary productivity in fresh waters 17: 228-265. Benke et al. (1999) J N. Amer Benth Soc: 308-343. ρ M = 0.015L2.596 Benke et al. (1999) J N. Amer Benth Soc: 308-343. ς M = 0.0076L2.809 σ see Table 7.5 τ M = 0.0082L2.168 Benke et al. (1999) J N. Amer Benth Soc: 308-343. McCauley (1984) A manual on methods of assessment for secondary productivity in fresh waters 17: 228-265. Benke et al. (1999) J N. Amer Benth Soc: 308-343. Table S1.2. Consumer-Resource relationships and diet sources. Consumer ID 1 A,B 2 A,B Resource IDs 1 31 32 41 131 138 130 131 132 134 138 3 C 138 4 C 131 132 134 5 B,D 5 23 24 25 29 31 35 36 37 39 41 51 52 110 113 116 117 119 121 123 130 131 132 138 6 A,D,E,F,G,H 7 A,D,E,F,G,H 8 A,D,E,F,G,H 9 A,D,E,F,G,H 10 A,D,E,F,G,H 11 A,D,E,F,G,H 12 A,D,E,F,G,H 6 67 81 84 97 7 23 24 25 31 67 68 81 82 83 84 97 2 5 8 23 24 25 27 29 30 31 34 35 36 37 38 39 41 42 44 45 46 47 48 49 50 52 53 54 55 56 59 62 64 65 66 67 68 70 74 76 77 78 79 80 81 82 84 87 89 92 97 99 9 10 13 18 23 27 29 30 31 32 37 38 44 46 50 53 64 65 67 82 85 87 93 95 100 105 106 107 2 4 5 8 9 10 11 13 15 16 17 19 21 22 23 24 25 26 27 29 30 31 32 33 34 35 36 37 38 39 41 42 44 45 46 47 48 49 50 51 52 53 54 55 56 58 59 60 62 63 64 65 67 68 70 71 76 77 78 79 81 82 84 85 86 87 89 90 94 95 96 104 105 106 108 109 2 23 27 31 34 37 41 42 70 81 97 98 14 A,D,E,F,G,H 8 31 34 50 2 13 23 24 27 29 30 31 32 33 34 37 44 46 50 53 55 62 64 65 67 68 74 75 76 79 81 82 84 87 93 97 99 100 14 31 34 37 38 59 67 81 92 97 15 A,D,E,F,G,H 8 24 31 38 53 62 81 97 13 A,D,E,F,G,H 16 A,D,E,F,G,H 8 31 44 49 81 82 97 130 17 A,D,E,F,G,H 13 30 44 55 82 97 130 132 138 18 A,D,E,F,G,H 13 27 29 30 32 37 44 46 64 65 87 93 100 131 132 19 A,D,E,F,G,H 8 23 48 67 68 78 97 138 20 A,D,E,F,G,H 44 114 21 A,D,E,F,G,H 8 24 31 38 53 62 81 97 22 A,D,E,F,G,H 23 I 64 67 86 130 131 132 138 30 D,E,J 138 2 23 24 31 34 36 37 41 42 44 45 50 51 52 55 58 59 62 63 64 65 67 68 70 81 82 83 84 87 104 111 112 113 114 115 116 117 119 120 121 123 126 127 128 2 23 24 29 31 34 36 37 41 42 44 46 50 51 52 55 58 59 60 61 62 64 65 67 68 70 71 81 82 83 84 87 104 111 112 113 114 116 117 119 120 121 123 126 127 2 31 52 62 65 71 82 2 23 24 29 30 31 34 36 37 41 42 44 46 47 48 50 52 59 64 65 67 68 70 81 82 84 87 104 111 112 113 114 115 116 117 118 119 120 121 122 123 124 127 2 23 29 34 50 65 67 84 117 119 123 2 23 29 30 31 33 34 36 37 40 41 43 44 46 47 48 50 51 52 55 57 58 60 64 65 67 68 72 81 82 83 84 87 104 112 113 114 116 117 118 119 120 121 122 123 124 23 29 30 31 36 37 44 46 55 64 65 82 87 113 116 117 118 119 121 31 D,E,J 2 81 111 116 117 119 120 121 122 123 124 125 126 127 128 130 131 132 134 138 24 D,E,J 25 D,E,J 26 D,E,J 27 D,E,J 28 D,E,J 29 D,E,J 32 D,E,J 29 34 41 46 63 64 65 70 87 116 117 118 120 121 131 132 138 33 D,E,J 34 D,E,J 29 53 87 116 120 121 130 131 2 28 29 34 38 41 42 46 50 52 53 55 59 63 64 65 67 68 70 87 89 92 104 116 117 118 119 120 121 122 123 124 125 127 130 131 132 138 130 131 132 138 35 D,E,F 36 D,E,F 130 131 132 138 37 D,E,F 130 131 132 134 138 38 D,E,F 131 132 138 39 D,E,F 130 131 132 138 40 D,E,F 130 131 138 41 D,E,F 130 131 132 134 138 42 D,E,F 130 131 132 138 43 D,E,F 130 138 44 D,E,F 130 131 132 134 138 45 D,E,F 131 132 138 46 D,E,F 130 131 132 134 138 47 D,E,F 130 131 132 138 48 D,E,F 138 49 D,E,F 130 138 50 D,E,F 130 131 132 134 138 51 D,E,F 52 D,E,F 130 131 132 138 54 A,B,E,F,J 130 131 132 134 138 5 53 55 59 62 64 67 68 79 81 82 84 85 87 89 112 113 114 116 117 119 120 121 123 124 127 128 2 54 130 131 132 138 56 A,B,E,F,J 130 138 53 A,B,F,J 57 A,B,E,F,J 130 131 138 58 A,B,E,F,J 130 131 138 59 A,B,E,F,J 131 132 138 60 A,B,E,F,J 130 131 132 138 61 A,B,E,F,J 138 62 A,B,E,F,J 131 138 63 A,B,E,F,J 131 132 138 64 A,B,E,F,J 130 131 132 138 65 A,B,E,F,J 130 131 132 138 66 A,B,E,F,J 132 138 67 A,B,E,F,J 130 131 132 134 138 68 A,B,E,F,J 130 131 132 134 138 69 A,B,E,F,J 138 70 A,B,E,F,J 130 131 132 138 71 A,B,E,F,J 131 138 72 A,B,E,F,J 130 131 138 74 A,B,E,F,J 130 131 138 75 A,D,E,F,G,H,J 23 62 84 100 76 A,J 130 131 132 134 138 77 A,J 130 131 132 134 138 78 A,J 138 79 A,J 131 138 80 A,J 131 132 138 81 A,J 130 131 132 134 138 82 A,J 130 131 132 134 138 83 A,J 131 138 84 A,J 130 131 132 134 138 85 A,J 131 132 138 86 A,J 130 131 132 138 87 A,J 130 131 132 134 138 88 A,J 130 138 89 A,J 131 132 134 138 90 A,J 130 131 132 138 91 A,J 138 92 A,J 131 138 93 A,J 131 132 138 94 A,K 131 132 138 95 A,K 23 24 25 27 29 30 31 32 34 37 44 46 53 64 65 79 82 84 87 96 A,K 2 31 34 52 65 84 87 94 108 110 97 A,K 65 76 77 78 79 80 81 82 83 84 85 86 87 98 A,K 81 99 A,K 104 J 76 79 81 82 110 B,J 2 8 10 14 19 105 108 109 2 3 5 7 8 13 14 18 23 24 25 27 29 30 31 32 34 35 37 38 39 40 41 44 46 50 52 53 55 59 64 65 67 68 72 77 79 81 82 84 87 92 93 99 105 106 107 109 110 112 113 2 5 8 13 18 22 23 24 25 27 29 30 31 32 33 34 35 36 37 38 39 41 44 46 50 51 52 53 60 62 64 65 67 68 74 75 76 81 82 84 86 87 89 90 93 99 105 106 107 109 110 113 114 13 18 23 27 29 30 32 37 44 46 64 65 74 87 88 93 105 106 107 113 2 8 23 25 28 29 31 34 37 41 44 50 52 55 57 58 61 62 63 65 67 68 74 80 81 82 84 87 94 108 109 110 113 2 3 7 8 13 14 15 16 17 21 23 24 25 27 29 30 31 33 34 36 37 38 44 46 49 52 53 55 59 62 64 65 67 68 70 76 78 79 80 81 82 84 89 92 94 99 105 106 108 109 110 113 138 111 D,J 131 132 138 105 A,B,E,J 106 A,B,E,J 107 A,B,E,J 108 A,B,E,J 109 A,B,E,J 112 D,J 130 131 132 134 138 113 D,J 130 131 132 134 138 114 D,J 130 131 132 138 115 D,J 131 138 116 D,J 130 131 132 134 138 117 D,J 130 131 132 134 138 118 D,J 130 131 132 138 119 D,J 130 131 132 134 138 120 D,J 130 131 132 134 138 121 D,J 130 131 132 134 138 122 D,J 131 138 123 D,J 124 D,J 130 131 132 134 138 130 131 134 138 125 D,J 131 131 138 126 D,J 130 131 132 134 138 127 D,J 130 131 132 134 138 128 A,B 119 121 131 Diet Sources A Voshell, J.R. (2002). A guide to common freshwater invertebrates of North America. McDonald & Woodward. B Smith, D.G. (2001). Pennak's freshwater invertebrates of the United States: Porifera to Crustacea. C Kupferberg, S.J. (1997). The role of larval diet in anuran metamorphosis. Amer Zool, 37, 146-159 D Thorp, J.H. & Covich A.P., eds. (2010). Ecology and classification of North American freshwater invertebrates. E Gut content analyses performed by AM Hein or AK Fahimipour F Field observation of feeding event by AM Hein or AK Fahimipour G Arnett, R. (1971). The Beetles of United States, a manual for identification. Am Entomol Inst. H Blatchley, W.S. (1912). Coleoptera of Florida, complete set of papers published in Canadian Entomologist and Entomological News, 1912-1930. I Merritt, R.W. & Cummins K.W., eds. (1996). An introduction to the aquatic insects of North America. J Consultation with: Sharon Fitz-Coy Program in Fisheries & Aquatic Sciences University of Florida Gainesville, FL 32653 Mary Cichra Program in Fisheries & Aquatic Sciences University of Florida Gainesville, FL 32653 Gary Warren Florida Fish & Wildlife Conservation Commission University of Florida Gainesville, FL 32653 K Henry, T.J. and Froesher, R., eds. (1988). Catalog of Heteroptera or true bugs of Canada and continental United States. Table S1.2 Notes Consumer and resource IDs correspond to those in the taxon list found in Table S1.1. Approximately 56% of taxa were assigned feeding links via either gut content analyses or personal observations made in the field by AKF or AMH. Only feeding relationships between co-occurring taxa are shown here; potential links between taxa that never cooccurred in experimental mesocosms are omitted from this list. A complete consumerresource matrix containing the composite food web with all potential links between species is given as an electronic supplement associated with this article. No consumer diets in our dataset were determined solely by discussion with experts (source J in Table S1.2). All feeding links with expert sources were also supported by at least one literature source, or gut content analyses or personal observations in the field by AKF and AMH. These expert opinions were sought in order to add confidence to our dataset.