Supporting Information C: Invertebrate taxa comparisons
Table S6. Benthic invertebrate taxa found at ≥4 of the biodiversity sites and the number of sites
these taxa were detected in each land use category. Associated life-habit and primary functional
feeding groups are highlighted. Codes refer to the shortened names of the taxa highlighted in Fig.
4.
Order
Family:
Sub-family
Taxon
Plecoptera
Nemouridae
Nemoura trispinosa Claassen, 1923
Sprawler
Shredder
Nem
Number
of Sites
Present
(For,
Ag)
8 (4, 4)
Plecoptera
Nemouridae
Amphinemura wui/nigritta
Sprawler
Shredder
Amp
7 (4, 3)
Plecoptera
Leuctridae
Leuctra ferruginea (Walker, 1851)
Sprawler
Shredder
Leu
4 (1, 3)
Plecoptera
Chloroperlidae
Sweltsa naica (Provancher, 1876)
Clinger
Predator
Swe
8 (4, 4)
Trichoptera
Lepidostomatidae
Lepidostoma vernale/sommermanae
Climber
Shredder
Lep
8 (4, 4)
Trichoptera
Thremmatidae
Neophylax aniqua Ross, 1947
Clinger
Scraper
Nep
5 (3, 2)
Trichoptera
Limnephilidae
Hesperophylax designatus (Walker, 1852)
Sprawler
Shredder
Hesp
7 (3, 4)
Trichoptera
Limnephilidae
Onocosmoecus unicolor (Banks, 1897)
Sprawler
Shredder
Ono
4 (3, 1)
Coleoptera
Dytiscidae
Hydroporinae n.det
Swimmer
Predator
Hyd
5 (3, 2)
Diptera
Pediciidae
Dicranota
Burrower
Predator
Dic
4 (1, 3)
Diptera
Pediciidae
Chironomidae:
Podonominae
Chironomidae:
Chironominae
Chironomidae:
Diamesinae
Chironomidae:
Orthocladiinae
Chironomidae:
Orthocladiinae
Chironomidae:
Orthocladiinae
Chironomidae:
Orthocladiinae
Chironomidae:
Orthocladiinae
Chironomidae:
Orthocladiinae
Chironomidae:
Orthocladiinae
Chironomidae:
Orthocladiinae
Chironomidae:
Orthocladiinae
Chironomidae:
Orthocladiinae
Pedicia margarita Alexander, 1929
Burrower
Ped
5 (1, 4)
Parochlus kiefferi (Garrett, 1925)
Sprawler
Par
5 (3, 2)
Tanytarsini n.det (Primarily Micropsectra)
Climber
Tanyt
8 (4, 4)
Pseudodiamesa
Sprawler
Predator
CollectorGatherer
CollectorGatherer
CollectorGatherer
Pse
4 (3, 1)
Brillia parva Johannsen, 1934
Sprawler
Bri
5 (3, 2)
Corynoneura
Sprawler
Cor
7 (4, 3)
Eukiefferiella
Sprawler
Euk
6 (3, 3)
Heterotrissocladius marcidus (Walker, 1856)
Sprawler
Heterotr
6 (2, 4)
Hydrobaenus
Sprawler
Hydrob
7 (4, 3)
Metriocnemus
Burrower
Met
7 (3, 4)
Orthocladius/Cricotopus
Sprawler
Ort
7 (4, 3)
Paraphaenocladius/Parametriocnemus
Sprawler
Parap/Param
5 (2, 3)
Platysmittia/Psilometriocnemus
Sprawler
Pl/Ps
6 (3, 3)
Rheocricotopus
Sprawler
Rhe
6 (3, 3)
Diptera
Diptera
Diptera
Diptera
Diptera
Diptera
Diptera
Diptera
Diptera
Diptera
Diptera
Diptera
Diptera
Life-Habit
Functional
Feeding
Group
Code
Shredder
CollectorGatherer
CollectorGatherer
CollectorGatherer
Scraper
CollectorGatherer
CollectorGatherer
CollectorGatherer
CollectorGatherer
CollectorGatherer
Table S6.
(cont.)
Order
Family:
Sub-family
Taxon
Life-Habit
Functional
Feeding
Group
Code
Number
of Sites
Present
(For,
Ag)
Trombidiformes
Chironomidae:
Orthocladiinae
Hydryphantidae
Trombidiformes
Hydrovolziidae
Hydrovolzia
Climber
Predator
Hydrov
8 (4, 4)
Trombidiformes
Lebertiidae
Lebertia
Climber
Predator
Leb
7 (4, 3)
Trombidiformes
Sperchonidae
Sperchon
Climber
Predator
CollectorGatherer
CollectorGatherer
CollectorGatherer
CollectorGatherer
CollectorFilterer
CollectorGatherer
CollectorGatherer
Spe
8 (4, 4)
Ori
8 (4, 4)
Cyc
4 (1, 3)
Har
5 (3, 2)
Ost
4 (1, 3)
Pis
7 (3, 4)
Mega
6 (3, 3)
Micr
6 (3, 3)
Diptera
Thi
8 (4, 4)
Climber
CollectorGatherer
Predator
Pan
7 (4, 3)
Thienemanniella
Sprawler
Panisopsis
Oribatida
-
Oribatida
Burrower
Copepoda
-
Cyclopoida
Planktonic
Copepoda
-
Harpacticoida
Ostracoda
-
Ostracoda
Planktonic
Pisidium
Burrower
Bivalvia
Sphaeriidae
Climber
Oligochaeta
-
Megadriles
Burrower
Oligochaeta
-
Microdriles
Burrower
Table S7. Benthic invertebrate taxa found at at ≥4 Biodiversity sites and their contribution to
explaining the differences between the two land use types using Similarity of Percentages
analysis (SIMPER) in PRIMER-E. Taxa that made a contribution of less than 0.5% are indicated
by a dash (-), bolded values indicated the value is one of the ten highest contributors to similarity
or dissimilarity (Table 3).
Taxon
Nemoura
Amphinemura
Leuctra
Sweltsa
Lepidostoma
Neophylax
Hesperophylax
Onocosmoecus
Hydroporinae n.det
Dicranota
Pedicia
Parochlus
Tanytarsini n.det
Pseudodiamesa
Brillia
Corynoneura
Eukiefferiella
Heterotrissocladius
Hydrobaenus
Metriocnemus
Orthocladius/ Cricotopus
Paraphaenocladius/Parametriocnemus
Platysmittia/Psilometriocnemus
Rheocricotopus
Thienemanniella
Panisopsis
Hydrovolzia
Lebertia
Sperchon
Oribatida
Cyclopoida
Harpacticoida
Ostracoda
Pisidium
Megadriles
Microdriles
% Similarity
Forest sites
2.74
1.31
2.55
2.12
0.6
1.04
0.64
0.75
3.94
6.35
0.99
0.91
5.5
2.52
0.48
3.26
1.5
6.26
0.84
4.35
22.78
7.35
1.82
1.72
9.76
2.67
0.82
1.61
1.05
0.8
% Similarity
Agr sites
4.16
1.88
1.62
4.55
4.44
0.64
2.68
1.02
1.83
0.97
10.16
1.01
2.06
1.87
3.51
2.02
4.86
3.17
1.82
1.81
1.91
6.77
1.13
2.61
1.65
5.17
3.43
1.17
0.56
9.6
3.99
1.18
1.92
% Dissimilarity
1.14
2.15
0.93
1.08
2.42
0.71
0.64
0.64
0.79
0.55
4.71
5.97
0.71
1.11
3.2
3.64
1.42
4.11
1.93
4.97
2.25
0.87
2.82
10.27
4.24
1.93
0.67
3.96
0.85
4
1.26
8.05
2.34
0.97
-
Table S8. Median and mean (±) densities of invertebrate taxa found at ≥4 Biodiversity sites in
forested springs (n=4) and agricultural springs (n=4). Land-use comparisons were made with a
one-way ANOVA or a Mann-Whitney U test (indicated by ǂ). Statistically significant differences
(p≤0.05) are bolded
Taxon
Nemoura
Amphinemura
Leuctra
Sweltsa
Lepidostoma
Neophylax
Hesperophylax
Onocosmoecus
Hydroporonae n.det
Dicronata
Pedicia
Parochlus
Tanytarsini n.det
Pseudodiamesa
Brillia
Corynoneura
Eukiefferiella
Heterotrissocladius
Hydrobaenus
Metriocnemus
Orthocladius/
Cricotopus
Paraphaenocladius/
Parametriocnemus
Platysmittia/
Psilometriocnemus
Rheocricotopus
Thienemanniella
Panisopsis
Hydrovolzia
Lebertia
Sperchon
Oribatida
Cyclopoida
Harpacticoida
Ostracoda
Pisidium
Megadriles
Microdriles
Densities in Forested Sites
Median
Mean
±SE
Densities in Agricultural Sites
Median
Mean
±SE
180.95
25.60
0
179.76
163.69
27.98
66.67
23.59
32.74
0
0
2464.29
1178.95
46.37
61.90
1154.76
523.81
43.23
186.72
102.38
232.14
88.99
19.05
269.83
961.8
42.56
82.21
26.08
35.42
28.57
4.76
3172.62
2053.76
35.09
66.67
1739.29
1529.76
99.00
3236.22
86.90
106.18
65.65
19.05
143.25
848.59
25.14
45.29
12.26
16.60
28.57
4.76
1905.74
1228.55
11.71
29.86
1013.56
1202.81
73.08
3080.14
30.64
315.79
158.11
109.62
168.76
516.1
47.62
94.24
0
7.52
36.9
30.49
142.86
2928.57
0
119.05
238.10
142.86
285.71
357.14
476.19
423.4
646.94
107.19
367.01
487.63
61.90
83.63
4.76
47.93
59.81
28.94
285.71
8083.33
35.71
154.76
476.19
142.86
285.71
583.33
702.38
210.44
544.04
43.25
208.01
195.96
37.60
19.54
4.76
43.07
36.26
3.52
202.03
6154.88
35.71
93.99
331.63
61.48
137.46
377.15
396.21
0.499
0.388
0.130
0.693
0.879
0.951
0.640
0.152
0.858
0.376
0.010
0.241
0.427
0.556
0.757
0.261
ⱡ0.686
0.240
ⱡ0.686
0.130
2674.19
4469.24
2870.83
452.38
940.48
649.55
0.307
23.81
705.95
690.17
166.67
357.14
250.85
ⱡ0.686
47.62
39.29
13.52
142.86
119.05
45.59
1330.95
12919.05
1010.71
122.48
70.18
1913.78
152.38
0
76.19
0
124.81
47.62
38.78
1236.90
14864.29
1582.60
708.86
66.64
2854.51
299.53
638.39
99.70
3955.36
298.12
42.61
54.21
473.21
5748.55
762.17
622.35
20.79
1391.81
180.35
638.39
57.09
3955.36
216.96
16.09
30.72
119.05
523.81
85.21
245.11
112
226.07
141.19
64.16
76.19
3723.28
314.57
77.54
183.43
428.57
1357.14
1750.08
316.87
105.37
361.60
156.92
3292.89
253.56
6296.73
1069.98
186.39
179.43
351.54
947.91
1693.60
179.22
41.07
150.98
53.39
3250.17
205.92
3918.11
852.31
137.30
80.20
p-Value
0.333
0.294
0.002
ⱡ0.342
0.747
0.778
0.051
0.553
ⱡ0.342
0.757
0.467
0.408
0.436
0.322