Table S1 Mean biomass for individuals of thirty taxa from standing stock invertebrate resources that had more than 20 individuals and were randomly subsampled to obtain biomass of each taxon. The best fit model of the distribution of each taxon, the best fit model parameters that were used to calculate the theoretical mean mass of each taxon, the Kolmogorov–Smirnov goodness of fit p-value, and the calculated theoretical mean mass of each taxon are given below. Taxa Aquatic Heptageniidae Leptophlebiidae Ameletidae Baetidae Ephemerellidae Leuctridae Peltoperlidae Perlodidae Nemouridae Chloroperlidae Philopotamidae Chironomidae larvae Chironomidae pupa Simuliidae Tipulidae Gerridae Adult Aquatic Heptageniidae Ephemerellidae Leptophlebiidae Baetidae Nemouridae Leuctridae Chironomidae Terrestrial Araneae Coleoptera Diptera Homoptera Hymenoptera Lepidoptera Psocoptera 1General Mean Mass (mg) Extreme Value Model Model Parameters P-value 0.8042 0.2166 0.7828 0.1103 0.8800 0.1848 0.1996 0.4967 0.4025 0.1424 0.4221 0.1061 0.1269 0.1538 1.4326 13.0391 Exponential Lognormal GEV1 Gamma GEV1 Lognormal Lognormal Log-Logistic GEV1 GEV1 GEV1 Lognormal GEV1 GEV1 Lognormal Lognormal 1.2435 0.71444; -1.785 -0.32545; 0.31289; 0.68087 2.0042; 0.05503 0.10948; 0.37905; 0.61552 0.43549; -1.7834 0.65839; -1.8282 1.6574; 0.24836 -0.52797; 0.15743; 0.36894 -0.29653; 0.07885; 0.11529 0.50241; 0.15891; 0.17509 1.6544; -3.6118 0.68559; 0.03121; 0.0428 -0.35926; 0.06966; 0.13251 0.95499; -0.09649 1.7245; 1.081 0.613 0.958 0.992 0.941 0.882 0.962 0.992 0.990 0.860 0.971 0.697 0.990 0.791 0.945 0.842 0.247 0.4717 0.6962 0.1532 0.2328 0.6378 0.2747 0.1169 GEV1 Log-Logistic Log-Logistic Inverse Gaussian GEV1 GEV1 Gamma 0.32652; 0.14942; 0.31512 2.0051; 0.44431 4.6276; 0.14173 0.70428; 0.2328 -0.18384; 0.26749; 0.52519 0.09249; 0.10725; 0.2021 1.6012; 0.07298 0.173 0.327 0.915 0.973 0.955 0.519 0.532 5.0043 2.3522 0.2208 0.7952 0.7111 0.4479 0.3496 Lognormal Log-Logistic GEV1 Power Function GEV1 Exponential GEV1 1.899; -0.1928 1.1777; 0.4025 -0.25125; 0.13947; 0.16871 0.60325; 0; 2.1133 0.49501; 0.25941; 0.31528 2.2327 0.57438; 0.14001; 0.08586 0.705 0.783 0.986 0.485 0.701 0.974 0.623 Table S2 Invertebrate biomass in Dry Run (DR) and Union Springs (US) when pools have flow coming into them (F) and when pools are completely isolated (I) during summer 2011. Mean Biomass ± SE (mg/m2/day) Flow Stream Status DR F F F I I US F F F I I Date Drift (D) 13-Jun 10.29 ± 2.53 30-Jun 58.05 ± 21.60 27-Jul 6.76 ± 4.28 10-Aug 0.53 ± 0.53 25-Aug 2.33 ± 0.53 15-Jun 9.42 ± 3.10 1-Jul 24.69 ± 6.75 26-Jul 6.90 ± 2.24 11-Aug 0.66 ± 0.30 24-Aug 0.55 ± 0.37 Benthic (B) 202.55 ± 10.75 184.64 ± 40.70 157.24 ± 84.58 37.00 ± 10.60 57.60 ± 10.60 280.70 ± 98.65 227.77 ± 70.85 146.84 ± 48.46 45.71 ± 19.98 59.58 ± 28.62 Terrestrial (T) 6.76 ± 2.71 4.21 ± 1.75 6.95 ± 3.25 6.92 ± 1.03 14.34 ± 1.03 0.79 ± 3.75 3.20 ± 2.87 158.88 ± 152.78 2.01 ± 3.41 20.39 ± 16.05 Adult Aquatic (A) 5.26 ± 1.26 2.6 ± 0.69 0.61 ± 0.30 1.44 ± 0.43 0.83 ± 0.43 2.80 ± 1.83 2.57 ± 0.93 0.12 ± 0.08 1.90 ± 1.64 0.02 ± 0.02 Emerging Adult Aquatic 0.84 ± 0.53 6.50 ± 1.64 0.82 ± 0.33 2.04 ± 0.54 2.22 ± 0.59 1.88 ± 0.46 3.20 ± 1.28 2.40 ± 0.99 1.15 ± 0.48 1.57 ± 0.57 Table S3 The top 10 taxa in brook trout diet for July 25 – August 25 at Dry Run (A) and Union Springs (B) ranked by percent composition by abundance. T, AA, and A indicate terrestrial, adult aquatic, and aquatic taxa respectively. A Dry Run Exclosure No Exclosure % of % of Order Family Type diet Order Family Type diet Diptera Empididae T 44.3 Homoptera NA T 15.7 Diptera NA AA 9.0 Hymenoptera NA T 14.2 Diptera NA T 8.4 Diptera NA T 11.5 Diptera Chiron. pupa A 7.8 Plecoptera NA AA 8.0 Diptera Chiron. larvae A 4.2 Diptera Empididae T 7.7 Ephemeroptera NA AA 3.6 Ephemeroptera NA A 5.4 Nematoda NA A 3.0 Coleoptera NA T 4.6 Coleoptera NA T 3.0 Plecoptera NA A 3.4 Homoptera NA T 3.0 Psocoptera NA T 3.1 Hymenoptera NA T 2.4 Diptera Chiron. pupa A 2.7 B Union Springs Exclosure Order Family Type Diptera Chiron. larvae A Plecoptera NA A Trichoptera NA A Hymenoptera NA T Ephemeroptera NA A Diptera Other A Hemiptera NA A Diptera NA AA Coleoptera NA T Homoptera NA T No Exclosure % of diet 25.0 12.5 9.4 9.4 6.3 6.3 6.3 6.3 6.3 6.3 Order Hymenoptera Coleoptera Homoptera Ephemeroptera Trichoptera Diptera Diptera Diptera Nematoda Plecoptera Family NA NA NA NA NA Empididae NA NA NA NA Type T T T A A T AA T A A % of diet 15.4 12.3 12.3 10.8 9.2 7.7 6.2 6.2 4.6 3.1 Table S4 The top 10 taxa in brook trout diet for July 25 – August 25 at Dry Run (A) and Union Springs (B) ranked by percent composition by mass. T, AA, and A indicate terrestrial, adult aquatic, and aquatic taxa respectively. A Dry Run Exclosure No Exclosure % of % of Order Family Type diet Order Family Type diet Diptera Empididae T 51.3 Homoptera NA T 17.5 Hemiptera NA A 11.4 Coleoptera NA T 15.2 Coleoptera NA T 10.3 Hymenoptera NA T 14.1 Homoptera NA T 3.5 Diptera Empididae T 8.5 Diptera NA T 2.7 Araneae NA T 5.4 Hymenoptera NA T 2.5 Plecoptera NA AA 5.2 Ephemeroptera NA AA 2.0 Ephemeroptera NA A 4.4 Diptera Chiron. pupa A 1.4 Diptera NA T 3.6 Diptera NA AA 1.3 Psocoptera NA T 1.5 Trichoptera NA AA 1.0 Hemiptera NA T 1.4 B Union Springs Exclosure Order Coleoptera Hymenoptera Homoptera Diptera Ephemeroptera Trichoptera Diptera Plecoptera Hemiptera Plecoptera Family NA NA NA Other NA NA Chiron. larvae NA NA NA No Exclosure Type T T T A A A A A A AA % of diet 26.0 11.8 8.8 8.8 8.7 7.0 4.7 4.2 2.2 1.5 Order Family Type Coleoptera NA T Hymenoptera NA T Homoptera NA T Ephemeroptera NA A Diptera Empididae T Trichoptera NA A Diptera NA T Plecoptera NA AA Diptera NA AA Ephemeroptera NA AA % of diet 38.1 14.4 12.9 11.1 8.0 5.1 1.8 1.1 0.9 0.8