MCB 730: MOLECULAR AND CELLULAR BIOLOGY LABORATORY

advertisement
PBIO 3300/5300: PLANT GENETICS
(Class Numbers 5196 & 5199 respectively – 3.0 Credit Hours)-Spring 2013
Time & Place: Monday, Wednesday and Friday 8:35 – 9:30 AM; Porter 104
Instructor: Dr. Allan Showalter
Course web site: http://www.ohio.edu/plantbio/staff/showalte/PBIO 3300 & 5300/
Text: Introduction to Genetic Analysis (10th edition) ©2012 by Anthony J. F. Griffiths et al.
Prerequisites: BIOS 1700 or PBIO 1140
Course Description:
The purpose of this course is to provide students with an understanding of the fundamentals of plant
genetics. Topics include Mendelian and Non-Mendelian genetics, gene structure and function, genomics,
plant breeding systems, population genetics, and evolution.
Learning Outcomes:
1. Understand the basic principles of Mendelian and non- Mendelian genetics and be able to use those
principles to solve problems.
2. Understand and be able to explain the fundamentals of transcription and translation and the enzyme
complexes involved.
3. Learn how genetic and molecular genetic research is done and be familiar with the techniques
involved and what they do.
4. Understand how plant genetics differs from animal genetics.
Grades will be based on the following:
1. A midterm exam on the first third of the course (100 points).
2. A midterm exam on the second third of the course (100 points).
3. A comprehensive final exam (100 points).
4. Homework (100 points). See assigned homework problems and instructions for completing them on
page 3 of the syllabus.
5. Graduate students enrolled in the course will additionally have to submit an 8-10 page double spaced
paper (50 points) on a genetics topic approved by the instructor. The paper must follow the format of
a review paper in Nature Reviews Molecular Cell Biology (http://www.nature.com/nrm/index.html),
being sure to include complete article titles for all references.
Thus, there are 400 points possible for PBIO 3300 students and 450 points possible for PBIO 5300
students. Typically, 93.3% and above will earn an A, 90-93.3% an A-, 86.7-90% a B+, 83.3-86.7% a B,
80-83.3% a B-, 76.7-80% a C+, 73.3-76.7% a C, 70-73.3% a C-, 66.7-70% a D+, 63.3-66.7% a D, 6063.3% a D-, and below 60% an F.
Exams will be based upon material covered in class lectures as well as in the assigned readings and
homework problems.
Office Hours:
Allan Showalter- By appointment, Porter 504, phone 593-1135.
Email: showalte@ohio.edu
Academic Conduct:
The penalty for course-related academic dishonesty (i.e., cheating on exams, plagiarism, etc.) will result
in a grade of zero for that assignment and may be reported to Judiciaries for further action. Cell phones
must be turned off during class; violators will be asked to bring treats for the class.
Attendance Policy:
1
Attendance is highly recommended. Any absences must be well justified and explained to the instructor
in advance in order to make up any of the graded material.
2
PBIO 3300/5300: PLANT GENETICS
Syllabus - Spring 2013
WEEK
1
2
3
4
5
6
7
8
9
10
Review
11
12
13
14
15
DATE
Jan. 14
Jan. 16
Jan. 18
Jan. 21
Jan. 23
Jan. 25
Jan. 28
Jan. 30
Feb. 1
Feb. 4
Feb. 6
Feb. 8
Feb. 11
Feb. 13
Feb. 15
Feb. 18
Feb. 20
Feb. 22
Feb. 25
Feb. 27
March 1
March 4
March 6
March 8
March 11
March 13
March 15
March 18
March 20
March 22
CHAPTER
1
1; pp. 759-775
2
No Class
2
3
3
4
4
6
6
7
7
8
8
9
9
10
10
No Class
No Class
No Class
12
12
13
14
14
pp. 776-777
TOPICS
Introduction; The genetics revolution
Genetic model organisms; genetic nomenclature
Single–gene inheritance
Martin Luther King, Jr. Day holiday
Single–gene inheritance
Independent assortment of genes
Independent assortment of genes
Mapping eukaryotic chromosomes by recombination
Mapping eukaryotic chromosomes by recombination
Gene interaction
Gene interaction
Review
Exam I
DNA structure and replication
DNA structure and replication
RNA: transcription and processing
RNA: transcription and processing
Proteins and their synthesis
Proteins and their synthesis
Gene isolation and manipulation
Gene isolation and manipulation
Spring Break
Spring Break
Spring Break
Regulation of gene expression in eukaryotes
Regulation of gene expression in eukaryotes
The genetic control of development
Genomes and genomics
Genomes and genomics
Bioinformatic resources for genetics & genomics;
March 25
March 27
March 29
April 1
April 3
April 5
April 8
April 10
April 12
April 15
April 17
April 19
April 22
April 24
April 26
15
16
16
17
17
18
18
19
19
20
20
-
Exam II
The dynamic genome: transposable elements
Mutation, repair, and recombination
Mutation, repair, and recombination
Large scale genomic changes
Large scale genomic changes
Population genetics
Population genetics
The inheritance of complex traits
The inheritance of complex traits
Evolution of genes and traits
Evolution of genes and traits
Plant breeding systems
Macroevolutionary processes
Review
May 1 (Wed.)
Final Exam (8:00 A.M.)
3
PBIO 3300/5300: PLANT GENETICS
Homework Problems from Introduction to Genetic Analysis (10th edition)
Complete and submit the assigned homework problems listed for each chapter on Mondays in
class (or at the very next class meeting in the case of any holidays). Submit your answers as a
printed word document; additional graphical information can be added by hand as needed. Once
chapters are covered or introduced in lecture, homework assignments for those chapters will be
due on the next Monday. Due dates may be adjusted by the instructor if necessary. Each chapter
homework assignment is worth 5 points and two additional homework assignments (see bottom
of this page) are worth 5 points each, resulting in 100 possible points for homework.
Chapter
1
Homework Problems
1, 2, 3, 6, 8, 11, 12, 14, 15, 17
2
17, 18, 20, 27, 28, 34, 36, 41, 44, 47
3
12, 13,18, 20, 24, 25, 29, 31, 36, 37
4
12, 13, 14, 17, 18, 24, 28, 33, 49, 61
6
1, 5, 13, 16, 20, 23, 24, 25, 35, 41
7
3, 4, 6, 8, 9, 10, 16, 26, 29, 32
8
1, 3, 8, 12 ,14, 18, 21, 23, 26, 28
9
4, 10, 11, 15, 17, 23, 28, 30, 37, 39
10
2, 3, 6, 7, 10, 18, 20, 23, 24, 27
12
2, 8, 12, 13, 15, 19, 23, 24, 26, 34
13
1, 2, 3, 4, 5, 6, 7, 8, 9, 10
14
5, 9, 10, 12, 21, 24, 25, 27, 28, 32
15
1, 3, 8, 11, 12, 13, 15, 16, 18, 19
16
2, 4, 12, 14, 15, 20, 21, 23, 26, 27
17
1, 5, 9, 13, 17, 21, 24, 28, 35, 43
18
1, 2, 3, 4, 5, 6, 7, 8, 9, 10
19
1, 2, 3, 4, 5, 6, 7, 8, 9, 10
20
1, 2, 3, 4, 5, 6, 7, 8, 9, 10
Two additional homework assignments to be completed and submitted any time during the
semester. These assignments should be printed as word documents and submitted in class.
1. Who is your favorite geneticist? Justify your answer in 1 page. (5 points).
2. Write a 1 page summary of any genetics-based seminar you attended this semester. (5 points).
4
Download