Bio248 Syllabus
2012
Bio248S - From Genes to Genomes
Instructor:
Office:
Tel:
Email:
Text:
Jinnie M. Garrett
SC 2083
x 4716
jgarrett@hamilton.edu
Lectures: MWF 10:00 -10:50am, SCCT 2048
Lab:
W 1:00 -3:50pm,
SCCT 2091
Office hrs: Sun 7:00-8.00pm, R 10:30-11:30am.
Help sessions: Some Sundays 7pm SCCT 2048
Genetics: From Genes to Genomes. 4th Ed. Hartwell et al, McGraw-Hill 2011
Lecture Outline
Week
Date
Day
Lecture Topic
Chapter(s)
1
1/16
1/18
1/20
M
W
F
Course introduction MLK Day
Human genetics: history - eugenics
Mechanisms of inheritance
1,2,3
**
2
2
1/23
1/25
1/26
M
W
F
Basic principles: linkage, recombination, mapping
Basic principles: 3-point crosses
Genetic mapping, recent examples
4, 5
5
5
3
1/30
2/1
2/3
M
W
F
DNA & chromosome structure
DNA & chromosome replication
Recombination – homologous & specialized
6, 12
6, 12
6
4
2/6*
2/8
2/10
M
W
F
Mutations – chromosome level.
Mutation – classification and causes
DNA repair
14
7
7
5
2/13
2/15
2/17
M
W
F
Gene complementation analysis
TBA
Newborn genetic screening PKU
7
**
6
2/20* M
2/22 W
2/24 F
Problems and help session for Exam 1
Gene expression: mRNA synthesis
Gene expression: protein synthesis
**
8
8
7
2/27
2/29
3/2
Recombinant DNA
Molecular determination of genotype
GM plants & animals
9
9
9
M
W
F
1
Bio248 Syllabus
2012
Week
Date
Day Lecture Topic
Chapter(s)
8
3/5
3/7
3/9
M
W
F
10
10
**
9
3/26
3/28
3/30
M
W
F
Genomics – genetic & physical maps
Global analysis of gene products
DNA & the Law
SPRING BREAK
DNA variation & its detection
Genomics – positional cloning
Cont, problems
10
4/2*
4/4
4/6
M
W
F
Haplotypes: molecular population studies
TBA
Genetic analysis in prokaryotes
21
11
4/9
4/11
4/13
M
W
F
Genetic regulation in prokaryotes
Cont.
Gene regulation in eukaryotes: transcription
17
17
18
12
4/16
4/18
M
W
18
18
4/20
F
Gene regulation in eukaryotes: post- transcription
Epigenetics; ‘Ghost in our Genes’
7.30pm Required seminar – Dr. Dorothy Roberts
DTC testing
**
13
4/23
4/25
4/27
M
W
F
Cell cycle & cancer genetics
Genetic counseling
Genetics of development
19
**
20
14
4/30
5/2
5/4
5/7
M
W
F
M
Genetics of development
Gene therapy & cloning
GMHs: Our posthuman future
FINAL EXAM 7pm
20
**
**
NOTE
11
11
11
15
Required seminar 7:30pm KJ Aud.
4/18/2012 Dr Dorothy Roberts Northwestern University “TBA”
Choice Seminars 4:10pm SCCT3024
2/6/12 Dr Engda Hagos, Colgate University. "Genetic Instability in Mouse Embryonic
Fibroblasts"
2/20/12 Valerie Valant ’09, Mass. General Hospital. “An Introduction to Medical
Genetics: Using a comparative approach to differentiate Alzheimer's Disease from
Cerebral Amyloid Angiopathy”
4/2/12 Dr Eddy Rubin, Joint Genome Institute. “"Dark matter of the human genome
and bit of the Neanderthal genome"”
2
Bio248 Syllabus
2012
Laboratory Outline
Week
Date
1
2
3
4
5
6
7
8
9
10
11
12
13
14
1/18
1/25
2/1
2/8
2/15
2/22
2/29
3/7
3/28
4/4
4/11
4/18
4/25
5/2
Laboratory
Assignment
Intro to lab/ safety/ methods
Techniques in bacterial genetics
Mutagenesis – Ames test 1 – known mutagens
Mutagenesis – Ames test 2 – test unknowns
DNA- The Genetic Material
EXAM I
PCR/clone libraries
Plasmid preps/ digests – Pandora’s Box
Bioinformatics
EXAM II
Gene Expression – The lac operon
Gene Expression – nitrogen catabolite repression
Lab test
Model mutant presentations
Worksheet 1
Worksheet 2
Worksheet 3
Worksheet 4
Worksheet 5
Worksheet 6
Summary
Evaluation
3 exams @ 100pts each
300
8 Problem sets (5pts) & class participation
60
Seminar summaries (2 @10pts)
20
1 required (Dorothy Roberts, 4/18) + 1 choice
Model mutant presentation & paper
20
Lab worksheets (unequal value)
75
Lab test
25
Total pts
500
Exams: will be primarily problem-based and require interpretation and analysis of data
rather than memorization of a particular set of facts. In class exams will be taken during the
lab period and will be open book, you may bring your textbook and class notes.
Problem sets: covering the week’s subject matter will be assigned on Monday and should be
handed in during the following Monday’s class for full credit.
Seminar summaries: You are required to attend the seminar on 4/18 and 1 other of the three
seminars listed on the previous page. In both cases you should write a 1 page summary of the
information presented, concluding with one question that you could have asked the speaker.
Model mutant presentations: Working in groups of four, you will be required to select a
recent paper discussing a topic covered in the first half of the semester in which one of the
‘model organisms’ (yeast, Arabadopsis, C.elegans, Drosophila, mouse) is used as the
3
Bio248 Syllabus
2012
experimental organism. You should prepare an overview of the research to present to the
class during lab as an 8-10min oral presentation and also hand in a 2 page summary of your
paper.
Lab Worksheets: You will be given a copy of the worksheet in lab to record observations
and measurements. There will be an electronic copy on BlackBoard and you should transfer
your data and fill in the interpretation of the results on a clean worksheet, print it & hand it
in. Do NOT hand in the scruffy hand written notes from lab – I will not give them a grade.
Lab work is often done in groups and your data should be identical to your partner, however,
the data interpretation and all calculations should be done independently so your lab
worksheets should not be identical!
Course participation: You are allowed 2 unexplained class absences. You will be deducted
10pts per class missed over the 2 permitted. Students who are members of winter athletic
teams should notify me in writing of required class or lab absences for athletic events by the
end of week 1 (1/20/12). Spring athletic team members should notify me in writing of
required class or lab absences for athletic events by the end of week 6 (2/24/2012).
Students with disabilities: Hamilton College will make reasonable accommodations for
students with properly documented disabilities. If you are eligible to receive an
accommodation(s) and would like to make a formal request for this course, please discuss it
with me during the first two weeks of class. You will need to provide Allen Harrison,
Associate Dean of Students (Elihu Root House; ext. 4021) with appropriate documentation of
your disability.
4