mcb 730: molecular and cellular biology laboratory

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PBIO 3300/5300: PLANT GENETICS
(Class Numbers 2906 & 2907 respectively – 3.0 Credit Hours)-Spring 2014
Time & Place: Monday, Wednesday and Friday 8:35 – 9:30 AM; Porter 104
Instructor: Dr. Allan Showalter
Course web site: http://www.ohio.edu/plantbio/staff/showalte/PBIO 3300 & 5300/
Text: Introduction to Genetic Analysis (10th edition) ©2012 by Anthony J. F. Griffiths et al.
Prerequisites: BIOS 1700 or PBIO 1140
Course Description:
The purpose of this course is to provide students with an understanding of the fundamentals of plant
genetics. Topics include Mendelian and non-Mendelian genetics, gene structure and function, genomics,
plant breeding systems, population genetics, and evolution.
Learning Outcomes:
1. Understand the basic principles of Mendelian and non-Mendelian genetics and be able to use those
principles to solve problems.
2. Understand and be able to explain the fundamentals of transcription and translation and the enzyme
complexes involved.
3. Learn how genetic and molecular genetic research is done and be familiar with the techniques
involved and what they do.
4. Understand how plant genetics differs from animal genetics.
Grades will be based on the following:
1. A midterm exam on the first third of the course (100 points).
2. A midterm exam on the second third of the course (100 points).
3. A comprehensive final exam (100 points).
4. Homework (100 points). See assigned homework problems and instructions for completing them on
page 3 of the syllabus.
5. Graduate students enrolled in the course will additionally have to submit an 8-10 page double spaced
paper (50 points) on a genetics topic approved by the instructor. The paper must follow the format of a
review paper in Nature Reviews Molecular Cell Biology (http://www.nature.com/nrm/index.html),
being sure to include complete article titles for all references.
Thus, there are 400 points possible for PBIO 3300 students and 450 points possible for PBIO 5300
students. Typically, 93.3% and above will earn an A, 90-93.3% an A-, 86.7-90% a B+, 83.3-86.7% a B,
80-83.3% a B-, 76.7-80% a C+, 73.3-76.7% a C, 70-73.3% a C-, 66.7-70% a D+, 63.3-66.7% a D, 6063.3% a D-, and below 60% an F.
Exams will be based upon material covered in class lectures as well as in the assigned readings and
homework problems.
Office Hours:
By appointment, Porter Hall-Room 504 (phone: 740-593-1135 or email: showalte@ohio.edu)
Academic Conduct/Misconduct:
The penalty for course-related academic dishonesty (i.e., cheating on exams, plagiarism, etc.)
will be failure of the entire course along with a report of the incident being sent to University
Judiciaries. Note that University Judiciaries may impose additional sanctions, and students may
appeal academic sanctions through the grade appeal process. Cell phones must be turned off
during class; violators will be asked to bring treats for the class.
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Attendance Policy:
Attendance is highly recommended. Any absences must be well justified and explained to the
instructor in advance in order to make up any of the graded material.
Intellectual Property:
The lectures, classroom activities, and all materials associated with this class and developed by
the instructor are copyrighted in the name of Allan Showalter on August 26, 2013.
Accommodations for Students with a Disability Policy:
Any student who suspects s/he may need an accommodation based on the impact of a disability
should contact the class instructor privately to discuss the student’s specific needs and provide
written documentation from the Office of Student Accessibility Services. If the student is not yet
registered as a student with a disability, s/he should contact the Office of Student Accessibility
Services.
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PBIO 3300/5300: PLANT GENETICS
Syllabus - Spring 2014
WEEK
1
2
3
4
5
6
7
8
9
10
Review
11
12
13
14
15
DATE
Jan. 13
Jan. 15
Jan. 17
Jan. 20
Jan. 22
Jan. 24
Jan. 27
Jan. 29
Jan. 31
Feb. 3
Feb. 5
Feb. 7
Feb. 10
Feb. 12
Feb. 14
Feb. 17
Feb. 19
Feb. 21
Feb. 24
Feb. 26
Feb. 28
March 3
March 5
March 7
March 10
March 12
March 14
March 17
March 19
March 21
CHAPTER
1
1; pp. 759-775
2
No Class
2
3
3
4
4
6
6
7
7
8
8
9
9
10
10
No Class
No Class
No Class
12
12
13
14
14
pp. 776-777
TOPICS
Introduction; The genetics revolution
Genetic model organisms; genetic nomenclature
Single–gene inheritance
Martin Luther King, Jr. Day holiday
Single–gene inheritance
Independent assortment of genes
Independent assortment of genes
Mapping eukaryotic chromosomes by recombination
Mapping eukaryotic chromosomes by recombination
Gene interaction
Gene interaction
Review
Exam I
DNA structure and replication
DNA structure and replication
RNA: transcription and processing
RNA: transcription and processing
Proteins and their synthesis
Proteins and their synthesis
Gene isolation and manipulation
Gene isolation and manipulation
Spring Break
Spring Break
Spring Break
Regulation of gene expression in eukaryotes
Regulation of gene expression in eukaryotes
The genetic control of development
Genomes and genomics
Genomes and genomics
Bioinformatic resources for genetics & genomics;
March 24
March 26
March 28
March 31
April 2
April 4
April 7
April 9
April 11
April 14
April 16
April 18
April 21
April 23
April 25
15
16
16
17
17
18
18
19
19
20
20
-
Exam II
The dynamic genome: transposable elements
Mutation, repair, and recombination
Mutation, repair, and recombination
Large scale genomic changes
Large scale genomic changes
Population genetics
Population genetics
The inheritance of complex traits
The inheritance of complex traits
Evolution of genes and traits
Evolution of genes and traits
Plant breeding systems
Macroevolutionary processes
Review
April 30 (Wed.)
Final Exam (8:00 A.M.)
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PBIO 3300/5300: PLANT GENETICS
Homework Problems from Introduction to Genetic Analysis (10th edition)
Complete and submit the assigned homework problems listed for each chapter on Mondays in
class (or at the very next class meeting in the case of any holidays). Submit your answers as a
printed word document; additional graphical information can be added by hand as needed. Once
chapters are covered or introduced in lecture, homework assignments for those chapters will be
due on the next Monday. Due dates may be adjusted by the instructor if necessary. Each chapter
homework assignment is worth 5 points and two additional homework assignments (see bottom
of this page) are worth 5 points each, resulting in 100 possible points for homework.
Chapter
1
Homework Problems
1, 2, 3, 6, 8, 11, 12, 14, 15, 17
2
17, 18, 20, 27, 28, 34, 36, 41, 44, 47
3
12, 13,18, 20, 24, 25, 29, 31, 36, 37
4
12, 13, 14, 17, 18, 24, 28, 33, 49, 61
6
1, 5, 13, 16, 20, 23, 24, 25, 35, 41
7
3, 4, 6, 8, 9, 10, 16, 26, 29, 32
8
1, 3, 8, 12 ,14, 18, 21, 23, 26, 28
9
4, 10, 11, 15, 17, 23, 28, 30, 37, 39
10
2, 3, 6, 7, 10, 18, 20, 23, 24, 27
12
2, 8, 12, 13, 15, 19, 23, 24, 26, 34
13
1, 2, 3, 4, 5, 6, 7, 8, 9, 10
14
5, 9, 10, 12, 21, 24, 25, 27, 28, 32
15
1, 3, 8, 11, 12, 13, 15, 16, 18, 19
16
2, 4, 12, 14, 15, 20, 21, 23, 26, 27
17
1, 5, 9, 13, 17, 21, 24, 28, 35, 43
18
1, 2, 3, 4, 5, 6, 7, 8, 9, 10
19
1, 2, 3, 4, 5, 6, 7, 8, 9, 10
20
1, 2, 3, 4, 5, 6, 7, 8, 9, 10
Two additional homework assignments to be completed and submitted any time during the
semester. These assignments should be printed as word documents and submitted in class.
1. Who is your favorite geneticist? Justify your answer in 1 page. (5 points).
2. Write a 1 page summary of any genetics-based seminar you attended this semester. (5 points).
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