PBIO 3300/5300: PLANT GENETICS (Class Numbers 2906 & 2907 respectively – 3.0 Credit Hours)-Spring 2014 Time & Place: Monday, Wednesday and Friday 8:35 – 9:30 AM; Porter 104 Instructor: Dr. Allan Showalter Course web site: http://www.ohio.edu/plantbio/staff/showalte/PBIO 3300 & 5300/ Text: Introduction to Genetic Analysis (10th edition) ©2012 by Anthony J. F. Griffiths et al. Prerequisites: BIOS 1700 or PBIO 1140 Course Description: The purpose of this course is to provide students with an understanding of the fundamentals of plant genetics. Topics include Mendelian and non-Mendelian genetics, gene structure and function, genomics, plant breeding systems, population genetics, and evolution. Learning Outcomes: 1. Understand the basic principles of Mendelian and non-Mendelian genetics and be able to use those principles to solve problems. 2. Understand and be able to explain the fundamentals of transcription and translation and the enzyme complexes involved. 3. Learn how genetic and molecular genetic research is done and be familiar with the techniques involved and what they do. 4. Understand how plant genetics differs from animal genetics. Grades will be based on the following: 1. A midterm exam on the first third of the course (100 points). 2. A midterm exam on the second third of the course (100 points). 3. A comprehensive final exam (100 points). 4. Homework (100 points). See assigned homework problems and instructions for completing them on page 3 of the syllabus. 5. Graduate students enrolled in the course will additionally have to submit an 8-10 page double spaced paper (50 points) on a genetics topic approved by the instructor. The paper must follow the format of a review paper in Nature Reviews Molecular Cell Biology (http://www.nature.com/nrm/index.html), being sure to include complete article titles for all references. Thus, there are 400 points possible for PBIO 3300 students and 450 points possible for PBIO 5300 students. Typically, 93.3% and above will earn an A, 90-93.3% an A-, 86.7-90% a B+, 83.3-86.7% a B, 80-83.3% a B-, 76.7-80% a C+, 73.3-76.7% a C, 70-73.3% a C-, 66.7-70% a D+, 63.3-66.7% a D, 6063.3% a D-, and below 60% an F. Exams will be based upon material covered in class lectures as well as in the assigned readings and homework problems. Office Hours: By appointment, Porter Hall-Room 504 (phone: 740-593-1135 or email: showalte@ohio.edu) Academic Conduct/Misconduct: The penalty for course-related academic dishonesty (i.e., cheating on exams, plagiarism, etc.) will be failure of the entire course along with a report of the incident being sent to University Judiciaries. Note that University Judiciaries may impose additional sanctions, and students may appeal academic sanctions through the grade appeal process. Cell phones must be turned off during class; violators will be asked to bring treats for the class. 1 Attendance Policy: Attendance is highly recommended. Any absences must be well justified and explained to the instructor in advance in order to make up any of the graded material. Intellectual Property: The lectures, classroom activities, and all materials associated with this class and developed by the instructor are copyrighted in the name of Allan Showalter on August 26, 2013. Accommodations for Students with a Disability Policy: Any student who suspects s/he may need an accommodation based on the impact of a disability should contact the class instructor privately to discuss the student’s specific needs and provide written documentation from the Office of Student Accessibility Services. If the student is not yet registered as a student with a disability, s/he should contact the Office of Student Accessibility Services. 2 PBIO 3300/5300: PLANT GENETICS Syllabus - Spring 2014 WEEK 1 2 3 4 5 6 7 8 9 10 Review 11 12 13 14 15 DATE Jan. 13 Jan. 15 Jan. 17 Jan. 20 Jan. 22 Jan. 24 Jan. 27 Jan. 29 Jan. 31 Feb. 3 Feb. 5 Feb. 7 Feb. 10 Feb. 12 Feb. 14 Feb. 17 Feb. 19 Feb. 21 Feb. 24 Feb. 26 Feb. 28 March 3 March 5 March 7 March 10 March 12 March 14 March 17 March 19 March 21 CHAPTER 1 1; pp. 759-775 2 No Class 2 3 3 4 4 6 6 7 7 8 8 9 9 10 10 No Class No Class No Class 12 12 13 14 14 pp. 776-777 TOPICS Introduction; The genetics revolution Genetic model organisms; genetic nomenclature Single–gene inheritance Martin Luther King, Jr. Day holiday Single–gene inheritance Independent assortment of genes Independent assortment of genes Mapping eukaryotic chromosomes by recombination Mapping eukaryotic chromosomes by recombination Gene interaction Gene interaction Review Exam I DNA structure and replication DNA structure and replication RNA: transcription and processing RNA: transcription and processing Proteins and their synthesis Proteins and their synthesis Gene isolation and manipulation Gene isolation and manipulation Spring Break Spring Break Spring Break Regulation of gene expression in eukaryotes Regulation of gene expression in eukaryotes The genetic control of development Genomes and genomics Genomes and genomics Bioinformatic resources for genetics & genomics; March 24 March 26 March 28 March 31 April 2 April 4 April 7 April 9 April 11 April 14 April 16 April 18 April 21 April 23 April 25 15 16 16 17 17 18 18 19 19 20 20 - Exam II The dynamic genome: transposable elements Mutation, repair, and recombination Mutation, repair, and recombination Large scale genomic changes Large scale genomic changes Population genetics Population genetics The inheritance of complex traits The inheritance of complex traits Evolution of genes and traits Evolution of genes and traits Plant breeding systems Macroevolutionary processes Review April 30 (Wed.) Final Exam (8:00 A.M.) 3 PBIO 3300/5300: PLANT GENETICS Homework Problems from Introduction to Genetic Analysis (10th edition) Complete and submit the assigned homework problems listed for each chapter on Mondays in class (or at the very next class meeting in the case of any holidays). Submit your answers as a printed word document; additional graphical information can be added by hand as needed. Once chapters are covered or introduced in lecture, homework assignments for those chapters will be due on the next Monday. Due dates may be adjusted by the instructor if necessary. Each chapter homework assignment is worth 5 points and two additional homework assignments (see bottom of this page) are worth 5 points each, resulting in 100 possible points for homework. Chapter 1 Homework Problems 1, 2, 3, 6, 8, 11, 12, 14, 15, 17 2 17, 18, 20, 27, 28, 34, 36, 41, 44, 47 3 12, 13,18, 20, 24, 25, 29, 31, 36, 37 4 12, 13, 14, 17, 18, 24, 28, 33, 49, 61 6 1, 5, 13, 16, 20, 23, 24, 25, 35, 41 7 3, 4, 6, 8, 9, 10, 16, 26, 29, 32 8 1, 3, 8, 12 ,14, 18, 21, 23, 26, 28 9 4, 10, 11, 15, 17, 23, 28, 30, 37, 39 10 2, 3, 6, 7, 10, 18, 20, 23, 24, 27 12 2, 8, 12, 13, 15, 19, 23, 24, 26, 34 13 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 14 5, 9, 10, 12, 21, 24, 25, 27, 28, 32 15 1, 3, 8, 11, 12, 13, 15, 16, 18, 19 16 2, 4, 12, 14, 15, 20, 21, 23, 26, 27 17 1, 5, 9, 13, 17, 21, 24, 28, 35, 43 18 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 19 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 20 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 Two additional homework assignments to be completed and submitted any time during the semester. These assignments should be printed as word documents and submitted in class. 1. Who is your favorite geneticist? Justify your answer in 1 page. (5 points). 2. Write a 1 page summary of any genetics-based seminar you attended this semester. (5 points). 4