The University of Jordan
Faculty: Medicine
Department: Pathology, Microbiology and Forensic Medicine.
nd
Semester: 2
Academic Year: 2013/2014
Analytical Toxicology (0501703)
Credit hours
3.0
Coordinator
/ Lecturer
Prof. Kamal A. Hadidi
Course
website
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Office hours
Day
Time
Sunday
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1
Prerequisite
----------
Office
number
210
Office phone
23494
E-mail
khadidi@ju.edu.jo
Level
Monday
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Tuesday
----------------
Place
Wednesday
9:00 – 12:00
2nd floor
Thursday
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Course Description
Analytical Toxicology course covers the theoretical and practical aspects of methods of poison
separation, purification, and quantification. Also covers the techniques applied for the above purpose
such as different methods of chromatography (like; TLC, HPLC, GC…) and spectroscopy (like;
spectrophotometer and AAS).
Learning Objectives
To introduce the students to the different application of analytical chemistry techniques in the
extraction and detection of poisons using simple and advance techniques with emphasis on safety and
good laboratory practice.
Intended Learning Outcomes (ILOs):
Successful completion of the course should lead to the following outcomes:
A. Knowledge and Understanding: Student is expected to
A1. Apply the different techniques of analytical chemistry in the extraction and detection of poisons
using simple and advance techniques with emphasis on safety and good laboratory practice.
B. Intellectual Analytical and Cognitive Skills: Student is expected to
B1. Understand the basic chemistry behind separation techniques used in toxicology laboratories.
B2. Appreciation of chromatographic and spectroscopic in toxicological analysis.
C. Subject- Specific Skills: Students is expected to
C1. Describe principles and methods of importance to and carry out toxicological analysis by
manipulating extraction conditions and instruments parameters.
C2. Practice writing experimental reports.
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C3. Have ability to conduct good laboratory practice in toxicological analysis.
C4. Be able to solve analytical problems.
C5. Laboratory skills development by direct supervision of students' experimental
work and marked
laboratory reports and orally in seminars.
D. Transferable Key Skills: Students is expected to
D1. Have the ability to conduct analysis using the available instruments and apply the techniques
learned in drugs analysis.
Teaching/Learning Methods
Teaching Method
Seminars and Discussions:
Homework and Assignments:
Projects:
Presentation:
Other:
ILO/s
Yes
Yes
N.A
N.A
Laboratory work
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Course Contents
Content
Reference
Introduction to Analytical
Toxicology
A. Safety in Lab oratory.
B. Good laboratory practice.
C. Orientation in toxicology
laboratory and calibration of
micropipette, balance and pH
meter.
D. General safety in laboratory.
A. Solvents, solutions and
Solubility.
B. Buffers and solutions
preparation.
C. Preparation of drug standard
solutions and buffers.
A. Introduction to sample
preparation
B. Liquid-Liquid extraction.
C. Sample preparation + (LLE).
A. Solid Phase Extraction,
diatomaceous earth and XAD2.
B. Color test.
1. Zumdahl Chemistry, Zumdahl-fifth
edition.
2. Instant Notes in Analytical Chemistry,
D.Kealey & Haines-2002.
Week
ILO/s
1
Lecture
2
Presentation,
Discussion
2
Laboratory Manual.
1. Zumdahl Chemistry, Zumdahl-fifth
edition.
2. Instant Notes in Analytical Chemistry,
D.Kealey & Haines-2002.
Laboratory Manual.
1. Clarke's Analysis of Drugs and Poisons,
Moffatt -2004.
2. Fundamentals of analytical toxicologyflanagan-2007.
Laboratory Manual.
1. Clarke's Analysis of Drugs and Poisons,
Moffatt -2004.
2. Fundamentals of analytical toxicologyflanagan-2007.
C. Solid phase extraction (SPE).
Laboratory Manual.
3
3
4
4
5
5
D. Color test.
A. Introduction to
chromatographic in analysis
B. Thin Layer Chromatography
(TLC).
C. Thin layer chromatography
(TLC).
A. Gas chromatography.
B. Conventional GC-detectors
(FID, NPD, ECD).
C. Thin layer chromatography
(TLC).
Mid Exam (theoretical and
practical).
1. Clarke's Analysis of Drugs and Poisons,
Moffatt -2004.
2. Fundamentals of analytical toxicologyflanagan-2007.
Laboratory Manual.
1. Clarke's Analysis of Drugs and Poisons,
Moffatt -2004.
2. Fundamentals of analytical toxicologyflanagan-2007.
Laboratory Manual.
6
6
7
7
8
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Laboratory
work
Presentation,
Discussion
Laboratory
work
Presentation,
Discussion
Laboratory
work
Presentation,
Discussion
Laboratory
work
Presentation,
Discussion
Laboratory
work
Presentation,
Discussion
Laboratory
work
A. High
Pressure
Chromatography.
Liquid
B. HPLC detectors (DAD, UV,
FLD).
Mid Exam (continue the practical
exam).
A. Chromatographic parameters.
B. Derivatization in GC and HPLC.
C. Gas Chromatography.
A. Introduction to Spectroscopy.
B. Molecular spectroscopy-I.
C. Gas Chromatography.
A. Atomic spectroscopy and ICP.
B. Spectroscopic
instrumentations.
C. High Performance Liquid
Chromatography (HPLC).
A. Mass spectroscopy (MS).
B. Tandem (MS/MS).
C. Spectrophotometer.
A. Method Validation in
chromatographic and
spectroscopic analysis.
1. Clarke's Analysis of Drugs and Poisons,
Moffatt -2004.
2. Fundamentals of analytical toxicologyflanagan-2007.
9
1. Clarke's Analysis of Drugs and Poisons,
Moffatt -2004.
2. Fundamentals of analytical toxicologyflanagan-2007.
Laboratory Manual.
1. Clarke's Analysis of Drugs and Poisons,
Moffatt -2004.
2. Fundamentals of analytical toxicologyflanagan-2007.
B. FPIA, EMIT and ELISA.
1. Clarke's Analysis of Drugs and Poisons,
Moffatt -2004.
2. Fundamentals of analytical toxicologyflanagan-2007.
Laboratory Manual.
1. Clarke's Analysis of Drugs and Poisons,
Moffatt -2004.
2. Fundamentals of analytical toxicologyflanagan-2007.
Laboratory Manual.
1. Clarke's Analysis of Drugs and Poisons,
Moffatt -2004.
2. Fundamentals of analytical toxicologyflanagan-2007.
B. Discussion.
10
11
1. Clarke's Analysis of Drugs and Poisons,
Moffatt -2004.
2. Fundamentals of analytical toxicologyflanagan-2007.
12
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Laboratory
work
Presentation,
Discussion
Laboratory
work
Presentation,
Discussion
Presentation,
Discussion
12
Laboratory
work
13
Presentation,
Discussion
13
Laboratory
work
Presentation,
Discussion
14
15
15
1. Clarke's Analysis of Drugs and Poisons,
Moffatt -2004.
2. Fundamentals of analytical toxicologyflanagan-2007.
Presentation,
Discussion
Laboratory
work
14
C. Final Exam (Practical Exam).
A. RIA, EIA and FIA.
10
Laboratory Manual.
B. Final Exam (Practical Exam).
A. Introduction to Immunoassay.
9
16
Laboratory
work
Presentation,
Discussion
Laboratory
work
Presentation,
Discussion
Learning Methodology:
 Seminars, Discussions, Home work and Laboratory work.
Home work and Assignments:
 Analysis of solid and liquid samples for the presence of drugs.
 Writing technical report.
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Evaluation:
Evaluation
Point %
Date
Midterm Exam
Theoretical Exam: 25%.
Practical Exam: 5%.
As
given
by The
university of Jordan
Assignments
Seminar: 10%.
Writing technical report: 10%.
During the semester.
Homework
Theoretical
and
Practical During the semester.
Homework: 10%
Theoretical Exam: 30%.
As
given
by The
Practical Exam: 10%.
university of Jordan
Final Exam
Main Reference(s):
1. Clarke's Analysis of Drugs and Poisons, Moffatt -2004.
2. Fundamentals of analytical toxicology-flanagan-2007.
References:
3. Instant Notes in Analytical Chemistry, D.Kealey & P.J. Haines-2002.
4. Zumdahl Chemistry, Zumdahl-fifth edition.
5. Internet.
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Intended Grading Scale
00-55
56-60
61-65
66-70
71-75
76-80
81-100
C
C+
BB
B+
AA
Notes:


Concerns or complaints should be expressed in the first instance to lecturer; if no resolution is
forthcoming, then the issue should be brought to the attention of the Department Chair and if still
unresolved the Dean and then ultimately the Vice President. For final complaints, there will be a
committee to review grading the final exam.
For more details on University regulations please visit: http://www.ju.edu.jo/rules/index.htm
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