Supplementary Material
Article title:
Spatial change of bacterial community in high-altitude freshwater wetland sediment
Journal name：
Limnology
Author names：
Yong Liua,b, Jingxu Zhangb, Zhao Leia, Xiaoling Zhangb, Shuguang Xieb,*
a
Yunnan Key Laboratory of Pollution Process and Management of Plateau
Lake-Watershed, Kunming 650034, China
b
College of Environmental Sciences and Engineering, The Key Laboratory of Water
and Sediment Sciences (Ministry of Education), Peking University, Beijing 100871,
China
* Corresponding author. Tel: 86-10-62751923. Fax: 86-10-62751923.
Email: xiesg@pku.edu.cn
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Table S1 Chemical features of wetland sediment samples. Samples AU and AL, BU
and BL, CU and CL, and DU and DL represent the upper layer and lower layer
sediments in sampling sites A–D, respectively. TN, total nitrogen; TP, total
phosphorous; AP, available phosphorus; TOC, total organic carbon. TOC, NH4+-N,
and TN were determined according to the literature (Song et al. 2012). TP and AP
were quantified as previously described (Hu et al. 2013).
Parameter
AU
AL
BU
BL
CU
CL
DU
DL
TN (g/kg)
1.86
0.74
1.57
0.40
0.22
0.93
0.76
2.44
NH4+-N (g/kg)
0.36
0.24
0.26
0.13
0.11
0.31
0.17
0.25
TP (g/kg)
0.64
0.63
0.55
0.50
0.73
0.39
0.43
0.25
AP (g/kg)
0.05
0.03
0.02
0.01
0.02
0.01
0.02
0.01
TOC (g/kg)
6.17
3.86
6.30
2.55
2.01
4.99
3.03
6.83
References
Song H, Li Z, Du B, Wang G, Ding Y (2012) Bacterial communities in sediments of
the shallow Lake Dongping in China. J Appl Microbiol 112(1):79–89
Hu ZF, Gao M, Xie DT, Wang ZF (2013) Phosphorus loss from dry sloping lands of
Three Gorges Reservoir area, China. Pedosphere 23:385-394
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Fig. S1 Rarefaction curves of OTUs in bacterial clone libraries. Chimera-free
sequences with ≥ 98% similarity were clustered as the same operational taxonomic
units (OTUs), and OTU-based rarefaction curves and coverage index were obtained
using DOTUR program (Schloss and Handelsman 2005).
80
AU
AL
BU
BL
CU
CL
DU
DL
70
Number of OTUs
60
50
40
30
20
10
0
0
10
20
30
40
Number of sequences
50
60
70
Reference
Schloss PD, Handelsman J (2005) Introducing DOTUR, a computer program for
defining operational taxonomic units and estimating species richness. Appl Environ
Microbiol 71(3):1501–1506
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