STANDARD OPERATING PROCEDURES FOR HAZARDOUS AND PARTICULARLY HAZARDOUS
CHEMICALS
For
BUSULFAN
(ASAF# 4147)
1. PROCEDURE /
Busulfan solution is administered to mice via intraperitoneal injection at a dose
PROCES
of 25 - 60 mg/kg of body weight. For doses above 30 mg/kg a split dose
separated by 12 hours will be used. Busulfan is dissolved in DMSO and diluted
with RPMI-1640.
2. CHEMICAL NAME(S)
Busulfan, CAS 55-98-1, is also known as 1,4-Butanediol
and associated
Dimethanesulfonate, Busilvex®, Myleran®.
PHYSICAL and
HEALTH
Warning! Carcinogen. Causes eye, skin, and respiratory tract
HAZARDS
irritation. This substance has caused adverse reproductive and fetal
effects in animals. Moisture sensitive.
Target Organs: Respiratory system, eyes, skin.
3. NAME OF TRAINER /
RESOURCE
PERSON
4. LOCATION OF
HEALTH & SAFETY
INFORMATION
Toxicity data: WISHA TWA: 0.03 mg/m3
LD 50: Mouse, IP = 86 mg/kg, Mouse, oral: = 110 mg/kg.
Principal Investigator:
Grant Trobridge – Office: SPBS 319, Phone: 368-6564
Animal Care Supervisor:
or Russ Matheson – Phone: 358-7858 (Spokane Vivarium)
The MSDS for Busulfan is kept and available in SPBS 330 suite.
Labeling: Containers with Busulfan shall either have original warning label
affixed or a label identifying the contents and hazards.
Animal Cages:
For the first two weeks or fourteen days post administration:
Signs or cards shall be placed on the mouse cages with mice treated with
Busulfan which have the appropriate hazard warning symbol and/or state:
“Caution: these mice treated with Busulfan.”
After cage is dumped following two weeks or fourteen days post administration:
Signs or cards shall be placed on the mouse cages with mice treated with
Busulfan which state: “The concentration of busulfan excreted after two weeks
or fourteen days post administration is less than 0.1%, the OSHA criteria for
when a substance is considered a potential carcinogen. As of ___/___/___, the
standard precautionary measures for handling a potential carcinogen identified
in this SOP are no longer warranted.”
5.
PROTECTIVE
EQUIPMENT
Note - Bedding from animals subject to any research substance, regardless of
the concentration or risk, should not be allowed in compost. Therefore,
although contaminated bedding dumped after two weeks is not subject to
hazardous waste requirements, it still should be disposed of as standard landfill
waste instead of compost waste (see WASTE DISPOSAL PROCEDURES
below).
Concentrated Busulfan: Wear appropriate chemical resistant gloves, chemical
splash goggles, and a lab coat while handling, dispensing, or during
experimental use of Busulfan and only do so within the confines of a certified
laboratory fume hood. Wash hands thoroughly after use.
Animals treated with busulfan, their cages and bedding materials: Animals
injected with busulfan for experimental purposes shall be identified by labeling
of the cage with the date of administration. For at least two weeks or fourteen
days after administration, up until the cage is dumped after this time period (two
weeks): wear lab coat or coveralls, chemical resistant gloves and eye protection
when handling treated animals.
6. WASTE DISPOSAL
PROCEDURES
In addition, handling treated animals or dumping bedding from their cages must
be conducted within the confines of a certified biosafety cabinet or lab hood or
use of either a half mask air purifying or powered air purifying respirator (PAPR)
equipped with HEPA filters is required.
Laboratory waste: Do not allow into domestic water supply or sewer. Waste
Busulfan should be kept in an airtight compatible container that is properly
labeled.
A completed Dangerous Waste label should be attached when waste is first
added to the container. When the container is ¾ full complete an online
Chemical Collection Request (CCR) Form, and EH&S will arrange for pickup.
Animals treated with Busulfan: place carcasses in lined biohazard box and
incinerate as medical waste.
Bedding contaminated with Busulfan: contaminated bedding accumulated
during the first two weeks (fourteen days) post administration must be disposed
of as a regulated dangerous waste. Collect in compatible container labeled with
a completed Dangerous Waste label, accompanied with completed Chemical
Collection Request form, and held until picked up by EH&S.
7.
DESIGNATED AREA
INFORMATION
8. DECONTAMINATION
PROCEDURES
Contaminated bedding dumped after two weeks is not subject to hazardous
waste requirements but still should be disposed by methods which prevent
individuals handling waste from subsequent contact; double bag contaminated
bedding materials and dispose of as standard landfill waste.
Concentrated Busulfan is stored, weighed, and mixed in in SPBS 330 suite.
Busulfan in DMSO is handled in a certified fume hood prior to dilution. Only
individuals trained in the safe handling and use of Busulfan are allowed access.
Animals treated with Busulfan: treated mice are to be housed in microisolator cages and located in a secured animal room in the Wegner vivarium.
Upon Accidental Exposure: for eye or skin contact, flush eyes or skin with
copious amounts of water for fifteen minutes; for accidental inhalation or
ingestion, immediately seek medical attention and follow instructions on MSDS.
Upon accidental release: evacuate the area immediately. Any spilled
chemical must be immediately reported to EH&S for decontamination. Small
amounts of busulfan should be cleaned up as follows: while wearing approved
respiratory protection, protective outer-garments and rubber gloves, spray spill
with water mist to prevent powder from becoming airborne, absorb and remove
to appropriate containers for disposal as hazardous waste. Remove residual
activity by wiping area with dilute Chemsolv (10 ml per 500 ml) followed by
wiping area with dilute bleach solution.
9. SPECIAL STORAGE
AND HANDLING
PROCEDURES
You must be trained in the proper cleanup procedures and medically cleared
and fit tested to wear a respirator.
Keep Busulfan in a tightly closed container. Store in a cool, dry, ventilated area
away from sources of heat or ignition. Moisture sensitive may decompose
when exposed to moist air or water. Protect against physical damage. Store
away from strong oxidizing agents.
Revision Date 08/15/2013