Supplementary Table 1 : Possible sources for migration in the population. Two sources
of possible migration were considered: (i) seeds and (ii) pollen. For (i), this includes all the
varieties that have been cultivated in the same plot as the population during the two preceding
years; for (ii), this includes all the varieties that were cultivated in the nearest fields during the
same year. These varieties are listed below with the number of alleles they share with the
parents of the population. Out of the new alleles observed in G10 and G15, none were present
in the four varieties.
Name of the variety
generation of
possible
contamination
number of loci with
alleles shared with
the parents (out of
21 loci)
number of missing
data (out of 21 loci)
Recital
Festival
Soissons
Thesee
G7
G7
G10
G10
16
13
15
18
5
7
3
3
Supplementary Table 2: Characteristics of the 21 microsatellite loci used in this study.
Locus
Xgwm135
Xgwm149
Xgwm181 *
Xgwm257
Xgwm260 *
Xgwm261
Xgwm272 *
Xgwm312 *
Xgwm325
Xgwm400
Xgwm408 *
Xgwm413
Xgwm415
Xgwm427 *
Xgwm437 *
Xgwm480
Xgwm626
Xgwm642 *
Xgwm664
Cfd71_D *
Cfd71_A
NPA
6
3
6
2
5
5
4
5
4
6
2
6
3
5
8
2
3
3
2
5
2
NNA
0
0
4
0
4
0
1
4
0
0
3
0
0
2
2
0
0
1
0
5
0
M
GA
GA
GA
GT
GA
CT
CA
GA
CT
CA
CA TA
GA
GA
CA CA
CT
CT CA
CT GT
GT
GA
CA GA
CA GA
MT
S
S
S
S
S
S
S
S
S
S
C
S
S
C
S
C
C
S
S
C
C
MP
P
P
P
P
P
P
I
P
P
P
P
P
I
P
P
P
P
I
P
P
P
NRb
20
23
28
30
20
21
17
37
16
21
22
18
25
31
24
16
13
14
22
30
30
GGP
0.13
0.29
0.91
0.39
0.00
0.68
1
0.35
0.32
0.99
0.57
0.04
0.61
0.82
0.19
0.56
0.48
0.50
0.20
.
.
GL
A
B
B
B
A
D
D
A
D
B
B
B
A
A
D
A
B
D
D
D
A
HCab NACab
0.454
19
0.440
9
0.659
16
0.567
5
0.743
18
0.553
14
0.612
6
0.761
22
0.606
10
0.685
17
0.710
18
0.711
15
0.641
8
0.811
18
0.645
20
0.215
11
0.387
5
0.449
6
0.118
3
0.778
18
0.386
7
NPA: number of parental alleles; NNA: number of new alleles; MT: motif type with simple
(repetition of a single motif; S) or complex (C) motif; MP (information available for the
Chinese Spring genotype, Roder et al. 1998): motif perfection with perfect (P) or imperfect (I)
motif; NR: number of repeats as defined in Chinese Spring (Röder et al, 1998); GGP:
GrainGenes position (relative distance between the locus and the centromere calculated from
the ITMI map GrainGenes database: http://www.graingenes.org/cgibin/ace/search/graingenes); GL: genome location; HC: Nei polymorphism index in a wheat
collection of 559 accessions (Roussel et al. 2004); NAC: number of alleles in the wheat
collection. Characteristics of microsatellites with a significant effect on the number of
parental alleles and/or the number of new alleles are marked with exponent a and/or b
respectively. Loci with new alleles are annotated.
Supplementary Figure1 : Multiple sequence alignment of three alleles of the population
and one allele of Aegilops tauschii obtained with the microsatellite locus Xgwm642.
Xgwm642-C
Xgwm642-M
Xgwm642-Z
Xgwm642-Ae.t
....|....|....|....|....|....|....|....|....|....|
5
15
25
35
45
ACGGCGAGAAGGTGCTCGCTCTTTAGTGACACTTGTGTGTGTGTGTGTGT
ACGGCGAGAAGGTGCTCGCTCTTTAGTGACACTTGTGTGTGTGTGTGTGT
ACGGCGAGAAGGTGCTCGCTCTTTAGTGACACTTGTGTGTGTGTGTGTGT
ACGGCGAGAAGGTGCTCGCTCTTTAGTGACACTTGTGTGTGTGTGTGTGT
Xgwm642-C
Xgwm642-M
Xgwm642-Z
Xgwm642-Ae.t
....|....|....|....|....|....|....|....|....|....|
55
65
75
85
95
GTGTGTGTGCGTGTGTGGCTAGAGTGCGTTGCAACAAAAGCGCAAAGTGA
GTGTGTGTGTGTGT--GGCTAGAGTGCGTTGCAACAAAAGCGCAAAGTGA
GTGTGTGTGTGT----GGCTAGAGTGCGTTGCAACAAAAGCGCAAAGTGA
GTGT------------GGCTAGAGTGCGTTGCAACAAAAGCGCAAAGTGA
Xgwm642-C
Xgwm642-M
Xgwm642-Z
Xgwm642-Ae.t
....|....|....|....|....|....|....|....|....|
105
115
125
135
145
AGGGATATGTGCTGCCGGGAAGCCCGGGGCAGTGTGGTTGG
AGGGATATGTGCTGCCGGGAAGCCCGGGGCAGTGTGGTTGG
AGGGATATGTGCTGCCGGGAAGCCCGGGGCAGTGTGGTTGG
AGGGATATGTGCTGCCGGGAAGCCCGGGGCAGTGTGGTTGG
Xgwm642-C : Parental allele Courtot (BV676500, NCBI)
Xgwm642-M : G1 new allele (BV676501, NCBI)
Xgwm642-Z : Parental allele Zenith (BV676502, NCBI)
Xgwm642-Ae.t. : Aegilops tauschii (AF497474, NCBI)
Dashes indicate gaps in the number of repeats.