Introduction to Instruments
FUJI DRI-CHEM 4000i
Scil Vet Focus 5
Cryostat for Frozen Sections
2010/12/06
Speaker：Le-Shi Tsai
Notice for the use of animal center Instrument
• Please attend illustration meeting before yo u
begin operating instruments. If you do not
participate in the illustration meeting , you may
NOT use the instrument. (The meeting will be
held 2-3 times per semester, and please pay
attention to the animal center announcement.)
• Please fill the registration forms 1 or 2 days prior
to use the instrument to facilitate preparation.
Charging methods
Instrument
Charging methods
FUJI DRI-CHEM 4000i
Biochemistry Analyzer
*Less than 3 tests :
First hour - $ 400/hour
Additional hours-$ 200 /hour
*More than 3 tests :
Each one more test, the first hour charge
extra $100
Scil Vet Focus 5
Hematology Analyzer
1 test - $750
each additional test -$100
Leica CM3050 S
Cryostat for Frozen Sections
$150/0.5hr
FUJI DRI-CHEM 4000i
Biochemistry Analyzer
Need to purchase reagents by user
Main instrument
1. The registration
forms
2. Exception code
table
Colorimetric
Potentiometric
Linear
Measurement time
External Structure of the Instrument
Light source
廢料盒
Can not open the lid when indication light is on
Internal Structure of the Instrument
Automatic detection
1. can be automatically
diluted
2. A test only needs at
least 10μL.
（ Manual detection ）
Principle：Colorimetric
Spreading layer：
1.filtered out macromolecules
(proteins, dyes, etc.)
2.evenly spread the fluid sample
into the reaction layer
β-D-glucose＋O2＋H2O GOD
H2O2
D-gluconic acid＋
1,7-Dihydroxynaphthalene＋4-aminoantipyrine＋H2O2
POD Red dye
505nm
reflection density
calibration curve
glucose concentration
Principle：Potentiometric
generated voltage difference
between ion selective electrodes
applied to a reference solution
and to the sample
potentiometric difference
between the sample and the
reference solution is measured
E = E0 + 2.303 * RT/Nf * Log ai
ai = f * Ci
Ci = Ion concentration
Scil Vet Focus 5
Hematology Analyzer
Introduction to Instrument
• The following animals can be detected:
dog、 rat、 pig、rabbit、mouse、
G. pig
• The following items can be detected:
WBC、DC、RBC、HGB、HCT、MCV、
MCH、MCHC、RDW、Retics、PLT、
MPV、PCT
Slide 002
Equipment
the registration forms
the kit
printer
waste pail
main Instrument
Equipment
the kit
1.Diluent
2.Clean Deproteinizer
3.Cellyse
1. red blood cell lysis
2. reagent to convert all hemoglobin
to the stable cyanmethemoglobin form
Principles
1. A distinguishing feature of blood cell types is
the diameter of the cells.（RBC、WBC、Platelet）
2. WBCs are classified according to the
characteristics of their cytoplasm and nucleus.
3. Cyanmethemoglobin can then be detected by
a spectrophotometer set at 540 nm. The
concentration of hemoglobin is then calculated
from the optical density of the solution.
The cells are forced to pass through
aperture in single file (one by one).
Cells are injected into the core of a
sheath flow and confined to a narrow
single-file stream by hydrodynamic
focusing(15μm).
Diluent form a vortex
感
應
區
Specimen preparation
Specimen
requirements
1. Use the EDTA anticoagulant 3 ml tubes.
2. Specimen volume: At least 1.5 ml
3. Specimen volume are less than 1.5mL(at least 100 μL):
Use small test tube containing a specimen and add
EDTA as an anti-coagulant (Manual mode).
Preservation
conditions
Samples can be analyzed within 4 hour(room temperature).
Interfering
factors
Measurement results are affected by hemolysis, chyle and
clotted blood.
DO NOT use clotted blood specimen.
Automatic Mode
• open the lid
• mix the specimen well
• put your sample into the
specimen- container
housing rack
Leica CM3050 S
Cryostat for Frozen Sections
Frozen Tissue Processing
tissue process and cutting
fixing and embedding
only use O.C.T as
embedding medium
freeze and slice
Hard tissue using
a dedicated film
stain
mounting
observe it under a microscope
The advantage of Leica CM3050 S
• It is ideal for undecalcified hard tissue :
1. can be set to any section thickness and
section automatically
2. with a dedicated film, can avoid the hard
tissue broken.
• with rough-cut function
• adjustable specimen temperature：
In accordance with the type of specimen to
select the appropriate temperature.
• adjustable cutting speed
Specifications
Specimen size
Maximum 40mm
Section thickness setting
0.5~300μm
0.1~210mm/s
Sectioning speed
Specimen temperature
-10℃~-50 ℃
supplies you need to purchase
Consumables
Suitable specimens
Stage
Buy different size according to
the size of the specimen
Disposable blades General tissue
Ukrainian carbon
blades
Dedicated film
Hard tissue
Hard tissue
Thanks for your attention