LAB 304
Lecture \ 2
Learning objectives
 Recognize types of test forms.
 General feature of the test forms .
 To know the Criteria for filling the forms.
 Analyze test forms.
 List the specimen types.
 Pre and Post- specimen collection check.
Test requisition
 Test requisition : is the request by a competent authority made to
laboratory for performing test which is needed for the diagnosis or
prognosis of the patient .
 Types of test forms :
1- hematology form
2- Chemistry form
3- Serology & virology form
4- Culture & sensitivity form
5- Hormones form
6- Urine analysis form
7- Stool analysis form
8- Multipurpose form
General feature of the test forms
 Every form should include the following :
1- general information : related to the patient & the ward
2- hospital name
3- form’s type
4- clinical diagnosis
5- Processing type
6- date , time & name of the sender
7- tests to be done
8- analyzer information
Patient &
ward info.
Hospital
name
Order
information
Processing
type
diagnosis
Form
type
Test to
be
done
Lab
information
Test requisition
 Criteria for filling the forms :
1- patient’s name, age, sex, nationality & hospital ( file ) number.
2- relevant ward details .
3- name of ( consultant / specialist )
4- highlight the tests to be done .
5- request must be signed and stamped by the requesting
physician .
6- the staff nurse collecting the sample must ensure labeling of
the sample & fill the request form including time & date .
Missing
DR. Stamp
ICU,NICU,PICU,MMW,
MSW,FMW,FSW,MSP
W,OBW,LRDR,OR,HD
U,NSY,ER
Clinical
diagnosis
Ordered
test
Processing
type
Lab no.
Test requisition
 The requesting physician must check following before
ordering :
1- availability of test in the lab .
2- inform the patient about the test procedure and any
precaution to be taken .
3- instruct the staff nurse about any precautions to be taken
during collection .
HAEMTOLOGY FORM
 Consist of these tests :
1- CBC
2- Differential count
3- RBC morphology
4- Blood parasite
5- ESR
6- Sickle cell test
7-Reticulocyte cell account
U/E
LFT
Cardiac
enzyme
Lipid
profile
SEROLOGY FORM ABB.
 ASO: Antistreptolysin O Titer(streptococcal infection)
 VDRL : Venereal disease research laboratory (syphilis
caused by Treponema pallidum )
 TPHA : Treponema Pallidum Haemagglutination Assay
(syphilis caused by Treponema pallidum )
 Widal test : (typhoid fever )
 TOXO : Toxoplasmosis (Toxoplasma gondii parasite )
HORMONES FORM









T3 : Triiodothyronine
T4 : Thyroxine
TSH : Thyroid-stimulating Hormone
PTH : Parathyroid Hormone
FSH : Follicle-stimulating Hormone
LH : Luteinizing hormone
PRL : Prolactin
βHCG : Human Chorionic Gonadotropin ( preg. Test )
ACTH : Adrenocorticotropic Hormone (excessive or
deficient cortisol in the body )
STOOL ANALYSIS FORM ABB.
 E.histo .veg. : Entamoeba histolytica vegetative(trophozoite)
 E. histo. Cyst : Entamoeba histolytica cyst
 E. coli veg. : Entamoeba coli vegetative
 E. coli cyst : Entamoeba coli cyst
 G. lamblia : Giardia lamblia
 T. hominis : Trichomonas hominis
 C. mesnili : Chilomastix mesnili
STOOL ANALYSIS FORM ABB.
 Trictric : Trichuris trichura
 S. mansoni : Schistosoma mansoni
 T. saginata : Taenia saginata
 H. nana : Hymenolepis nana
Specimen collection
 Phlebotomist : the person who trained to enters a vein
through needle to extract blood .
 Types of specimen :
1- blood
7- body fluids
2- sputum
8- throat swab
3- CSF
9- ear swab
4- fecal
10- eye swab
5- urine
11- mouth swab
6- semen
12- nose swab
13- vaginal swab
14- abscesses &
wound swab
Specimen collection
 Specimen collection is the first step in proficiency
surveillance. For issuing accurate and precise results, it is
the job of the nurse or phlebotomist to make sure that
the enumerated conditions are met and implemented in
order to deliver a suitable sample for analysis so that an
accurate and precise result will be issued.
Procedure of specimen collection
 Pre-collection check :
1- review the request and identify the patient by asking the
name, seeing his ID and compare it with the request form.
2- Is there any special instruction / preparation needed for the
patient. If so advice the patient accordingly
e.g. fasting or after meals and sample collection . container
may be given if needed e.g. 24 hour urine collection or
sputum collection.
Procedure of specimen collection
3- Read all the tests and select the tubes/containers as
needed for the tests.
4- Arrange the tubes in order of filling.
Procedure of specimen collection
 Post collection :
1- Label all the tubes and specimen bottles as per the
guideline.
2- NEVER write the name before extraction and NEVER ask
any other person to write for the same.
3- Finish the job before patient leaves the chair .