Presentation - R&D
CavinKare Research Centre
CavinKare Pvt. Ltd.
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Objectives of R&D
• To contribute to the business objectives
of CKPL by developing innovative, cost
effective and superior products and
processes
• To make R&D a self-supporting / profit
making activity by creating and
developing Intellectual Property through
out-licensing or national / international
collaborations
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Product Portfolio
Personal Care : Hair, Skin and Home
Shampoo
Hair Wash Powder
Hair Dye
Hair Oil
Cream / Lotion
Talc, Deo & Dab-on
Toilet Bowl Cleaner
Mosquito Coil
Chik, Nyle, Meera, Karthika
Meera, Karthika
Indica
Meera, Nyle
Fairever, FE Mantra, Nyle CC & ML
Spinz
Tex
Dishoom
Foods : Ready-to-eat
Pickles
Spices
Vermicelli
Chinni’s, Ruchi
Chinni’s
Chinni’s
Confidential Document : Access to Approved Users Only
Sweets
Ruchi
Dry Fruits
Snack Bar
Chinni’s Moments
Chinni’s Cruncho
R&D - Relationships
Personal Care (Domestic Business)
Foods (Domestic Business)
International Business
Salon Business (Trends in Vogue)
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R&D Strategy for Competitive Advantage
To Provide Superior Products at Affordable Price Points
• Superior Formulation (sensory experience; delivery of benefit; care)
• Discovery of new ingredients (exclusivity; superior performance)
• Developing new / improved processes (cost effectiveness)
• Developing new technology (exclusivity; superior performance)
• Strengthening biology (developing capability; studying new pathways)
• Leveraging IPR (drafting claims; analysis; competitive intelligence)
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R&D Set-up & Facilities
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CRC at a Glance
Buildings = 2
Total Build-up Area = 30000 Ft2
Annual Expenditure
Personnel Strength
= @1% of Turnover
= 40 (Projected 2007)
(Technical)
Educational
Status
Personnel Status
(As on Aug 07)
Doctorates
8
Masters
24
Graduates
4
Total
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Number
36
Major Equipments
(Analytical)
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Gas Chromatograph
HPLC
TLC Scanner
UV Spectrophotometers
Moisture analyser (Infrared, Karl-Fischer)
Viscometer
Mexameter
Skin Testing Equipment
(sebum level, moisture, pH, elasticity, TEWL)
• Hair Testing Equipment
(tensile prop., combability, volume)
• Reflection Spectrophotometer
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Major Equipments
(Infrastructural)
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Stability chambers
Rotary evaporator
Clean air workbench
Cold room
Ultra-low Temp Fridge (-70°C)
Washing Machine
Soxhlet extractor
Autoclave
Tray Driers
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Cell culture facility
Tergetometer
Triple Roll Mill
Contra-Rotary Mixer
Sigma Mixer
Simplex Plodder
Ribbon Mixer
Horizontal Mixer
Mini Soap Plodder
Rotary coating pan
• Generator Backup
• Effluent Treatment Plant
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Information / Knowledge Management
Information Systems
• A fully networked facility with a central control (server)
based on ‘thin client’ technology and a Document
Management System
• Sample Tracking for all analytical operations
including work flow
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R&D Programme
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R&D Programme : Basic Research
Extraction, isolation and characterisation of bioactive / functional
compounds from natural sources
Synthesis, characterisation and new process development of
ingredients for personal care products
Screening of natural products for microbiocidal activities with an
emphasis on preservation of products and functional delivery
(e.g. antidandruff)
Characterisation of major ingredients presently used in personal
care products to optimise their performance
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R&D Programme : Product Development
Development of Formulations and Processes for Safe & Effective
Products with emphasis on functional delivery in the areas of  Hair Care Products
 Skin Care Products
 Cleaning and Hygiene Products
Continuous optimisation and development of new formulation
to keep the product cost under control
Development of Spices, Vermicelli and Ready-to-Eat Products
Such as Pickles, Nut based products (like peanuts, cashew),
Snack foods and Traditional Sweets
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Outline of R&D Activities
Confidential Document : Access to Approved Users Only
Product Development
(Selection of Ingredients)
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Focus on Benefits
Delivery of Promise (Claims substantiation)
Superior Sensory Experience
Gentle / Safe
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Product Development : Shampoo
Typical Ingredients
Surfactants
Foam Boosters
Conditioning Additives
Viscosity Modifiers
Opacifiers
Pearlizers
Preservatives
pH modifiers
Perfume
Colour
Confidential Document : Access to Approved Users Only
Typical Analysis
Appearance / Colour
Perfume note
pH
Active Detergent
Viscosity in cps
Foam Height in cms
Salt Content
Microbial Test
Sensory Evaluation for Products
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Product Support
• Identifying / Developing New Ingredients
(biochemistry, microbiology & chemistry)
• Developing Specifications & Methods (Measurement)
• Developing New Processes (Chemistry)
• Demonstrating Efficacy (Biochemistry, Claim substantiation)
• Facing the Challenge of Contamination (Microbiology)
• Information / Knowledge Management (Library)
• Facilitating Activities (IT services, administration etc)
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Claim Substantiation
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Safety evaluation of Products e.g. Irritancy test
Developing study protocol
Identifying clinical investigator
Preparing documents like
Consent Form,
Investigator Declaration Form,
Product Brief,
Assessment Form etc
• Recruitment of volunteers
• Coordination and conduct of the study
• Data collation, Analysis and Report submission
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Hair Oil - Study Highlights
(Claim Substantiation)
Graph 1 - Hair Fall Reduction in Volunteers After Use of Meera
Oil and Parachute Coconut Oil
Graph 2 - Reduction in Hair Root Involved Hair Fall in
Volunteers After Use of Meera Oil and Parachute Coconut Oil
521
501
500
477
432
427
400
327
300
200
152
101
100
0
0
15
30
45
60
Days after oil use
Meera Oil Users
75
350
No. of hairs with root
No. of hairs obtained
600
225
200
150
103
100
50
90
0
2
0
0
15
30
45
60
75
90
Days after use
Parachute Coconut Oil Users
90
285
265
284
250
Meera Oil Users
Graph 3 - Increase in Anagen Hairs in Volunteers After
Use of Meera Oil and Parachute Coconut Oil
Parachute Coconut Oil Users
Chart 1 - Rating of Volunteers on Product Efficacy in
Reducing Hair Fall
100
79
69
75
60
37
45
30
22
22
20
19
15
15
% of volunteers
% of anagen hairs
309
300
80
60
40
20
0
0
0
30
Meera Oil Users
Days after use
60
Parachute Coconut Oil Users
90
Poor
Meera Oil Users
Moderate
Good
Rating Coconut Oil Users
Parachute
Note : No. of hairs obtained is the number of hair collected during combing in the clinic
Confidential Document : Access to Approved Users Only
Evaluating a Skin Lightening Cream
(Claim Substantiation)
Assessing Reduction in Pigmentation
2000
1500
Volunteer Perception of Efficacy
1000
16
500
0
0
1
2
3
4
Weeks
Reduction in Average Mexameter Reading
% Reduction
= 248
= 13.95 %
Number of Volunteers
Mexameter Reading
2500
15
14
12
10
10
8
6
4
2
3
1
0
Poor
Moderate
Rating
Confidential Document : Access to Approved Users Only
Good
Excellent
Biochemical Assays
Mildness Assay (Shampoo) 1. Zein assay
2. Collagen swelling
3. pH rise
Skin Lightening Assay
1. Tyrosinase inhibition assay
2. DOPA autooxidation inhibition assay
Anti-aging Assay
Elastase inhibition asssay
Antiinflammatory Assay Soyabean lipooxygenase inhibition assay
Antioxidant Assay
Confidential Document : Access to Approved Users Only
1. DPPH radical scavenging assay
2. Deoxyribose degradation assay
3. Superoxide Dismutase assay
Cell Based Assays
Skin Lightening
1. Melanin assay
2. Tyrosinase inhibition assay
Oxidative stress
Cytotoxicity Assay
Cytotoxicity / Proliferation
MTT assay
Mildness Assay (Shampoo)
1. Cytotoxicity assay
2. RBC lysis assay
Cell lines Available :
Melanocyte (melanoma)
Fibroblast (normal)
Keratinocyte (carcinoma)
General (cancer)
Primary cell lines
Confidential Document : Access to Approved Users Only
B16-F10, B16-F1 & G361
NIH / 3T3
A431
HeLa
Fibroblasts & Keratinocytes
Chemistry
Extraction, isolation and characterisation of bioactive / functional
Compounds from natural sources (skin lightening, antioxidants, antiaging etc.)
Synthesis, characterisation and new process development of
Ingredients for personal care products
Screening of natural products for microbiocidal activities with an
Emphasis on preservation of products and functional delivery
(e.g. antidandruff)
Development of natural hair colours
Confidential Document : Access to Approved Users Only
RCOOCH2
Chemical Process
1.
Oleochemicals
KOH
RCOOCH + 3 HOC2H4NH2
130-40 oC
RCOOCH2
Glyceride
ester (CNO)
Secondary Surfactants
1. Monoethanol amides
2. Amidopropyl betaines
MEA
Monoethanol amide
130-35oC
2.
3.
RCO-NH C2H4OH + MeOH
RCOOCH3 + HOC2H4NH2
Fatty acid ester
Also useful as Thickening Agent
KOH
MEA
RCOOH + HOC2H4NH2
Fatty acid
176oC
KOH
MEA
RCOOCH2
1a
RCOOCH + 3 N(CH2)3NH2
KOH
3 RCO-NH (CH2)3N
130-40 oC
+ Glycerin
RCOOCH2
Glyceride
ester
DAPA
Amidoamine
80-90C
1b
RCO-NH (CH2)3N
Amidoamine
2a
R-COOH / +
RCOOMe
3 RCO-NH (CH2)3N-CH2COO + NaCl
+ ClCH2COONa
SMCA
N(CH2)3NH2
3 RCO-NH C2H4OH + Glycerin
Cocoamidoproply betaine
KOH
o
RCO-NH (CH2)3N
130-40 C
Fatty acid/
DAPA
Methyl ester
Confidential Document : Access to Approved Users Only
Amidoamine
+ H2O/ MeOH
Monoethanolamide
RCO-NH C2H4OH + H2O
Monoethanol amide
Microbiology
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Study of anti-microbial efficacy (MIC, synergy etc)
Selection of suitable preservatives for products
Estimation of microbial load during development
Preservative Challenge Test
Screening natural products for antidandruff activity
Evaluation of competitive products
Kill Kinetics of a Fungal Test Strain by an Antifungal
Log CFU
7
6
5
4
3
2
0.25x
0.5x
1x
4x
Control
1
0
0
5
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10
15
Time (h)
20
25
30
Measurements
Total No. Assays
= >190
Major Responsibilities
• Analysis of Own Products
• Analysis of Competitor Products
• Setting up Specifications
• Interfacing with Regulatory Authorities
(BIS, Drug Controllers)
Sample
Tracking
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