Introducing HALO-5
The World’s First
Ultra-High-Performance
5-Micron Packing
HALO-5 provides the efficiency of
3-micron particles with the
pressure of 5-micron particles
HALO® -5 Fused-Core Particles
~ 5 µm
0.6 µm
SEM of ~ 5 µm HALO Fused-core
Graphical representation of ~ 5 µm HALO Fused-core
Specifications for
HALO-5 Particles






~ 5 μm overall diameter
0.6-μm-thick porous outer shell
90 Angstrom pores in porous shell
Highly purified Type B silica
Surface area ~100 m2/g
Stable at pressures of at least 600 bar
HALO-5 Bonded Phases
Available November 2012
- Phenyl-Hexyl
- C8
C18 (octadecyl)
- ES-CN
Available February 2013
PFP
(pentafluorophenylpropyl)
- HILIC
-Penta-HILIC
More Theoretical Plates at Lower Pressure
5 µm HALO fused-core
4 µm HALO fused-core
5 µm totally porous
Plates/Pressure in bar
2.7 µm HALO fused-core
3 µm totally porous
1.8 µm totally porous
0
100
200
300
400
• When operated at optimum flow rates, HALO-5 columns provide the
highest number of theoretical plates per unit of pressure
HALO-5 Part Numbers
Part Number
95812-202
95812-302
95812-402
95812-502
95812-602
95812-702
95812-902
95813-202
95813-302
95813-402
95813-502
95813-602
95813-702
95813-902
95814-202
95814-302
95814-402
95814-502
95814-602
95814-702
95814-902
95812-209
95812-309
95812-409
95812-509
95812-609
95812-709
95812-909
Description
5.0um Halo C18
5.0um Halo C18
5.0um Halo C18
5.0um Halo C18
5.0um Halo C18
5.0um Halo C18
5.0um Halo C18
5.0um Halo C18
5.0um Halo C18
5.0um Halo C18
5.0um Halo C18
5.0um Halo C18
5.0um Halo C18
5.0um Halo C18
5.0um Halo C18
5.0um Halo C18
5.0um Halo C18
5.0um Halo C18
5.0um Halo C18
5.0um Halo C18
5.0um Halo C18
5.0um Halo PFP
5.0um Halo PFP
5.0um Halo PFP
5.0um Halo PFP
5.0um Halo PFP
5.0um Halo PFP
5.0um Halo PFP
2.1 x 20mm
2.1 x 30mm
2.1 x 50mm
2.1 x 75mm
2.1 x 100mm
2.1 x 150mm
2.1 x 250mm
3.0 x 20mm
3.0 x 30mm
3.0 x 50mm
3.0 x 75mm
3.0 x 100mm
3.0 x 150mm
3.0 x 250mm
4.6 x 20mm
4.6 x 30mm
4.6 x 50mm
4.6 x 75mm
4.6 x 100mm
4.6 x 150mm
4.6 x 250mm
2.1 x 20mm
2.1 x 30mm
2.1 x 50mm
2.1 x 75mm
2.1 x 100mm
2.1 x 150mm
2.1 x 250mm
Part Number
95813-209
95813-309
95813-409
95813-509
95813-609
95813-709
95813-909
95814-209
95814-309
95814-409
95814-509
95814-609
95814-709
95814-909
95812-102
x 5mm, 3 pk
95813-102
x 5mm, 3 pk
95814-102
x 5mm, 3 pk
95812-109
x 5mm, 3 pk
95813-109
x 5mm, 3 pk
95814-109
x 5mm, 3 pk
Description
5.0um Halo PFP 3.0 x 20mm
5.0um Halo PFP 3.0 x 30mm
5.0um Halo PFP 3.0 x 50mm
5.0um Halo PFP 3.0 x 75mm
5.0um Halo PFP 3.0 x 100mm
5.0um Halo PFP 3.0 x 150mm
5.0um Halo PFP 3.0 x 250mm
5.0um Halo PFP 4.6 x 20mm
5.0um Halo PFP 4.6 x 30mm
5.0um Halo PFP 4.6 x 50mm
5.0um Halo PFP 4.6 x 75mm
5.0um Halo PFP 4.6 x 100mm
5.0um Halo PFP 4.6 x 150mm
5.0um Halo PFP 4.6 x 250mm
5.0um Halo C18 Guard Column, 2.1
5.0um Halo C18 Guard Column, 3.0
5.0um Halo C18 Guard Column, 4.6
5.0um Halo PFP Guard Column, 2.1
5.0um Halo PFP Guard Column, 3.0
5.0um Halo PFP Guard Column, 4.6
HALO-5 Columns Exhibit the Same Sample Loadability as 2.7-Micron
HALO Columns and are Similar to 5-Micron Totally Porous Columns
35000
30000
25000
Plate Number
2.7 µm Fused-Core, 0.5 µm shell
20000
5 µm Fused-Core, 0.6 µm shell
5 µm Totally porous
15000
10000
5000
0.01
0.1
1
µg of 1-Cl-4-Nitrobenzene
10
100
Easy Transfer from Existing Methods
• HALO-5 Columns Can be Substituted for 5-Micron Totally
Porous Columns to Provide Sharper Peaks and Shorter Analysis
Times Under Identical Method Parameters
Obtain 5-Micron Benefits for 3-Micron Methods
• HALO-5 Columns Provide the Same Efficiency as 3-Micron
Columns at about Half the Pressure
Comparable Selectivity with 2.7-Micron HALO
PEAK IDENTITIES (in order):
1. Uracil
2. Resorcinol
3. Aniline
4. 4-Chloroaniline
5. Acetoacetanilide
6. Dimethylphthalate
7. Cinnamyl alcohol
8. 2,6-Dinitrotoluene
9. Tolbutamide
10. 4-Chloro-3-nitroanisole
• Methods are easily transferred from HALO 2.7-micron
columns to HALO-5 Columns with only minor adjustment
to mobile phase strength
Who Should Use HALO-5 Columns?
 Researchers who currently use 3- or 5-micron
particle columns and want to increase their
efficiency and speed
 Anyone wanting to transfer a UHPLC method
to a routine laboratory
 Users who want more speed or who want
more separation power but are owners of
pressure-limited HPLC instruments (400 bar
max pressure)
Conclusions
Regarding HALO-5 Columns
 Produce unusual column efficiency; reduced plate
heights h of ~1.2 for small molecules making them the
first high-performance 5-micron columns in the world.
 Efficiency and separation speed rivals that of
3-μm columns, but with about one-half the pressure.
 Thin porous outer shell results in superior mass
transfer kinetics and better efficiency at high mobile
phase velocities, especially for larger molecules.
 2-μm porosity frits allow the convenience and
ruggedness of columns with 5 μm particles.
 Strong particles allow pressures of at least 600 bar.