Cell Organelles Chapter 7, page 174 Modified from an original PPT of Beauchemin 2006 Introduction to Cells Inside a Cell What is a cell? A cell is a collection of living matter enclosed by a barrier that separates the cell from its surroundings. The cell is the basic unit of all forms of life. Remember the three parts to cell theory: 1. All living organisms are composed of one or more cells. 2. The cell is the basic unit of structure, function in all organisms. 3. All cells come from preexisting, living cells. (An organism is a living thing.) There are two main categories of cells: Prokaryote (prokaryotic cells) Bacteria Have no membrane-bound nucleus Nucleic acid is usually found in “loops” in the cytoplasm Usually smaller than eukaryotes Have fewer organelles than eukaryotes Eukaryotes (eukaryotic cells) Larger Have a membrane-bound nucleus where DNA is located More organelles All organisms except bacteria are eukaryotes Homework Read pages 174 to 181 Answer questions 1 to 4 on page 181 Be able to label both a plant and an animal cell (use your handouts for practice) Cell Organelles in Eukaryotes Organelle means “little organ” Cytoplasm is everything inside the cell membrane, except the nucleus Cytosol or the intracellular fluid is the liquid found inside cells. Cell Membrane The boundary of the cell Cell Membrane Surrounds the cytoplasm and holds the shape of the cell Protects the cell Allows certain material to pass through (selectively permeable) Phospholipid Bilayer For more information on the cell membrane, please refer to your handout. Cell Wall A tough, flexible but sometimes fairly rigid layer that surrounds some types of cells. Located outside the cell membrane Provides structural support and protection, sometimes preventing the cell from bursting from water entering the cell. Found in plants, some bacteria, fungi, and algae. Nucleus Control center of the cell Contains DNA which contains the coded instructions for making proteins which are the molecules that do “everything”. Nucleus continued Surrounded by a double membrane called the nuclear membrane or nuclear envelope. Nuclear envelope has thousands of pores which allow material to move into and out of the nucleus. Usually one per cell (red blood cells do not have nuclei.) Usually the easiest organelle to see under a microscope Chromatin in the Nucleus The thread-like material in the nucleus is called chromatin which is DNA wrapped around protein. Usually looks like this but as a cell prepares to divide, chromatin condenses to form chromosomes. Chromosomes Nucleolus Small dense region within the nucleus The assembly of ribosomes begins here Ribosome Proteins are assembled by ribosomes, following instructions from DNA. Ribosomes are found attached to rough endoplasmic reticulum or floating free in cytoplasm Produced in a part of the nucleus called the nucleolus Endoplasmic Reticulum (ER) The internal membrane system of the cell Connected to the nuclear membrane Assembles lipid components of the cell membrane, proteins and other materials for export from the cell. Rough Endoplasmic Reticulum Studded with ribosomes Proteins that are being synthesized for export from the cell and proteins that are to be used in the cell membrane are made on the these ribosomes. Proteins synthesized on the ribosomes on the RER enter the RER where they may be chemically modified. Smooth Endoplasmic Reticulum No ribosomes Synthesizes lipids Contains many enzymes which have specialized jobs Makes membrane lipids; carries proteins; detoxifies drugs Golgi Apparatus Looks like a stack of plates Modifies, sorts, and packages proteins and other materials made in the ER for storage in the cell or secretion from the cell. Golgi Apparatus (continued) Vesicles which contain the materials modified by the Golgi Apparatus pinch off the ends. Lysosomes Clean-up crew (sometimes called cannibals) Membrane-bound sacks filled with enzymes that break down lipids, carbohydrates and proteins from food into smaller molecules that the cell can use. Which organelles do lysosomes work with? Lysosomes (continued) Also break down organelles that have outlived their usefulness for disposal. Vacuoles Storage container for water, food, enzymes, wastes, pigments, etc. Large central vacuole in plant cells Many small vacuoles in animal cells What type of microscope may have been used to take this picture? Contractile Vacuole Mitochondria mitochondrion) “Powerhouse of the cell” Convert the chemical energy from food into energy that can be used by the cell. Enclosed by two membranes---an outer one and another that is folded up inside the mitochondrion (singular = Mitochondria (continued) Cellular respiration occurs here to release energy for the cell to use Has its own strand of DNA Our mitochondria are inherited from our mothers via the eggs cell. Chloroplast Found in plant cells Contains the green pigment chlorophyll which captures the sun’s energy and converts it into chemical energy (glucose) in a process called photosynthesis. Cytoskeleton A network of protein filaments/tubes that helps the cell maintain its shape and which helps move materials around the cell. Cytoskeleton (cont’d) Acts as skeleton and muscle Provides shape and structure Microfilaments (1 type of cytskeleton) Thread-like structures Made of a protein called actin Make a tough, flexible framework that supports the cell May also help the cell move by assembling and disassembling Microtubules (another type ofmicroskeleton) Hollow structures made of proteins known as tubulins Perform different functions in different cells: Critical to maintaining shape of some cells Form spindles in mitosis Form centrioles (animal cells only) Build projections from cell’s surface such as flagella and cilia that enable some cells to “swim” Some cells have them arranged so that they can be used to produce controlled movements of the cell. Centrioles Aid in cell division Found in pairs in animal cells Made of microtubules Cell Wall Found in plant and bacterial cells Rigid, protective barrier Located outside of the cell membran Made of cellulose (sugar/fiber) Quick Review Which organelle is the control center of the cell? Nucleus Which organelle holds the cell together? Cell membrane Which organelles are not found in animal cells? Cell wall, central vacuole, chloroplasts Which organelle helps plant cells make food? Chloroplasts What does E.R. stand for? Endoplasmic reticulum