The Cell Cycle and
Mitosis lab
MITOSIS LAB
Allium root tip cells
MITOSIS
LAB
Unwinding DNA
Every nucleated cell in your body
has 46 chromosomes
A Chromosome
Replication:
• Centrioles replicate
• A single-stranded
chromosome replicates
into a double-stranded
chromosome
• Double-stranded
chromosomes are joined
by centromeres
http://www.umanitoba.ca/Biology/lab3/biolab3_5.html
Allium Root tip
• Why are we looking in this region?
Allium Root tip
Allium Root tip
(A) Interphase
• Not a part of mitosis
• The cell grows,
replicates DNA and
prepares for Mitosis
Replication:
(B) Prophase
• Centrioles replicate
• A single-stranded chromosome
replicates into a double-stranded
chromosome
• Double-stranded chromosomes are
joined by centromeres
• Centrioles migrate to opposite ends
or poles of the cell forming spindle
fibers
(C)
Metaphase
(C)
Metaphase
The doublestranded
chromosomes line
up in the middle of
the cell.
(D)
Anaphase
• The double-stranded
chromosomes break at the
centromere.
• The daughter
chromosomes then move
apart to opposite poles
with the aid of spindle
fibers
(E) Telophase
• Last phase of mitosis
• Begins when chromosomes
reach the poles
• A nuclear membrane forms
• Cytokinesis (cytoplasmic
division)
– Cell “pinches in”
• Results in 2 exact daughter
cells
Citations and Special thanks
• First published Oct 98: Modified Aug 05
Copyright © Michael Shaw 2005 (Images
and Text)
• Joe Saccone Eastchester NY,
• If you feel that your information was not
sited please contact me and I will update my
cite.
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Interphase
Prophase
Metaphase
Anaphase
Telophase
Interphase