Boundless Lecture Slides Available on the Boundless Teaching Platform Free to share, print, make copies and changes. Get yours at www.boundless.com Boundless Teaching Platform Boundless empowers educators to engage their students with affordable, customizable textbooks and intuitive teaching tools. The free Boundless Teaching Platform gives educators the ability to customize textbooks in more than 20 subjects that align to hundreds of popular titles. Get started by using high quality Boundless books, or make switching to our platform easier by building from Boundless content pre-organized to match the assigned textbook. This platform gives educators the tools they need to assign readings and assessments, monitor student activity, and lead their classes with pre-made teaching resources. Using Boundless Presentations The Appendix The appendix is for you to use to add depth and breadth to your lectures. You can simply drag and drop slides from the appendix into the main presentation to make for a richer lecture experience. Get started now at: http://boundless.com/teaching-platform Free to edit, share, and copy Feel free to edit, share, and make as many copies of the Boundless presentations as you like. We encourage you to take these presentations and make them your own. If you have any questions or problems please email: educators@boundless.com Free to share, print, make copies and changes. Get yours at www.boundless.com About Boundless Boundless is an innovative technology company making education more affordable and accessible for students everywhere. The company creates the world’s best open educational content in 20+ subjects that align to more than 1,000 popular college textbooks. Boundless integrates learning technology into all its premium books to help students study more efficiently at a fraction of the cost of traditional textbooks. The company also empowers educators to engage their students more effectively through customizable books and intuitive teaching tools as part of the Boundless Teaching Platform. More than 2 million learners access Boundless free and premium content each month across the company’s wide distribution platforms, including its website, iOS apps, Kindle books, and iBooks. To get started learning or teaching with Boundless, visit boundless.com. Free to share, print, make copies and changes. Get yours at www.boundless.com Protists > Groups of Protists Groups of Protists • Excavata • Chromalveolata: Alveolates • Chromalveolata: Stramenopiles • Rhizaria • Archaeplastida • Amoebozoa and Opisthokonta Free to share, print, make copies and changes. Get yours at www.boundless.com www.boundless.com/biology Protists > Groups of Protists Excavata • Excavata are a supergroup of protists that are defined by an asymmetrical appearance with a feeding groove that is "excavated" from one side; it includes various types of organisms which are parasitic, photosynthetic and heterotrophic predators. • Excavata includes the protists: Diplomonads, Parabasalids and Euglenozoans. • Diplomonads are defined by the presence of a nonfunctional, mitochrondrialremnant organelle called a mitosome. • Parabasalids are characterized by a semi-functional mitochondria referred to as a hydrogenosome; they are comprised of parasitic protists, such as <em>Giardia lamblia </em> <em>Trichomonas vaginalis</em>. View on Boundless.com • Euglenozoans can be classified as mixotrophs, heterotrophs, autotrophs, and parasites; they are defined by their use of flagella for movement. Free to share, print, make copies and changes. Get yours at www.boundless.com www.boundless.com/biology/textbooks/boundless-biology-textbook/protists-23/groups-of-protists-147/excavata-580-11797 Protists > Groups of Protists Chromalveolata: Alveolates • Alveolates are classified under the group Chromalveolata which developed as a result of a secondary endosymbiotic event. • Dinoflagellates are defined by their flagella structure which lays perpendicular and fits into the cellulose plates of the dinoflagellate, promoting a spinning motion. • Apicomplexans are defined by the asymmetrical distribution of their microtubules, fibrin, and vacuoles; they include the parasitic protist <em>Plasmodium</em> which causes malaria. • Ciliates are defined by the presence of cilia (such as the oral groove in the <em>Paramecium)</em>, which beat synchronously to aid the organism in Dinoflagellates locomotion and obtaining nutrients. View on Boundless.com • Ciliates are defined by the presence of cilia, which beat synchronously, to aid the organism in locomotion and obtaining nutrients, such as the oral groove in the <em>Paramecium</em>. Free to share, print, make copies and changes. Get yours at www.boundless.com www.boundless.com/biology/textbooks/boundless-biology-textbook/protists-23/groups-of-protists-147/chromalveolata-alveolates-581-11798 Protists > Groups of Protists Chromalveolata: Stramenopiles • Stramenophiles, also referred to as heterokonts, are a subclass of chromalveolata, and are identified by the presence of a "hairy" flagellum. • Diatoms, present in both freshwater and marine plankton, are unicellular photosynthetic protists that are characterized by the presence of a cell wall composed of silicon dioxide that displays intricate patterns. • Golden algae, present in both freshwater and marine plankton communities, are unicellular photosynthetic protists characterized by the presence of carotenoids (yellow-orange photosynthetic pigments). • Oomycetes, commonly referred to as water molds, are characterized by their fungus-like morphology, a cellulose-based cell wall, and a filamentous network Stramenophile structure View on Boundless.com used for nutrient uptake. • Oomycetes, commonly referred to as water molds, are characterized by their fungus-like morphology, a cellulose-based cell wall and a filamentous network used for nutrient uptake. Free to share, print, make copies and changes. Get yours at www.boundless.com www.boundless.com/biology/textbooks/boundless-biology-textbook/protists-23/groups-of-protists-147/chromalveolata-stramenopiles-582-11799 Protists > Groups of Protists Rhizaria • The needle-like pseudopodia are used to carry out a process called cytoplasmic streaming which is a means of locomotion or distributing nutrients and oxygen. • Two major subclassifications of Rhizaria include Forams and Radiolarians. • Forams are characterized as unicellular heterotrophic protists that have porous shells, referred to as tests, which can contain photosynthetic algae that the foram can use as a nutrient source. • Radiolarians are characterized by a glassy silica exterior that displays either bilateral or radial symmetry. Ammonia tepida View on Boundless.com Free to share, print, make copies and changes. Get yours at www.boundless.com www.boundless.com/biology/textbooks/boundless-biology-textbook/protists-23/groups-of-protists-147/rhizaria-583-11800 Protists > Groups of Protists Archaeplastida • Archaeplastida are typically associated with their relationship to land plants; in addition, molecular evidence shows that Archaeplastida evolved from an endosymbiotic relationship between a heterotrophic protist and a cyanobacterium. • Red algae (rhodophytes), are classified as Archaeplastida and are most often characterized by the presence of the red pigment phycoerythrin; however, there are red algae that lack phycoerythrins and can be classified as parasites. • Red algae typically exist as multicellular protists that lack flagella; however, they can also exist as unicellular organisms. • Green algae are the most abundant group of algae and can be further classified as chlorophytes and charophytes. <em>Volvox aureus </em> View on Boundless.com • Charophytes are the green algae which resemble land plants and are their closest living relative. • Chlorophytes are the green algae which exhibit a wide range of forms; they can be unicellular, multicellular, or colonial. Free to share, print, make copies and changes. Get yours at www.boundless.com www.boundless.com/biology/textbooks/boundless-biology-textbook/protists-23/groups-of-protists-147/archaeplastida-584-11801 Protists > Groups of Protists Amoebozoa and Opisthokonta • Amoebozoa (amoebas) can live in either marine and fresh water or in soil. • Amoebozoa are characterized by the presence of pseudopodia, which are extensions that can be either tube-like or flat lobes and are used for locomotion and feeding. • Amooebozoa can be further divided into subclassifications that include slime molds; these can be found as both plasmodial and cellular types. • Plasmodial slime molds are characterized by the presence of large, multinucleate cells that have the ability to glide along the surface and engulf food particles as they move. Pseudopodia structures • Cellular molds are characterized by the presence of independent amoeboid cells View on Boundless.com during times of nutrient abundancy and the development of a cellular mass, called a slug, during times of nutrient depletion. • Archamoebae, Flabellinea, and Tubulinea are also groups of Amoebozoa; their defining characteristics include: Archamoebae lack mitochondria; Flabellinea flatten during locomotion and lack a shell and flagella; Tubulinea have a rough cylindrical form during locomotion with cylindrical pseudopodia. Free to share, print, make copies and changes. Get yours at www.boundless.com www.boundless.com/biology/textbooks/boundless-biology-textbook/protists-23/groups-of-protists-147/amoebozoa-and-opisthokonta-585-11802 Appendix Free to share, print, make copies and changes. Get yours at www.boundless.com Protists Key terms • conjugation the temporary fusion of organisms, especially as part of sexual reproduction • endosymbiotic that lives within a body or cells of another organism • hydrogenosome a membrane-bound organelle found in ciliates, trichomonads, and fungi which produces molecular hydrogen and ATP • kinetoplast a disk-shaped mass of circular DNA inside a large mitochondrion, found specifically in protozoa of the class Kinetoplastea • mitosome an organelle found within certain unicellular eukaryotes which lack mitochondria • osmoregulation the homeostatic regulation of osmotic pressure in the body in order to maintain a constant water content • plankton a generic term for all the organisms that float in the sea • plasmodium a mass of cytoplasm, containing many nuclei, created by the aggregation of amoeboid cells of slime molds during their vegetative phase • plastid any of various organelles found in the cells of plants and algae, often concerned with photosynthesis • pseudopodia temporary projections of eukaryotic cells • raphe a ridge or seam on an organ, bodily tissue, or other structure, especially at the join between two halves or sections • rhizaria a species-rich supergroup of mostly unicellular eukaryotes that for the most part are amoeboids with filose, reticulose, or microtubule-supported pseudopods Free to share, print, make copies and changes. Get yours at www.boundless.com Protists • saprobe an organism that lives off of dead or decaying organic material • sporangia an enclosure in which spores are formed (also called a fruiting body) • stipe the stem of a kelp • test the external calciferous shell of a foram Free to share, print, make copies and changes. Get yours at www.boundless.com Protists <em>Giardia lamblia </em> The mammalian intestinal parasite <em>Giardia lamblia,</em> visualized here using scanning electron microscopy, is a waterborne protist that causes severe diarrhea when ingested. Free to share, print, make copies and changes. Get yours at www.boundless.com Connexions. "Print ." CC BY 3.0 http://cnx.org/content/m44617/latest/Figure_23_03_02.jpg View on Boundless.com Protists Life cycle of <em>Trypanosoma brucei </em> <em>Trypanosoma brucei,</em> the causative agent of sleeping sickness, spends part of its life cycle in the tsetse fly and part in humans. Free to share, print, make copies and changes. Get yours at www.boundless.com Connexions. CC BY 3.0 http://cnx.org/content/m44617/latest/Figure_23_03_03.jpg View on Boundless.com Protists Dinoflagellates The dinoflagellates exhibit great diversity in shape.Many are encased in cellulose armor and have two flagella that fit in grooves between the plates.Movement of these two perpendicular flagella causes a spinning motion. Free to share, print, make copies and changes. Get yours at www.boundless.com Connexions. "Print ." CC BY 3.0 http://cnx.org/content/m44617/latest/Figure_23_03_04.jpg View on Boundless.com Protists <em>Paramecium</em>: sexual reproduction The complex process of sexual reproduction in <em>Paramecium</em> creates eight daughter cells from two original cells.Each cell has a macronucleus and a micronucleus.During sexual reproduction, the macronucleus dissolves and is replaced by a micronucleus. Free to share, print, make copies and changes. Get yours at www.boundless.com Connexions. CC BY 3.0 http://cnx.org/content/m44617/latest/Figure_23_03_07.png View on Boundless.com Protists Parasitic apicomplexans (a) Apicomplexans are parasitic protists.They have a characteristic apical complex that enables them to infect host cells.(b) Plasmodium, the causative agent of malaria, has a complex life cycle typical of apicomplexans. Free to share, print, make copies and changes. Get yours at www.boundless.com Connexions. "Print ." CC BY 3.0 http://cnx.org/content/m44617/latest/Figure_23_03_05ab.jpg View on Boundless.com Protists Bioluminescence Bioluminescence is emitted from dinoflagellates in a breaking wave, as seen from the New Jersey coast. Free to share, print, make copies and changes. Get yours at www.boundless.com Connexions. "Print ." CC BY 3.0 http://cnx.org/content/m44617/latest/Figure_23_03_05.jpg View on Boundless.com Protists Paramecium Paramecium has a primitive mouth (called an oral groove) to ingest food and an anal pore to excrete it. Contractile vacuoles allow the organism to excrete excess water.Cilia enable the organism to move. Free to share, print, make copies and changes. Get yours at www.boundless.com Connexions. "Basic CMYK ." CC BY 3.0 http://cnx.org/content/m44617/latest/Figure_23_03_06ab.jpg View on Boundless.com Protists Diatoms Assorted diatoms, visualized here using light microscopy, live among annual sea ice in McMurdo Sound, Antarctica.Diatoms range in size from 2 to 200 µm. Free to share, print, make copies and changes. Get yours at www.boundless.com Connexions. "Print ." CC BY 3.0 http://cnx.org/content/m44617/latest/Figure_23_03_09.jpg View on Boundless.com Protists Brown algae life cycle Several species of brown algae, such as the <em>Laminaria</em> shown here, have evolved life cycles in which both the haploid (gametophyte) and diploid (sporophyte) forms are multicellular.The gametophyte is different in structure from the sporophyte. Free to share, print, make copies and changes. Get yours at www.boundless.com Connexions. CC BY 3.0 http://cnx.org/content/m44617/latest/Figure_23_03_10.png View on Boundless.com Protists Oomycete A saprobic oomycete engulfs a dead insect. Free to share, print, make copies and changes. Get yours at www.boundless.com Connexions. "Print ." CC BY 3.0 http://cnx.org/content/m44617/latest/Figure_23_03_11.jpg View on Boundless.com Protists Stramenophile structure This stramenopile cell has a single hairy flagellum and a secondary smooth flagellum. Free to share, print, make copies and changes. Get yours at www.boundless.com Connexions. "CNX_Biology_Colors_Spot_2 ." CC BY 3.0 http://cnx.org/content/m44617/latest/Figure_23_03_08.jpg View on Boundless.com Protists Ammonia tepida Ammonia tepida, a Rhizaria species viewed here using phase contrast light microscopy, exhibits many threadlike pseudopodia. Free to share, print, make copies and changes. Get yours at www.boundless.com Connexions. "Print ." CC BY 3.0 http://cnx.org/content/m44617/latest/Figure_23_03_12.jpg View on Boundless.com Protists Forams These shells from foraminifera sank to the sea floor. Free to share, print, make copies and changes. Get yours at www.boundless.com Connexions. "Print ." CC BY 3.0 http://cnx.org/content/m44617/latest/Figure_23_03_13.jpg View on Boundless.com Protists Radiolarian shell This fossilized radiolarian shell was imaged using a scanning electron microscope. Free to share, print, make copies and changes. Get yours at www.boundless.com Connexions. "Print ." CC BY 3.0 http://cnx.org/content/m44617/latest/Figure_23_03_14.jpg View on Boundless.com Protists <em>Caulerpa taxifolia </em> <em>Caulerpa taxifolia</em> is a chlorophyte consisting of a single cell containing potentially thousands of nuclei. Free to share, print, make copies and changes. Get yours at www.boundless.com Connexions. "Print ." CC BY 3.0 http://cnx.org/content/m44617/latest/Figure_23_03_16.jpg View on Boundless.com Protists <em>Volvox aureus </em> <em>Volvox aureus</em> is a green alga in the supergroup Archaeplastida.This species exists as a colony, consisting of cells immersed in a gel-like matrix and intertwined with each other via hair-like cytoplasmic extensions. Free to share, print, make copies and changes. Get yours at www.boundless.com Connexions. "Print ." CC BY 3.0 http://cnx.org/content/m44617/latest/Figure_23_03_15.jpg View on Boundless.com Protists Plasmodial slime mold: Physarum polycephalum Physarum polycephalum is an example of a cellular slime mold. Free to share, print, make copies and changes. Get yours at www.boundless.com Wikipedia. "Fuligo92-300." CC BY http://en.wikipedia.org/wiki/File:Fuligo92-300.jpg View on Boundless.com Protists Badhamia utricularis Badhamia utricularis: an example of a plasmodial slime mold with the ability to form a fruiting body. Free to share, print, make copies and changes. Get yours at www.boundless.com Wikipedia. "Badhamia utricularis mature." CC BY http://en.wikipedia.org/wiki/File:Badhamia_utricularis_mature.jpg View on Boundless.com Protists Pseudopodia structures Amoebae with tubular and lobe-shaped pseudopodia, such as the ones seen under this microscope, would be morphologically classified as amoebozoans. Free to share, print, make copies and changes. Get yours at www.boundless.com Connexions. "Print ." CC BY 3.0 http://cnx.org/content/m44617/latest/Figure_23_03_17.jpg View on Boundless.com Protists A new protist is discovered that: has two long flagella which provide a means of locomotion; has a distinct groove to one side; and can obtain nutrients by either a heterotrophic or autotrophic method. This protist can be classified as: A) an euglenozoan B) a parabasalid C) a diplomonad D) a mitosome Free to share, print, make copies and changes. Get yours at www.boundless.com Protists A new protist is discovered that: has two long flagella which provide a means of locomotion; has a distinct groove to one side; and can obtain nutrients by either a heterotrophic or autotrophic method. This protist can be classified as: A) an euglenozoan B) a parabasalid C) a diplomonad D) a mitosome Free to share, print, make copies and changes. Get yours at www.boundless.com Boundless - LO. "Boundless." CC BY-SA 3.0 http://www.boundless.com/ Protists Which of the following is a characteristic of Dinoflagellates? A) encased by interlocking plates made of cellulose B) asymmetrically distributed vacuoles C) covered in rows of tiny cilia D) surrounded by a pellicle for protection Free to share, print, make copies and changes. Get yours at www.boundless.com Protists Which of the following is a characteristic of Dinoflagellates? A) encased by interlocking plates made of cellulose B) asymmetrically distributed vacuoles C) covered in rows of tiny cilia D) surrounded by a pellicle for protection Free to share, print, make copies and changes. Get yours at www.boundless.com Boundless - LO. "Boundless." CC BY-SA 3.0 http://www.boundless.com/ Protists All of the following are Stramenopiles EXCEPT: A) heterokonts. B) unikonts. C) diatoms. D) oomycetes. Free to share, print, make copies and changes. Get yours at www.boundless.com Protists All of the following are Stramenopiles EXCEPT: A) heterokonts. B) unikonts. C) diatoms. D) oomycetes. Free to share, print, make copies and changes. Get yours at www.boundless.com Boundless - LO. "Boundless." CC BY-SA 3.0 http://www.boundless.com/ Protists Which of the following characteristics is unique to Diatoms? A) silicon dioxide shells B) unicellular organisms C) photosynthetic activities D) live in colonies Free to share, print, make copies and changes. Get yours at www.boundless.com Protists Which of the following characteristics is unique to Diatoms? A) silicon dioxide shells B) unicellular organisms C) photosynthetic activities D) live in colonies Free to share, print, make copies and changes. Get yours at www.boundless.com OpenStax OER. "OpenStax College." CC BY 3.0 http://openstaxcollege.org/ Protists Which of these describes the largest difference between the shells of Foraminiferans and Radiolarians? A) Foram shells dissolve when the organism dies, while Radiolarian shells sink to the bottom. B) Foram shells have pointy spines,while Radiolarian shells are completely smooth. C) Foram shells exhibit radial symmetry, while Radiolarian shells exhibit bilateral symmetry. D) Foram shells are made of calcium carbonate, while Radilarians have shells made of silica. Free to share, print, make copies and changes. Get yours at www.boundless.com Protists Which of these describes the largest difference between the shells of Foraminiferans and Radiolarians? A) Foram shells dissolve when the organism dies, while Radiolarian shells sink to the bottom. B) Foram shells have pointy spines,while Radiolarian shells are completely smooth. C) Foram shells exhibit radial symmetry, while Radiolarian shells exhibit bilateral symmetry. D) Foram shells are made of calcium carbonate, while Radilarians have shells made of silica. Free to share, print, make copies and changes. Get yours at www.boundless.com Boundless - LO. "Boundless." CC BY-SA 3.0 http://www.boundless.com/ Protists Red algae differ from green algae in that the red algae: A) can be subdivided into chlorophytes and charophytes B) must contain phycoerythrins to be classifed as a red algae C) are primarily multicellular D) contain flagella which are used a sensor Free to share, print, make copies and changes. Get yours at www.boundless.com Protists Red algae differ from green algae in that the red algae: A) can be subdivided into chlorophytes and charophytes B) must contain phycoerythrins to be classifed as a red algae C) are primarily multicellular D) contain flagella which are used a sensor Free to share, print, make copies and changes. Get yours at www.boundless.com Boundless - LO. "Boundless." CC BY-SA 3.0 http://www.boundless.com/ Protists Which of the following traits are associated with the protists classified as slime molds? A) the pseudopodia associated with amoebozoa are hair-like B) the ability to produce spores that develop into a haploid zygote C) all these choices D) the ability to exist singly or as an aggregate of cells known as a slug Free to share, print, make copies and changes. Get yours at www.boundless.com Protists Which of the following traits are associated with the protists classified as slime molds? A) the pseudopodia associated with amoebozoa are hair-like B) the ability to produce spores that develop into a haploid zygote C) all these choices D) the ability to exist singly or as an aggregate of cells known as a slug Free to share, print, make copies and changes. Get yours at www.boundless.com Boundless - LO. "Boundless." CC BY-SA 3.0 http://www.boundless.com/ Protists Attribution • Connexions. "Groups of Protists." CC BY 3.0 http://cnx.org/content/m44617/latest/?collection=col11448/latest • Wiktionary. "kinetoplast." CC BY-SA 3.0 http://en.wiktionary.org/wiki/kinetoplast • Wiktionary. "hydrogenosome." CC BY-SA 3.0 http://en.wiktionary.org/wiki/hydrogenosome • Wiktionary. "mitosome." CC BY-SA 3.0 http://en.wiktionary.org/wiki/mitosome • Connexions. "Groups of Protists." CC BY 3.0 http://cnx.org/content/m44617/latest/?collection=col11448/latest • Wiktionary. "conjugation." CC BY-SA 3.0 http://en.wiktionary.org/wiki/conjugation • Wiktionary. "osmoregulation." CC BY-SA 3.0 http://en.wiktionary.org/wiki/osmoregulation • Wiktionary. "plastid." CC BY-SA 3.0 http://en.wiktionary.org/wiki/plastid • Connexions. "Groups of Protists." CC BY 3.0 http://cnx.org/content/m44617/latest/?collection=col11448/latest • Wiktionary. "stipe." CC BY-SA 3.0 http://en.wiktionary.org/wiki/stipe • Wiktionary. "saprobe." CC BY-SA 3.0 http://en.wiktionary.org/wiki/saprobe • Wiktionary. "raphe." CC BY-SA 3.0 http://en.wiktionary.org/wiki/raphe • Connexions. "Groups of Protists." CC BY 3.0 http://cnx.org/content/m44617/latest/?collection=col11448/latest • Wiktionary. "test." CC BY-SA 3.0 http://en.wiktionary.org/wiki/test • Wikipedia. "pseudopodia." CC BY-SA 3.0 http://en.wikipedia.org/wiki/pseudopodia • Connexions. "Groups of Protists." CC BY 3.0 http://cnx.org/content/m44617/latest/?collection=col11448/latest • Wiktionary. "plankton." CC BY-SA 3.0 http://en.wiktionary.org/wiki/plankton Free to share, print, make copies and changes. Get yours at www.boundless.com Protists • Wiktionary. "endosymbiotic." CC BY-SA 3.0 http://en.wiktionary.org/wiki/endosymbiotic • Connexions. "Groups of Protists." CC BY 3.0 http://cnx.org/content/m44617/latest/?collection=col11448/latest • Wikipedia. "Tubulinea." CC BY-SA 3.0 http://en.wikipedia.org/wiki/Tubulinea • Wikipedia. "Flabellinea." CC BY-SA 3.0 http://en.wikipedia.org/wiki/Flabellinea • Wikipedia. "Archamoebae." CC BY-SA 3.0 http://en.wikipedia.org/wiki/Archamoebae • Wikipedia. "Rhizaria." CC BY-SA 3.0 http://en.wikipedia.org/wiki/Rhizaria • Wikibooks. "Structural Biochemistry/Genome Analysis/Sequenced Genomes." CC BY-SA 3.0 http://en.wikibooks.org/wiki/Structural_Biochemistry/Genome_Analysis/Sequenced_Genomes#Amoebozoa • Wikipedia. "sporangia." CC BY-SA 3.0 http://en.wikipedia.org/wiki/sporangia • Wiktionary. "plasmodium." CC BY-SA 3.0 http://en.wiktionary.org/wiki/plasmodium • Wikipedia. "rhizaria." CC BY-SA 3.0 http://en.wikipedia.org/wiki/rhizaria • Wikibooks. "Structural Biochemistry/Three Domains of Life/Eukarya." CC BY-SA 3.0 http://en.wikibooks.org/wiki/Structural_Biochemistry/Three_Domains_of_Life/Eukarya • Connexions. "Groups of Protists." CC BY 3.0 http://cnx.org/content/m44617/latest/?collection=col11448/latest Free to share, print, make copies and changes. Get yours at www.boundless.com