RSF Animal Facility
Manu Sharma, PE
Facilities Management
Research Support Facility (RSF)
(AKA Building 30 or LAR)
•
•
•
•
KUMC’s largest animal building.
Original building with two floors built in 1987.
Two other floors added in 2003.
Both parts built with separate mechanical,
electrical and plumbing (MEP) systems fed
from the same sources. No interconnection
between the two.
• Lower level MEP systems are nearly 25 years
old and some are in need of replacement.
Understanding the HVAC for RSF
• HVAC- Heating Ventilation and Air Conditioning
• AHU- Air Handling Unit
Understanding the HVAC for RSF
• Air Terminal- Controls the room temperature by adding heat, meters the
air flow to help maintain room pressure
M E P System Study
• KUMC hired Engineering consultant Brack &
Assoc. in October 2011 to
– Review the condition of Mechanical, Electrical and
Plumbing (MEP) systems and assess their
reliability
– Recommend required replacements
– Recommend upgrades to have redundancy of MEP
systems, i.e. two separate water supply sources to
the building.
Study Findings
• Lower Level MEP systems are nearing the end of life and need
to be replaced, especially the air handling unit (AHU) serving
the lower levels (AHU-1)
• Lower level air terminals need to be replaced with new digital
controls for better performance and monitoring.
• Exhaust systems are not fully redundant.
• Lack of redundancy if steam or chilled water from power plant
is lost.
• Heating water and domestic hot water systems on lower level
need to be replaced and connected to upper level for
redundancy
Study Findings
(continued)
• Emergency power generators and electrical switchboards
need replacement
• Lack of redundant transformer
Our Plan Based on the Study Recommendations
•
•
Phased plan – funding limitations
Only Phase 1 and 2 have firm funding commitments, with some of the funds
for phase 2 coming from NIH grant (outcome not known)
• Phase 1 (Estimated Construction time Early June to December 2013)
- Install ductwork and controls to interconnect AHU-1, AHU-2 and
proposed AHU-3.
• Phase 2 (Estimated Construction time February 2014-November 2014)
– Install AHU-3
– Replace AHU-1
– Replace Air Terminals on Ground and First Floor with new controls
– Extend south stair to roof level
– Install a new water line for RSF, providing a redundant source of water.
Our Plan Based on the Study Recommendations
(continued)
• Phase 3
•
•
New Emergency Generator
New electrical switchgears for normal and emergency power
• Phase 4
•
•
•
•
•
•
•
New Boiler and Chiller for redundancy
New redundant heating system serving all parts of the bldg
New redundant domestic hot water system serving all parts of the bldg
New redundant exhaust fans for the whole bldg
New redundant transformer
New Edstrom system for Grd & first floors
New Elevators
• Phase 5
•
•
Build out penthouse for storage space
Build a barrier suite on second floor
AHU-1 Interior Pics
Time Line for Phase 1 Work
• Complete Design and Bid Documents for
interconnecting ductwork
– From Now - End of March 2013
• Bid the project
– Between April – End of May 2013
• Construction
– Between June – December 2013
Proposed Work on Ground Floor
Proposed Work on First Floor
Proposed Work on Roof
Impact of Construction in Phase 1
• Several Air supply shut downs to make modifications to
ductwork. Targeted to not last more than 4 hours at a time.
All shut downs shall be scheduled with RSF management.
• Will create temporary work spaces with partitions on
ground and first floor.
• Dust and construction traffic shall be controlled.
• Expect periodic noise and vibration in areas below, when
ducts and pipes are cut or attached to structure
Areas That May Experience Construction Noise/Vibration- Ground Floor
Areas That May Experience Construction Noise/Vibration- First Floor
Areas That May Experience Construction Noise/Vibration- Second Floor
Areas That May Experience Construction Noise/Vibration- Third Floor
IACUC Chair/Regulatory
Veterinarian Update
Who am I and What can I provide to
the Research Community?
Nathan C Culley, DVM, DACLAM
Functions
• Provide assistance to the Office of Animal Welfare on
IACUC policies/procedures and regulations.
• Chair the IACUC
• Pre-review protocols for submission to the IACUC.
– Assist new investigators on the review process and protocol
development.
• Communication/consultation with the IO on the Animal
Care and Use Program
– Program needs/improvements
– Compliance issues
• Work together with The AV, Executive Director of the LAR,
and the Research Community to assure a compliant Animal
Care and Use Program at KUMC.
Upcoming Changes to the Program
• Occupational Health
– Update categories for all animal users.
• In person annual screening vs. on-line questionnaire
– Risk assessment to determine needs/services per protocol
• Protocol Review Process Changes
– 3 tier review process-Pre-review, Vet review, IACUC review
• Goal is to have the protocol polished prior to IACUC review
– Drop Vet Pain and Distress Consult form-Vet review will satisfy
regulations and provide “consult”
– Hard dead-lines
• New policies/procedures based on the New Guide and improvement in
the Animal Care and Use Program.
Changes to the Animal Care and
Use Program
Scott Bury, Ph.D.
Director, Office of Animal Welfare
Upcoming Changes to Program
• Up coming AAALAC inspection
– June-July 2013
– Updated policies on OAW and LAR sites
– Draft Policies for Review
• Triennial Training
– Discontinued annual online refresher
– Every 3 years, must attend LAR Animal User Training Session (status
determined at time of de novo)
• New ACUP Forms
– Drafts Posted on OAW website
– March and April Training Sessions
– April 1st old forms will no longer be accepted (addendum and ACUP)
Upcoming Changes to Program
• LAR Advisory Committee
– Dr. Warren Nothnick, Scientific Director of LAR
– Reinstitute the LAR advisory committee
– Communicating research communities concerns and insights
with LAR, OAW, IACUC and Senior Administration
• Research Staff Training by PI
– New requirement to document protocol specific training by lab
– Records must be maintained by PI
• Call for topics for upcoming OAW seminars
Attending Veterinarian Services
Doug Brandt, DVM
Attending Veterinarian
Laboratory Animal Resources
Veterinary Services
• Responsible for the overall health and welfare
of all animals on KUMC campus
• Clinical and surgical care of animals
• Rodent Health monitoring
• Assist with Model Development
– Surgical Assistance
– Anesthetic Support
– Biotechnical Services
– Other veterinary procedures
Veterinary Services
•
•
•
•
•
Animal procedure training
Controlled Drug dispensing
Pharmaceutical dispensing
Protocol development
Pain and Distress consultation
Pathogen Outbreak Management in
Rodent Colonies
• Recommendations will be made by the
Veterinary Staff to help contain the outbreak
as well as eliminate the pathogen
– Depopulation
– Rederivation
– Colony wide treatments
– Extended quarantine without breeding
– Others
Pathogen Outbreak Management in
Rodent Colonies
• When an outbreak is confirmed the affected
rooms will be placed on quarantine status
until it is lifted by the Veterinary Staff
– Enhanced PPE
– Cage handling techniques
– Quarantine Per Diem rates
• Veterinary Staff will have final authority on
how the colony will be managed
Operational Updates
Vilma Zolynas, DVM, MBA, MHRM
Executive Director
Laboratory Animal Resources
TOPICS
1. Critical Animal Life Safety
2. Housing of Animals Exposed to Chemical Hazards
3. Rat Space Provisions and Cage Change Frequency
4. LAR Financial Data
5. Study Budget Estimate
CRITICAL Animal Life Safety
LAR Emergency Preparedness Plan
• If a disaster occurs within the animal facility, such as fire, excess heat, or
chemical spill, and the animal facility becomes a harmful environment,
then animals shall be moved to a safe location to the extent possible.
• Reasonable and valid attempts will be made to recover animals by the
best means possible. If personnel have limited time to move or otherwise
take care of animals, then the animals shall be helped in with the
following priority:
-Attempts will be made to save all the USDA regulated species
-Critical/irreplaceable to the study animals participating in grant-funded research
(bar code cage card marked with a red dot). PIs should mark only a few mice
breeding pairs per line that cannot be replaced
-Non-critical animals
• If animals cannot be successfully evacuated, they will be humanely
euthanized.
• Mark cages with red dots by April 1st.
Upcoming Changes
‘Chemical Hazards SOP’
• Chemicals that are toxic, known or suspect carcinogens,
reproductive hazards, teratogens, mutagens and antineoplastic
agents may be used in animal protocols. All chemicals being used
must be documented in an Institutional Animal Care and Use
Committee approved protocol.
• Two separate SOPs: PROG 28 is Biological Hazards and PROG 48 is
Chemical Hazards
• Two procedure rooms will be equipped with Class II Type B hoods
• Training will be provided to researchers on as needed basis.
LOCATIONS:
• RSF procedure room G033 (234 sq. ft.) with dedicated animal
room G040 (111 sq. ft.)
• RSF procedure room 3011 (145 sq. ft.) with dedicated animal
room 3016 (225 sq. ft.)
• No animal transfer charges
RATS: Primary Enclosures and Space Provisions
Species
Dimensions of
Enclosure
Space requirement based on animal
size
Maximum Number
Animals/Enclosure
Guiding
Document
Enclosure
Composition
Minimum height 7 inches
in2
20 x 10.5 = 210
8 inch height
BREEDING CAGE
11.5 x 12 = 138 in2
7 inch height
GREEN LINE
CAGE
Rats
Weight, g
<100
Up to 200
Up to 300
Up to 400
Up to 500
>500
Floor Area,
in2
17
23
29
40
60
70
14.25 x 8.25 = 118
in2
7.25 inch height
BLUE LINE CAGE
17 x 10 = 170 in2
7 inch height
METABOLIC
CAGE
3 or
M + F + Litter
Polysulfone,
automatic
watering,
open top,
cage
1 if over 500 g
2 if 400 – 500 g
3 if less than 400 g
or
1 F + Litter
Green
Tecniplast
IVC
Polysulfone
The Guide,
internal SOP
2 if up to 400 g
1 if over 400 g
Blue
Tecniplast
IVC
Polysulfone
2
Stainless
Steel,
wirebottom
suspended,
open top
Minimum height 7 inches
RAT HUSBANDRY SOP: All rat cages are being changed twice a week. If the cage is clean (singled housed,
newly split animals and weaned litters), LAR technician will not change such cage to avoid unnecessary
animal handling.
LAR Historical Financial Data
FY2009-FYTD December 2012
Actual
Revenue
Internal KUMC Department Generated - (Per
Diems, Recharges, & Charges for Services)
Actual
Actual
Actual
Projected
FYTD
December31,
2012
FY2013
FY2009
FY2010
FY2011
FY2012
$1,326,397
$1,397,221
$1,608,302
$1,732,838
$826,753
61,533120,214
$1,387,930
$1,517,435
60,940
$1,669,242
59,951
$1,792,789
74,457 105,000
$901,210
$1,681,753
1,047,752
1,182,092
External Customer Generated - (Per Diems,
Recharges, & Charges for Services)
Total LAR Generated Funds
Research Overhead Fund - Institutional
Departmental Indirect
853,056
966,432
State Appropriations
116,021
172,939
Grant-Shared Equipment
Total KU & State Funds
Total Revenue
Actual
24,8651,135
$993,942
$1,140,506
$2,381,872
$2,657,941
320,324
$1,368,076
$3,037,318
495,372
$1,677,464
$3,470,253
$1,576,753
473,908 947,473
245,931 489,658
$719,839
$1,621,049
$1,437,131
$3,118,884
•Revenue represent cash flow from Per diems - collection from services runs on average 60 days from when
service provided.
•LAR started FY2013 with 4 external customers. As of the end of December we are down to 3.
•Over the last year, LAR has self funded around 54% of operations and received no outside funding for capital
equipment replacement. Major maintenance repairs are addressed by KUMC facilities, but LAR must fund
any outside contractor or equipment purchases required.
•We continue to see a slow steady decrease in animal numbers.
LAR Historical Financial Data
FY2009-FYTD December 2012
Actual
FY2009
Percent Funds received from KU & State
Percent of Total Expenditures Funded by KU &
State (Including funded Deficit Funds)
Benefits
Contractual Services
Maintenance
Travel
Telephone & Networking
Supplies
Capital Outlay
Total Expenditures
Ending Available (Deficit Funds)
FY2010
41.73%
42.91%
48.81%
50.73%
Actual
Expenditure
Payroll & OT
Actual
Actual
Actual
FY2011
Actual
Actual
Projected
FY2012
FYTD
December31,
2012
FY2013
45.04%
48.34%
Actual
Actual
FY2011
$1,820,322
FYTD
December31,
FY2012
2012
$1,737,949
$842,822
FY2009
$1,498,905
FY2010
$1,650,451
375,366
509,724
573,013578,616
82,729
147,773
124,767
137,376
127,129
129,252151,853
5,852
19,379
23,306
15,341
527,017
542,219
64,442
63,982
$2,711,066
$3,079,925
($329,194.00) ($421,984.00)
44.41%
23,476
19,514
288,352320,972
Actual
46.08%
Projected
FY2013
$1,685,644
292,943 585,886
75,733
62,040 130,645
137,153 174,000
18,8875,149
23,894
12,824
21,180
26,000
173,313 329,525
31,308427,699
183,749 313,749
$3,010,003
$3,335,603 $1,709,993
$3,266,629
$27,314.76
$134,650.00 ($88,944.00) ($147,745.00)
Note: In FY2012-FY2013, LAR invested substantial funds in upgrading facilities and equipment.
Upgrading LAR Facilities and Equipment
FY2012-FY2013
Building
Equipment/System
RSF
Animal Transfer Stations*
4
$32,703.74
RSF
Racks for Non-Human Primates
5
$46,125
RSF
Individually Ventilated Racks with Automated Watering*
13
$456,809
RSF
Automated Watering Cages for Rats
360
$20,120.40
RSF
Edstrom Automated Watering System Replacement
1
$149,761.99
RSF
Watering System Installation
1
$115,305.54
RSF-Smith East
Storage Racks for Cage Wash Area
22
$34, 500.50
RSF
Class II Type B Biochemical Hoods
2
$22,500.00
RSF
Installation of Biochemical Hoods
1
$47,000.00
RSF
Sound Insulation Project (Large Animal Rooms, RSF 1st Floor)
1
~$50,000.00
Grand Total
Quantity
Amount
$ 974,586.60
* Two animal transfer stations and ten individually ventilated racks were purchased from non-LAR funds. Total: ~$368,000
LAR Historical Financial Data
FY2009-FYTD December 2012
Staff Information
Payroll FTE's-Budgeted
Payroll FTE's-Actual
Revenue - LAR Animal
Animal Census
Service & Supplies*
Total
FY2009
50.5
45.5
FY2010
50.5
47.5
FY2011
48.5
42.5
FY2012
44.5
39.5
Dec. 2013
42.5
38
$1,277,332
$110,598
$1,387,930
$1,460,853
$56,582
$1,517,435
$1,575,431
$93,811
$1,669,242
$1,572,971
$219,818
$1,792,789
$815,126
$86,084
$901,210
15,816**
15,819*
4,738
4,492
4,022
Projected
FY2013
42.5
38
$1,519,669
$162,084
$1,681,753
Animal Cages\Billing Unit Per Month
Mice - Starting in FY2011 mice billed by cage
Rats
Non-Human Primates
Other
1,219
757
539
432
538
103
62
53
45
40
54
11
12
14
14
5,342
4,983
4,614
Total
Animal Care Days
Mice - Starting in FY2011 mice billed by cage
Rats
Non-Human Primates
Other
Total
-
5,783,791
445,368
37,702
5,815,573
276,969
23,907
1,733,523
206,748
19,388
1,678,360
179,089
16,495
756,065
103,190
7,456
1,479,845
206,378
14,296
35,219
6,302,080
4,250
6,120,699
4,420
1,964,079
5,909
1,879,853
2,881
869,592
7,471
1,707,990
* Re-bill of drugs & supplies was 55% in FY 2012 and 60% as of FYTD December 2012. Veterinarians billed 3.6% of their time
during FY2012 and 3.4% during FYTD December 2012. Technicians billed 14.7% of their time during 2012 and 6.2% during FYTD
December 2012. Balance is from charges for specific service fees as outlined on LAR's rate schedule.
** Mice (not cages)
Cost & Rate Analysis for FY 2012: The calculated unit cost below is based on the 2000 edition of the Cost Analysis and Rate Setting
for Animal Research Facilities produced by the Cost Manual Revision Committee under the auspices of the Comparative Medicine area
of the National Center for Research Resources (NCRR), a component of National Institutes of Health (NIH).
Total Accumulated
Costs
Cost Center
Technical Activities
$
272,742.72
$
-
*External
Rate
Survey
Calculated
# of Units Unit Cost Actual Rate Variance
$
227,868.0
44,874.72
Animal Husbandry
$
1
Lamprey Fish
$
$
-
2
Gerbil per diem
$
2,852.34
2,334
$
1.2221
$
0.6500
0.5721
$
-
3
Goat per diem
$
16,156.26
848
$
19.0522
$
12.3900
6.6622
$
4
Hamster per diem/Outside patron
$
650.87
357
$
1.8232
$
1.7400
0.0832
$
5
Mice Autoclaved Box per diem
$
789,155.09
593,815
$
1.3290
$
0.8190
0.5100
$
6
Mice Bio-Containment Box per diem
$
39,056.26
23,401
$
1.6690
$
1.0300
0.6390
$
1.150
7
Mice Conventional Box per diem
$
1,023,820.15
851,540
$
1.2023
$
0.7100
0.4923
$
0.930
8
Mice Diabetic Box per diem
$
11,360.29
4,729
$
2.4023
$
1.3400
1.0623
$
-
9
Mice Germfree Box
$
1,448.10
4,670
$
0.3101
$
0.3101
$
-
10 Mice Outside Patron Box per diem
$
5,717.14
1,890
$
3.0249
$
1.2049
$
-
11 Mice Sentinel & Training
$
7,405.05
35,664
$
0.2076
$
0.2076
$
12 Mice Sterile Box per diem
$
232,505.64
158,470
$
1.4672
$
0.9200
0.5472
$
0.990
13 Pigtail Monkey per diem
$
23,997.90
1,451
$
16.5389
$
11.0700
5.4689
$
11.430
14 Rhesus per diem
$
103,604.68
6,337
$
16.3492
$
11.0700
5.2792
$
11.430
15 Squirrel Monkey per diem
$
122,241.94
8,631
$
14.1631
$
9.6200
4.5431
$
11.430
16 Rabbit per diem
$
574.07
104
$
5.5199
$
3.0600
2.4599
$
3.670
17 Rabbit per diem/Outside patron
$
8,301.36
775
$
10.7114
$
7.3500
3.3614
$
-
18 Rat Autoclave per diem
$
39,953.75
34,486
$
1.1585
$
0.6400
0.5185
$
-
19 Rat Bio-containment per diem
$
8,372.30
6,445
$
1.2990
$
0.7500
0.5490
$
20 Rat Conventional per diem
$
134,638.89
124,288
$
1.0833
$
0.5900
0.4933
$
21 Rat Diabetic per diem
$
1,126.35
605
$
1.8617
$
0.8000
1.0617
$
-
22 Rat per diem/Outside patron
$
13,129.02
5,974
$
2.1977
$
1.3800
0.8177
$
-
23 Rat Sentinel & Training
$
2,566.50
10,820
$
0.2372
$
0.2372
$
-
24 Pig per diem/Outside patron
$
1,041.04
21
$
49.5732
$
21.1732
$
-
25
$
-
-
$
-
$
-
-
$
-
26
$
-
-
$
-
$
-
-
$
-
Total
$
-
-
$
-
$
-
1.8200
-
28.4000
-
1.950
-
-
0.620
2,862,417.73
Note: Total accumulated costs exclude cost associated with regulatory compliance, purchase of fixed assets and unallowable costs
per OMB Circular A-21.
*Source: Per Diem Rate Survey included the following institutions:
The Ohio State University, University of Colorado Health Science Center, University of Iowa, University of Illinois-Chicago
University of Kansas Medical Center, University of Kentucky, University of Minnesota-Twin Cities, University of Tennessee-Memphis
University of Oklahoma Health Science Center, University of Nebraska Medical Center
Peer Group Per Diem Rate Survey
FY 2013
SPECIES ►
PER DIEM
TYPE►
Mean
Median
KUMC
2013 Ranking
Pig
Dog
Rabbit
Rat
Mouse/Sterile
Mouse ABSL2
Guinea Pig
NHP
Hamster
(per animal)
(per animal)
(per animal)
(per cage 2.66)
(per cage 5)
(per cage 5)
(per animal)
(per animal)
(per cage 3.8)
Colorado
$33.10
Iowa
$22.32
Kentucky
$21.42
Illinois-Chi
$19.70
$16.96
Iowa
$20.91
Kansas
$16.09
Colorado
$14.63
Oklahoma
$13.07
$12.61
Colorado
$5.18
Kentucky
$5.05
Oklahoma
$4.64
*Ohio State
$4.39
$3.67
Kentucky
$2.79
*Ohio State
$2.12
Iowa
$1.73
Minnesota
$1.70
$1.68
Tennessee
$1.31
Kentucky
$1.23
Minnesota
$1.10
Oklahoma
$1.09
$0.99
Tennessee
$1.57
*Ohio State
$1.40
Minnesota
$1.40
Oklahoma
$1.32
Kentucky
Kentucky
$2.36
*Ohio State
$2.12
Tennessee
$1.96
Iowa
$1.85
Kansas
Kentucky
$18.10
Minnesota
$13.10
*Ohio State
$12.06
Iowa
$11.66
Kansas
Kentucky
$3.76
*Ohio State
$2.74
Iowa
$2.62
$1.95
Minnesota
$1.70
Kansas
Minnesota
Iowa
Kansas
Kansas
$1.23
$1.82
$11.63
Illinois-Chi
$14.91
Minnesota
$14.00
Oklahoma
$13.58
*Ohio State
$12.39
Tennessee
$9.70
UNMC
$9.22
$12.38
Illinois-Chi
$11.13
*Ohio State
$10.76
Kentucky
$10.45
UNMC
$9.12
Tennessee
$7.67
$3.55
Kansas
$3.21
UNMC
$3.14
Minnesota
$2.84
Illinois-Chi
$2.77
Tennessee
$1.97
$1.64
Colorado
$1.60
UNMC
$1.38
Oklahoma
$1.31
Tennessee
$1.30
Illinois-Chi
$1.25
$0.97
*Ohio State
$0.95
Colorado
$0.94
UNMC
$0.93
Illinois-Chi
$0.80
Iowa
$0.59
$1.15
Kansas
$1.08
Illinois-Chi
$1.02
UNMC
$0.96
Colorado
$0.94
Iowa
$0.59
Oklahoma
$1.81
$1.80
Colorado
$1.75
Minnesota
$1.60
UNMC
$1.41
Illinois-Chi
$1.35
Illinois-Chi
$11.55
$11.43
UNMC
$9.12
Oklahoma
$8.09
Tennessee
$7.59
Colorado
No Rate
$1.48
UNMC
$1.46
Tennessee
$1.24
Oklahoma
$0.62
Colorado
No Rate
Kansas
No Rate
Participants:
The Ohio State University, University of Colorado Health Science Center, University of Iowa,
University of Kansas Medical Center, University of Kentucky, University of Minnesota-Twin Cities,
University of Tennessee-Memphis, University of Illinois-Chicago, University of Oklahoma Health
Science Center, University of Nebraska Medical Center
* The Ohio State University will adjust rates in October, 2012
Information Needed For
Study Budget Estimate
• LAR will not prepare a budget estimate if PI presents only study
design described in the ACUP.
PI should prepare detailed study flow chart/plan.
• Supplies
Grand Total
$ 41413.57
• Animals
Grand Total (add 20% $ 49696.29
• Labor
buffer)
• Housing
• Equipment
• Price list of PPE, drugs, and expendables is posted on LAR website
Note: For example, the budget estimate for study is about ~42,000.
This is the best approximation of the costs involved for LAR to
support the study. Studies usually do not go as planned and
contingencies should be built into study budget.
If investigator decides to change the original study design, the study budget
estimate should not be relied upon when submitting to funding agency.
Study
Information
Animal
quantity
Example Of Study Budget Estimate
Cohort 1 (12 weeks)
Cohort 2 (6 weeks)
Cohorts 1 and 2
15
15
30
Room #
Number of Rooms
Rabbit PerDiem (rate per rabbit per day)
3017 RSF
1
3.06
Animal Procurement and Disposal
Room and Per
Diem
Labor Rates
Tech rate per hour (regular)
44.00
Tech rate per hour (overtime; after 3:30 pm on 66.00
weekdays; any time weekends)
Veterinarian rate per hour
80.00
Per Diem
Rate Per Rabbit Price per 30
rabbits
Shipping (29 rabbits at one time)
90.00
Single Rabbit (animal, tattoo, shipping crate and 171.75
diet gel)
2610.00
5152.50
Shipping (Shipping single rabbit for pilot imaging) 530.00
530.00
Procurement (applies to 30 rabbit order)
Set-up per rabbit (1 time only)
Incineration per rabbit
Room fee (rate per month; 30 rabbits will fit into
a single room; study + acclimation time is >3 mo)
22.77
180.00
300.00
84.00
Rate Per Rabbit Price per 15
rabbit cohort
Per diem rate of 3.21(cohort 1 of 15 rabbits--> 1 292.11
week acclimation + 12 weeks on study = 91 days
4381.65
Per diem rate of 3.21(cohort 2 of 15 rabbits--> 1 157.29
week acclimation + 6 weeks on study = 91 days
2359.35
22.77
6.00
10.00
21.00
Labor
Rate Per Rabbit Price per 30
rabbits
Veterinarian Anesthesia Monitoring (7 hours for 112.00
5 rabbits = 1.4 hours per rabbit)
3360.00
Vet Tech Surgical Room Clean-Up (1.5 hours; 6
total clean-ups)
66.00
396.00
Vet Tech Surgical Room Set-Up (1.5 hours; 6
total set-ups)
66.00
396.00
Vet Tech Surgical Prep (7 hours for 5 rabbits =
1.4 hours per rabbit)
61.60
1848.00
Example Of Study Budget
Estimate (cont.)
Extra Post-Op Labor and Supplies
Description
Rate per Rabbit Rate per 30
rabbits
Buprenorphine (4 doses 0.1 mg/kg)
Baytril (5 doses 20 mg/kg)
3 cc syringe with needle (9 per rabbit)
Vet Tech Post-Op Drugs (9 injections; 4
buprenorphine and 5 Baytril at 3 minutes per
injection = 27 minutes per rabbit
54.80
1.05
1.44
19.80
1644.00
31.50
43.20
594.00
Drugs
Ketamine (100mg/ml) dose 50 mg/kg
Xylazine (100mg/ml) dose 10/mg/kg
Buprenorphine SR (1 dose at 0.1 mg/kg)
Bupivivcaine 0.75% dose 4mg/kg
Lidocaine 2% dose 3.5 mg/kg
Baytril 100 dose 20 mg/kg
Glycopyrolate (0.2 mg/mL) 0.01 mg/kg
Meloxicam (compounded 2.5 mg/mL; 50mL 139.95
1.04
0.17
6.0375
0.01
0.74
0.22
0.90
31.05
5.22
181.13
0.00
0.32
22.05
6.51
27.05
Example Of Study Budget Estimate (cont.)
Expendables and Fees
3cc syringe with needle (5)
1cc syringe with needle (2)
4x4 gauze (approx 15 for each rabbit - skin prep, surgical pack, catheter
placement)
0.80
0.46
0.75
24.00
13.80
22.50
Chucks (approx 3 for each rabbit - prep, surgical table, recovery)
54.00
1620.00
Light handle covers (2 per table with 2 tables; 4 per day; 24 used over 6
surgery days)
2.17
65.04
IV catheter
IV set
500ml Saline
Warming blanket (BAIR HUGGER - one may last for every day of 5 rabbits;
need 6 blankets to cover 6 surgery days)
1.40
1.33
1.43
5.58
42.00
39.90
42.90
167.40
Suture scaffold (4-O PDS Maxon 231.25 per box; 1 pack per rabbit; 1 box
should cover 30 rabbits)
7.71
231.25
Suture (muscle, SQ, skin - 2 pack of 2-0 vicryl per rabbit)
14.54
436.20
Bouffont cap (5 personnel; surgeon, assistant surgeon, anesthetist,
preptech, post-op monitor)
0.04
1.20
Tie-on facemask (5 personnel)
0.15
Non-sterile gloves (3 personnel; anesthetist, preptech, post-op monitor; 10 1.68
changes per staff member per day = 30 pairs)
4.50
50.40
Sterile surgical gown (2 personnel; 1 change per day = 4 gown per surgery
day
Sterile gloves (2 personnel; 2 pairs per surgery X 5 surgeries = 10 pairs + 2
extra for = 12 pairs per 5 rabbits = 2.4 pairs per rabbit.
Shoe covers (5 personnel; 1 change per day = 10 pairs per day)
9.46
283.92
2.42
72.72
0.56
16.80
Thermovent hepa filter (1 per animal)
Suture surgical pack (1 per animals)
Mayo stand cover
Isoflurane (7 hours for 5 rabbits = 1.4 hours per rabbit)
3.52
11.00
3.06
38.56
105.60
330.00
91.80
1156.68
OR equipment and room fee
Needles (misc use; drug draws, fluid draws, etc) 5 per rabbit
100.00
0.50
600.00
15.00
Example Of Study Budget Estimate (cont.)
Bronchoscopy and IVIS
Description
Rate per rabbit (single Rate for 90 scans Notes
bronchoscopy and IVIS (15 rabbits scoped
scan)
and scanned on
wks 3, 6, 9, 12; 15
rabbits scoped and
scanned on weeks
3 and 6)
IVIS pilot study (approx 2 hours; include isoflurane, vet tech time, 436.29
durgs, IVIS room fee, IVIS technician, etc) Single rabbit
436.29
Ketamine (100mg/ml) dose 50 mg/kg
Xylazine (100mg/ml) dose 10/mg/kg
3 cc syringe with needle (2 per rabbit scope and scan for
ketamine/xylazine sedation)
1 cc syringe with needle (1 per rabbit scope and scan for
meloxicam)
Vet Tech Time IVIS scan week 0 (10 rabbits in 1 day - approx 40
minutes per rabbit including sedation, placement in IVIS scanner,
IVIS scanner, recovery) includes overtime = ~6.67 hours
1.04
0.17
0.32
93.15
15.66
28.80
0.23
20.70
29.48
884.40
Vet Tech time Bronchoscopy and IVIS scan weeks 3,6,9,12 (5
36.61
rabbits in 1 day - approx 50 minutes per rabbit including sedation,
bronchoscopy, placement in IVIS scanner, IVIS scanning, recovery)
includes overtime = ~4.16 hours for 5 rabbits in one day
3294.72
Room rate (85.00/hr) ~4.16 hours for 5 rabbits in one day
6364.80
70.72
IVIS technician 61.00/hr (required for first few sessions; may not 50.75
be needed for later sessions if PI/research lab gets training from
LAR and IVIS technician)
1 rabbit only
30 scans (10 rabbit
done on 3 separate
days)
761.28
Assuming 3
sessions (15 scans
prior) Lab may be
trained and
operate scanner
after this period
LAR Budget Estimate Agreement for Biotechnical Services
I __________________________________(PI name) have read the budget estimate provided by LAR for the animal
study slated to begin approximately (date) __________________ and end approximately
(date)________________under ACUP#_____________. I understand that this is the best approximation of the costs
needed for LAR to support my study. While the fixed prices for labor (technical service rates) and supplies (supply price
list) and animal housing (per diem rate) have been provided, I also understand that the costs may differ (actual cost
may be higher or lower than initial estimate) based on and not limited to the actual time needed to complete various
services provided by LAR and the actual number of supplies needed for the procedures. Additionally, if animals suffer
post-operative complications, there will be additional costs associated with the labor and supplies needed to perform
treatments and observation to address those complications. If on-going or intense technical support is needed, it may
be more cost-effective to hire a dedicated technical support person for my laboratory. I have also discussed details
related to animal caging needed for this study, and I am satisfied with the caging supplied by LAR for this particular
study.
•
•
•
•
Provisions of LAR Biotechnical Services:
Biotechnical services are provided by LAR on an ‘as available’ basis without warranties of any kind, either expressed or
implied (For example, if an LAR employee, who provides technical support for the study, gets sick or resigns, there may
be resulting delays for the study. However, LAR and the lab may work together to procure replacements for such
staffing positions).
LAR will not issue credits for lab animals that are not suitable for study. LAR will make every effort to ensure animals
are healthy prior to shipment into LAR facilities. Furthermore, if animals are deemed unhealthy or unsuitable upon
arrival, with input from the lab, LAR may seek animal replacements from the animal vendor. However, no such
replacements are guaranteed
Also, LAR will not issue credits for services (i.e. per diem accrual) due to decisions made by the lab. (For example, if a
lab chooses to delay procedure in order to work with a specific LAR staff member when an adequate alternative staff
member is available, LAR will not be responsible for the additional per diems associated with the study delay).
If the PI is dissatisfied with LAR technical service or any terms, guidelines, or practices, the sole and exclusive remedy is
to discontinue using the services. If the investigator decides to change the original study design, this budget estimate is
automatically invalid and should not be used for submission to funding agency.
_______________________________
____________________
Signature of PI/Investigator
Date