Introduction to Obstetrics and Gynecology Anatomy and Physiology Assoc. Prof. Gazi YILDIRIM, M.D. Objectives • To learn – female reproductive anatomy – Pelvic anatomy – Pelvic innervation – Pelvic blood supply – Basic hormon knowledge – Steroid hormon pathways – Female reproductive endocrinology Abdominal Wall • • Skin Subcutaneous fat (superficial fascia), which below the navel can be considered as two layers: – Camper’s fascia; the superficial layer containing fat, – Scarpa’s fascia; fibroelastic membrane. • Muscles, The abdominal muscular wall can be considered a group of 4 paired muscles along with their aponeurosis: – The three lateral muscles (from superficial to deep) are the • External abdominal oblique • Internal abdominal oblique • Transversus abdominis muscle – Medially muscles are the: • Rectus Abdominis • Pyramidalis muscles • • Preperitoneal fat Peritoneum – Parietal peritoneum peritoneum of anterior abdominal wall • Visceral peritoneum peritoneum investing the viscera Anatomy: Perineum • • • • • • • Clitoris Urethra Vagina Anus Labia majora and minora Bartholin’s and Skene’s glands Hymen Elements comprising the Pelvis • Bones – Ilium, ischium and pubis fusion • Ligaments • Muscles – Obturator internis muscle – Arcus tendineus levator ani or white line – Levator ani muscles – Urethral and anal sphincter muscles Bony pelvis • Composition: formed by paired hip bones, sacrum, coccyx, and their articulations • Two portions – Greater pelvis – Lesser pelvis • Terminal line ( pelvic inlet): formed by promontory of sacrum, arcuate line, pectin of pubis, pubic tubercle, upper border of pubic symphysis • Pelvic outlet: formed by tip of coccyx, sacrotuberous ligament, ischial tuberosity, ramus of ischium, inferior ramus of pubic symphysis Anatomy: Bones of Pelvis • Sacrum • Coccyx • Innominates (2) • Ilium • Ischium • Pubis • Anatomical antero-posterior diameter (true conjugate) = 11cm • Obstetric conjugate = 10.5 cm (-2cm from diagonal conj) • Diagonal conjugate = 12.5 cm Pelvic Floor Muscles Levator Ani •Puborectalis •Pubococcygeus •Ileococcygeus Obturator Internus Piriformis Coccygeus Levator Plate Netter F Atlas of Human Anatomy Novartis 1997 “Ligaments and Fascia” Cardinal/Uterosacral Complex (Delancy level I) Paracervical Ring Arcus Tendineus Fascia Pelvis ATFP Pubocervical “fascia” Rectovaginal “fascia” Perineal Body Perineal Membrane Superficial Pyramidal Internal External Transverse Anal Fibrous AnalSphincter Sphincter perineus Body sphincter) Bulbocavernosis (urethrovaginal Cleveland Clinic Foundation Burnett Novack’s Gynecology 2004 • Sphincter urethrae externus • Bulbospongiosus • Ischiocavernosus • Transversus perinei profundus • Transversus perinei superficialis Bony structures of a pelvis M. Urethra ischiocavernosus M. bulbospongiosus M. transversus perinei superficialis Perineal muscles M. sphincter ani externus Vagina Urethra Vagina M. transversus perinei profundus Urogenital fascia M. transversus perinei superficialis M. sphincter ani externus ATFP Pelvic floor Add rectum Rectal connections Vaginal connections Urethra Bladder Cervix lig.cardinale Lig. sacrouterina Lig. cardinale Layers of the Anterior Triangle of the Perineum • Skin • Subcutaneous tissue .Camper's fascia .Colles fascia • Superficial space .Clitoris and its crura .Ischiocavernous muscle .Vestibular bulb .Bulbocavernous muscle .Greater vestibular gland .Superficial transverse perineal muscle • Deep space-perineal membrane .Compressor urethrae .Urethrovaginal sphincter • Ürogenital trigon: – – – – M. Transversus perinei superficialis M. İschiocavernosus M. Bulbocavernosus M. Transversus perinei profundus • Anal trigon: – M. Levator ani – M. Sphincter ani externus FIGURE 7.2. Superficial compartment and perineal membrane. EKSTERNAL ACCESSORY ORGANS (Vulva – Pudendum) • • • • • • Mons pubis Labium majus Labium minus Clitoris Bulbus vestibuli Gl. vestibularis major • Gl. vestibulares minor Labium Majus Pudendi • • • • • • Correspond to the scrotum Includes numerous hairs Rima pudendi Commisura labiorum ant. Commisura labiorum post. Lig.rotundum Rima pudendi Commissura post Labium Minus Pudendi (Nymphae) • • • • • • • Includes sweat glands Preputium clitoridis Frenilum clitoridis Sulcus nympholabialis Frenulum labiorum pudendi Fossa navicularis Fourchette Sulcus nympholabialis Frenulum labiorum pudendi Frenulum clitoridis Clitoris • Corresponds to the male penis • Corpus, crus, glans • Attach to the side of the pubic arch with Lig. Suspansorium Vestibüle - Introitus (Vestibulum Vaginae) • The Vaginae and ostium urethra externa opens vestibüle • Covered by stratified squamous epithelium Vestibül Glandula Vestibularis Major (Bartholin’s glands) • Correspond to the male bulbourethral glands • Covered by M.bulbocavernosus • Secrete fluid that moistens and lubricates vestibule Glandula Vestibularis Minor (Skene glands) • Opening either side of the urethra • To get Gonore retansiyon abscess Skene kanalları FIGURE 7.2. Superficial compartment and perineal membrane. Hymen Feminus • Mucosal fold • Carunculae myrtiformis Anatomy: Pelvic Innervation • Pelvic splanchnic nerves from 2nd to 4th sacral nerves • Pudendal nerve supplies vulva and lower vagina • Vulva blood supply comes from a terminal branch of the a.iliaca interna a.pudenda interna • N. pudendus INTERNAL ACCESSORY ORGANS • • • • Vagina Uterus Uterine tubes Overies Vagina (Kolpos) • • • • • • • • Fibromuscular tube about 9 cm Fornix vaginae Columna rugarum Tunica mucosa Tunica muscularis Tunica adventisya A. vaginalis V. iliaca interna Vajina Uterus (Metra - Hystera) • 8 x 6 x 4 cm 40-50 g. • Corpus, isthmus, collum (cervix) • Portio vaginalis uteri • • • • • Serosal layer (perimetrium) Muscular layer (myometrium) Mucosal layer (endometrium) Excavatio vesicouterina Excavatio rectouterina (Douglas ) Squamocolumnar Junction Tuba Uterina (Fallop Tüpleri-Salpinx) • İnfundibulum (fimbria, fimbriae ovarica) pars ampullaris, isthmica, interstitialis • A.ovarica ve A.uterina Ovaries • • • • Medulla (blood and lenf vessels, nerves) Cortex (germinal epitel, tunica albuginea, hilum ovarii) Lig. Ovarii Proprium (between overies and uterus) Lig. Suspensorium Ovarii (infundibulopelvic) (between overies and pelvic side wall) • A.ovarica, plexus pampiniformis Pelvic Vasculature Common Iliac Sakralis Media İnternal iliak (hipogastrik) İliolumbar Lateral Sakral Superior Gluteal Inferior Gluteal Orta Hemoroidal Pudendal Uterin Inferior Vesika Umbilical Obturator Burnett Novack’s Gynecology 2004 Branches of the Internal Iliac Artery Anterior Division Posterior Division Uterine Umbilical Uterine vesical Obturator Internal pudendal Inferior gluteal Middle vesical Middle rectal Vaginal Superior gluteal Lateral sacral Iliolumbar Collateral Arterial Circulation of the Pelvis Primary Artery Collateral Arteries Aorta Ovarian artery Uterine artery Superior rectal artery (inferior mesenteric artery) Middle rectal artery Lumbar arteries Vertebral arteries Inferior rectal artery (internal pudendal) Iliolumbar artery Iliolumbar artery Middle sacral artery Lateral sacral artery External Iliac Deep iliac circumflex artery Inferior epigastric artery Femoral Medial femoral circumflex artery Lateral femoral circumflex artery Iliolumbar artery Superior gluteal artery Obturator artery Obturator artery Inferior gluteal artery Superior gluteal artery Iliolumbar artery • Ovarian arteries – Originate directly from the aorta, inferior to the renal arteries. – Most frequently identified at the IP ligament. • Ovarian veins: – Left ovarian vein drains into the left renal vein – Right ovarian vein drains directly into the inferior vena cava. Ligaments of the Uterus • Lig.Sacrouterinum (LUNA) • Lig.Latum Uteri • Lig.Rotundum – Cyst of Nuck canal – A.Sampson – Lig. Cardinale most important lig. 8 Avascular Spaces • • • • • • Prevesical (1) Vesicouterine (1) Rectovaginal (1) Presacral (1) Paravesical (2) Pararectal (2) Lymph Drainage • The external genitalia, anus, and anal canal drain to the superficial inguinal nodes • The lower one third of the vagina drains to the sacral nodes and the internal and common iliac nodes • The cervix drains to the external or internal iliac and sacral nodes Lymph Drainage • The lower uterus drains to the external iliac nodes • The upper uterus drains into the ovarian lymphatics to the lumbar nodes. The lymphatics of the ovaries drain out of the pelvis to the lumbar nodes Lymph Drainage • Cervical Cancer: – Drains 1st to the parametrial nodes --> obturator nodes --> pelvic nodes --> para-aortic • Uterine Cancer: – Drains 1st to the pelvic nodes or para-aortic. • Ovarian Cancer: – Can metastasize to either the pelvic or para-aortic nodes. Pelvic Autonomics • Superior hypogastric plexus (presacral nerve) – Contains no parasympathetics • Hypogastric nerve • Inferior hypogastric plexus – Contains parasympathetic fibers from the pelvic splanchnics • Ganglion impar Pelvik Organların Sinirleri Pelvik Organların Sinirleri Pelvic Vasculature Common Iliac Sakralis Media İnternal iliak (hipogastrik) İliolumbar Lateral Sakral Superior Gluteal Inferior Gluteal Orta Hemoroidal Pudendal Uterin Inferior Vesika Umbilical Obturator Burnett Novack’s Gynecology 2004 Pelvic Innervation Lumbar Pleksus Sacral Pleksus Genitofemoral n. Obturator n. Femoral n. Pudendal n. Obturator Foramen Sciatic n. Hipogastrik Plexus (Otonomic) Pelvik Plexus (Otonomic) Irvin W Obstetrics/Gynecology 2003 Folliculogenesis and Normal Physiology Follicular Maturation FSH • Induces early growth • Controls follicle number LH • Provides estrogen precursors • Needed for latter stages of growth Erken Foliküler Faz Thecal cell LH Androstenedione Testosterone Granulosa cell FSH IGF II Aromatisation E1 E2 Asetat Kolesterol Sitokrom P450 scc Progesteron Pregnanolon 17 OH Pregnanolon 17-20 Desmolase 17 α OH ase 3 β OH SDH 17 α OH ase 17 OH Progesteron 17-20 Desmolase Androstenedion DHEA 17 β OH SDH Androstenediol E3 17 β OH SDH Testosteron 5 α redüktase DHT ? E1 17 β OH SDH E2 Gonadotropin Releasing Hormone (GnRH) • • • • Secreted by hypothalamus Pulsatile secretion is critical for activity GnRH secretion inhibited by estrogen Low levels of estrogen cause hypothalamus to release GnRH Follicle-stimulating Hormone (FSH ) • • • • FSH hormone is secreted by anterior pituitary Turned on by GnRH Negative feedback inhibition with estrogen Causes maturation of dominant ovarian follicle Luteinizing Hormone (LH) • • • • Secreted by anterior pituitary Turned on by estrogen Maintains corpus luteum Causes corpus luteum to secrete progesterone Positive feed back of E2 more than 200 pg /ml > 50 hours A threshold of LH 14-27hours for full maturation of the oocyte to occur The End