The Peritoneal Vac Study
Instructions for the ICU Nurse
PI: Andrew Kirkpatrick pg # 02603
Derek Roberts pg# 02871
Corina Tiruta pg # 00521
For each patient enrolled in the study, blood and peritoneal
fluid will be collected in the ICU four 4 times. Use the kits
stored in the lab space in the box labeled “Peritoneal Vac
Study”.
•
Collection 1: - 24 hrs after the temporary abdominal dressing
(TAC) is initiated (24 hrs after the OR procedure ends)
-use the kit labelled with pt.id.001-2 for patient
one, pt.id.002-2 for patient two, etc.
•
Collection 2: - 48 hours after TAC (48 hrs after the OR
procedure ends)
-use the kit labelled with pt.id.001-3 for patient
one, pt.id.002-3 for patient two,etc.
•
Collection 3: - day 6 after TAC (144 hours after the OR
procedure ends)
-use the kit labelled with pt.id.001-4 for patient
one, pt.id.002-4 for patient two
•
Collection 4: - day 26 after TAC or discharge, whichever
comes first
-use the kit labelled with pt.id.001-5 for patient
one, pt.id.002-5 for patient two
e.g. OR procedure ends at 2 am on Jan.1 -> Collection 1 will be done at 2 am on Jan.2, Collection 2 at 2 am on
Jan.3, Collection 3 at 2 am on Jan. 7, Collection 4 at 2 am on Jan.28 (or hospital discharge)
FOR EACH TIME POINT COLLECTION:
-
1. COLLECT PERITONEAL FLUID:
fill-up the 4ml green cap tube labelled “PF” with peritoneal
fluid.
2. COLLECT BLOOD:
-
2 Green Cap 4ml tubes for plasma isolation (labeled with a
“P”)
2 Red Cap 4ml tubes for serum isolation (labeled with a “S”)
fill-up the tubes to 4ml and keep them on the rack provided
in an upright position
3. Fill-out the pt. gender & DOB, collection date&time on the
Research Requisition
4. Send the five (4ml) tubes, the rest of the bag (containing 3
empty blue cap 15ml tubes for CLS use only) and the
Research Requisition to CLS by porter
STEP 1: Peritoneal Fluid Collection:
PF
Fill with Peritoneal Fluid
STEP 2: Blood Collection:
S
S
P
P
Fill with Blood from arterial line
and keep upright in the rack
STEP 3: Fill the requisition
Fill-out the patient’s gender & D.O.B. and collection date and time on
the research requisition (RR)
STEP 4: Calgary Lab Services:
Call a porter to have the five 4mls tubes + the RR and the
rest of the bag deliver to CLS