For On-Call Attending Surgeons

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The Peritoneal Vac Study
How do I enrol a patient
in this study?
PI: Andrew Kirkpatrick
RC: Corina Tiruta
1. Decide if an open abdomen is the indicated therapy
for your patient. If so, continue to step 2; If not, do
not enrol this patient into this study.
Exclude: patients under 18 years old, pregnancies, patients undergoing
intraperitoneal chemotherapy
1. Go to www.peritonealvac.com
2. Select “Randomization” hot-key (top right corner)
3. Enter the super-simple Username and Password (if
forget can page Andy #02603, 24/7)
4. Enter patient RHRN + last name to randomize
5. You’ll be indicated the patient number you have
randomized. Use the corresponding bag (located in
the AbThera dressing tote, in the Peritoneal Vac
Study box) for blood & peritoneal fluid collection.
e.g.: for randomized pt.1 use the kit labelled with pt.id 001-1
7. Collect PERITONEAL FLUID:
- If Mon. to Fri., 8am to 4pm: fill-up one 50ml blue
cap tube and one 4ml green cap tube labelled “PF”
with peritoneal fluid, and page Caroline Leger at
07559 to collect the 50 ml tube.
- If after hours: fill-up one 4ml green cap tube
labelled “PF” with peritoneal fluid; discard the 50ml
tube.
8. Collect BLOOD If Mon. to Fri.8am to 4pm or after
hours:
- 2 Green Cap (4ml) tubes for plasma isolation
(labeled with a “P”)
- 2 Red Cap (4ml) tubes for serum isolation (labeled
with a “S”)
fill up the tubes to 4ml and keep them on the rack provided in an upright
position
* blood will be drawn by anaesthesia team
9. Fill-out the pt. gender & DOB, collection date&time
on the Research Requisition
10. Send the five (4ml) tubes, the rest of the bag
(containing 3 empty blue cap 15ml tubes for CLS
use only) and the Research Requisition to CLS by
porter
11. Place the type of Temporary Abdominal Dressing
indicated by randomization
- for further information on actually dressing placement
refer to Regional Trauma Services Guidelines for the
Mgmt.of the Open Abdomen (internal website under Trauma
Services/Adult Trauma Care/Policies,Protocols and Practice Guidelines)
or page Andy #02603 24/7
Monday to Friday
8am to 4pm:
STEP 2: Blood Collection:
STEP 1: Peritoneal Fluid
Collection:
Don’t send the
50ml tube to CLS.
page Caroline at
07559 to pick it up
PF
PF
Fill BOTH with
Peritoneal
Fluid
Fill with Blood from arterial line or
venipuncture and keep upright in
the rack
STEP 3: Fill the requisition
STEP 4: Calgary Lab Services:
Call a porter to have the 5 tubes (green and red cap) +
the research requisition and the study bag containing 3
unfilled 15ml transfer tubes delivered to CLS
Monday to Friday after 4pm,
week-ends and holidays
STEP 2: Blood Collection:
Fill with
Peritoneal
Fluid
Fill with Blood from arterial line or
venupuncture and keep upright in the rack
PF
STEP 1: Peritoneal Fluid
Collection:
STEP 3: Fill the requisition
STEP 4: Calgary Lab Services:
Call a porter to have the 5 tubes + the research
requisition and the study bag containing 3 unfilled
transfer tubes delivered to CLS
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