Title: Identifying Nutrients: Carbohydrates, Proteins, and Lipids ► Abstract: There are six essential nutrient groups that make up 96% of all living things. Carbohydrates, Proteins and Lipids are three of these categories that can be identified in food sources by testing for the presence with reagents such as Lugols, Benedicts, Biurets and Sudan III. These chemicals changed colors to indicate the positive presence of one of these nutrient groups. Introduction ► Background Information: Use your lab handout . The handout provided important information on the role of proteins, carbohydrates and fats; the structure and function of each group and what food sources usually have these food groups. Objective ► The objective of this study was to determine the presence of ______________, ________________, and___________________. If certain chemicals called indicators are added to food groups, then a color change will occur and identify if one of the nutrients is present. Methods ► The method to performed the experiment is similar to one documented by (add internet URL) ____________________________________ ______________________________. Four test tubes were filled with a small quantity of one food item each: ____________________________________ ___________________________________. ► Also, potatoes and lard were placed in individual weigh boats and were also tested with the same indicators. The weigh boats were used to avoid clogging test tubes. The test tubes were filled with 3 droppers of one particular reagent. ► The four tests were run using the following reagents: Lugols, Benedicts, Biuret and Sudan III. Between each trial, test tubes were ____________ and ____________, to avoid contamination. Furthermore, when testing with Benedicts, the test tubes were placed in a water bath to break down the sugar bonds. Results: ► The following chart denotes the food items and the reagent. A color change indicates the presence of Protein, Carbohydrates and Fat. ► Insert table, chart or graph. Discussion ► The author noted that the potato responded to the reagent ____________ by changing from yellow to ________________. This color change tests positive for ____________. Therefore, potatoes must contain ________________. ► Continue with the rest. ► (Make sure you include errors, repeats, weird observations and try to explain them…did you forget to place test tubes in the water bath? Did you heat a test tube with the wrong reagent? Etc.) Conclusion ► The nutrient groups Proteins, Carbohydrates and Fats are essential life. A source of protein is found in __________. Biurets will change from ________to _________ indicating proteins. Heat will speed up the reaction time. ► Continue with your conclusions… Reference and Acknowledgement ► List ► End the Internet URls, with a quick few sentences acknowledgement that your lab partner (s) provided. What test did they perform?, did they assist?, lead, not do anything etc.