RECRUITMENT TALK
Remember
There are few positions and many candidates
200-500 candidates for any tenure track
faculty
position
10 qualified people for each position
I receive 30-50 unsolicited requests per month
Step 1: Getting the Interview
CV and personal statement
Review standard University CV formats
I do not care what you think your skills are
I want to know what you have produced
I want to know what your training is
I will respond better to what I am used to
seeing
RECRUITMENT TALK
• What am I looking for in a trainee
candidate
• What am I looking for in a faculty
candidate
RECRUITMENT TALK
• You only get one chance to make a first
impression
– Arrive early
– Dress Professionally
• Males Tie and Jacket
• Females Consider a suit
– At least a dress or skirt.
• Live with your clothes. Do not fidget
• I am looking for a professional colleague
– Slides must be clear, single format
RECRUITMENT TALK
• What am I looking for in a trainee candidate
– Career goals
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What do you want to do in 5 years
How will you get there:
What are your strengths and weaknesses
Are you driven to succeed
– Talk
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Organization
Thought process
Much more important than the data
Handling questions more important than the talk
– NEVER EVER INTERRUPT
– I am making a commitment to your career
• Will you be successful
• Are you teachable
• Do you want to learn or do you already know it all
RECRUITMENT TALK
• What am I looking for in a Faculty Candidate
– Someone I trust
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Represent me in meetings
Not cause problems in and for the department
A team player and collaborator: Team sports
There is no “I” in a talk
– Someone I want to work with, go to dinner with, go
to meetings with
– Someone who will make me better or my life easier
• Will they teach classes well
– Will they get grants
– Are they going to be a good teacher
– Are they intellectually curious; Will they listen to my
questions. Will they review my grant and help me
– Have they asked their supervisor about the project
and what they can take with them
RECRUITMENT TALK
– There are many candidates.
– Recruitment is occasional.
– For students, we are looking at being
• together for 4-6 years, PDFs 3-4 years, faculty a
lifetime.
– Divorce (termination) is painful
– Estimated cost of a “wrong” faculty
rectruitment: 750K
– There are many ways to make a mistake, few
to make a good first impression
– Better to recruit no one than the wrong one
RECRUITMENT TALK
3 PARTS
Scientific Presentation
One on one interviews
Dinner
RECRUITMENT TALK
PREPARATION FOR THE TRIP
Its not all about the talk
DO YOUR HOMEWORK.
Institution
If you are going to Duke, know about the basketball team
If you are going to UT, know about the football team
Texas A and M, know what an Aggie is.
People
Study your agenda
Know who you are meeting.
Review online.
What is their latest paper. Know their history
Many people have websites or lab websites
IL2, LAKs and TILs
Know who you are going to dinner with
RECRUITMENT TALK
TALK
Prepare practice and give it to as many people
who will listen
Have people look at slides
Spelling errors, too busy
Use SpellCheck
PDF presentation is safest approach
Do not tell jokes or try to be funny.
You can have an amusing slide
NO motion slides unless absolutely necessary
If possible overlay slides if motion needed
RECRUITMENT TALK
THE TALK
Expect to be nervous
Where to stand
Your slides can be crutches.
Do not read your slides but they are there if
them
Speak slowly and clearly
you need
If you get lost, do not panic. Take a deep breath
and start again.
Make clear transitions. A few second gap is OK.
Tell one or maybe two stories well, not many.
Do not assume anyone has read your favorite papers
including your own.
RECRUITMENT TALK
THE TALK
• Every slide should have a message and
a reason to be in the talk
• Tell a story. The talk should have a
beginning middle and end.
– Place the topic in context
• Big picture
– Logic flow: A set of slides showing what
you will present: Fade in an out.
– Results
– Conclusion
– Appropriate amount of time
• 45 minutes
RECRUITMENT TALK
• THE TALK
– Are you logical
– Are you careful
– Acknowledge anyone who did the work
on the slide
– Introduction
• Put the topic in context
– Enough background so people understand the
question
– What is the question
– Why is this important
RECRUITMENT TALK
• The four slide cassette
Introduction slide
Set the question
Present a model describing the question
A picture is worth a thousand words!
Method slide
What was done and why
Explain the approach and why
Data slide
Make a new slide for the talk with key points only
NOT from your paper
Controls are critical
Are you careful.
Conclusion/transition slide
What does the data show you
Go back to your model
Present a revised model slide
What is the next question
Set the next cassette
RECRUITMENT TALK
• THE END
• What have you learned
• What are the next questions
• One and maybe two slides
– What would the title of your grant
application be
– What is the model
– What are the aims
RECRUITMENT TALK
• THE DREADED DINNER
– Do not drink AT ALL
• It is too easy to sip too many times.
• If they order you a drink or a glass of wine pick it up put it to your
lips occasionally and do not drink
– Think about what you order
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Order a salad
Main course should be easy to eat
Skip desert
Only order coffee after others
DO NOT order spaghetti.
– Do not discuss politics or religion unless asked and then say
little and try to change the topic
– Nobody cares about how they do things at home
– You must participate
– People like to talk about themselves
• Where are they from. How did they get to the institution. Why did
they come
– Try to sit next to someone you know or the recruit leader
– The Dinner is not a time to ask contentious questions
THE OFFER
• Review carefully
• Ask questions but not too many
– No one wants to work with someone who
is a pain
• Your best chance to negotiate is up
front
– No one wants to work with someone who
is not excited about the job. Too much
negotiation will lose the job
• Produce and renegotiate
• Produce and move elsewhere
WHAT GOES AROUND COMES AROUND:
MULTIPLE GENOMIC AMPLICONS
TARGET VESICLE RECYCLING AND
BIOENERGETICS
Joe Gray William Muller Jim Norman
Gordon B. Mills
Department of Systems Biology
15 years of fishing in
the genome pool
FISHING CAN
WORK IF YOU USE
THE RIGHT BAIT
JOE GRAY LBL AND FRIEND
Early Stage Breast Cancer Resource
(2408 tumors Stage 1,2 paraffin n= 971 MIP arrays) Decision tree analysis
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Melissa Bondy, Patricia Thompson, Spyros Tsavichidis
GENOMIC ABERRATIONS TARGET ENDOCYTOSIS AND POLARITY
Rab25
PKCi
PIK3CA
MDS1 EVI1
Mir
Rab11FIP1
RCP
PVT1
Cyclin D1
Myc
ERBB2
Grb7
ZNF217
RAB11FIPS AND RAB11 FAMILY MEMBERS ARE
OVEREXPRESSED IN CANCER
Bittner Oncomine
RAB25 AND RCP COOPERATE IN LUMINAL B TUMORS
Rab25 is expressed
in ER positive and
HER2 positive, low
in basal, absent in
Claudin low
Ju-Seog Lee
Fan Zhang
RAB25 AND RCP COOPERATE IN LUMINAL B TUMORS
Ju-Seog Lee
Fan Zhang
RAB25 AND RCP REGULATED VESICLE RECYCLING
MEDIATE PLEIOMORPHIC FUNCTIONS
RECYCLING
Specific
Signaling
Complexes
DECISION
Polarity
Proliferation
Migration
Invasiveness
Apoptosis
Cargoes
Integrins
Gluts
EGFR family
Transferrin
DEGRADATION
Jim Norman
RAB25 AND RCP REGULATE MOTILITY AND INVASION
THROUGH SPATIALLY RESTRICTED RECYCLING AND
LOCALIZED SIGNALING
Jim Norman
Rab25 drives extension of invasive pseudopods
•Rab25/a5b1 compartment is located near the tip
GFP-a5 integrin
Cherry-Rab25
Patrick Caswell/Heather Spence
RAB25 INTERACTS WITH EGFR
Roshan Agarwal Kwai Wa Cheng
RAB25 AND RCP REGULATED VESICLE RECYCLING
MEDIATE PLEIOMORPHIC FUNCTIONS
RECYCLING
Specific
Signaling
Complexes
DECISION
RAB11
GDP
RAB25
GTP
DEGRADATION
Polarity
Proliferation
Migration
Invasiveness
Apoptosis
Cargoes
Integrins
Gluts
EGFR family
Transferrin
RAB25 includes a sequence
change found in mutant RAS and
should be GTP bound
Dominant active Rab11 family
member
Rab25 preferentially binds RCP
and integrins in presence of
Rab11
RAB25 AND RCP REGULATED VESICLE RECYCLING
MEDIATE PLEIOMORPHIC FUNCTIONS
RECYCLING
Specific
Signaling
Complexes
DECISION
RAS
Raf
Mek
MAPK
AKT
GSK
3
TGFb
signaling
DEGRADATION
Polarity
Proliferation
Migration
Invasiveness
Apoptosis
Cargoes
Integrins
Gluts
EGFR family
Transferrin
RAB25 AND RCP EXIST AS A HETEROTETRAMERIC COMPLEX
Greg Verdine Stapled peptides
Rab25
Rab25
RCP
RCP
RAB25 INTERACTION NET: ReMTH, Yeast two
hybrid, ip Mass Spec, Lumiere, Literature, STRING
GLUT1
Actin capping
protein
Integrina5 b1
RCP
EGFR
AKT
TGFbR1
Igor Jurisica, Roshan Agarwal, Zhiyong Ding, Kwai Wa Cheng, Jim Norman
RAB25 TRANSCRIPTOME IMPLICATES
RAB25 IN BIOENERGETICS
53-gene signature
Roshan Agarwal Kwai Wa Cheng
GLYCOGEN IS A POTENTIAL
ENERGY SOURCE
AKT
GSK3
GPI
ATP and GLYCOGEN ARE
INCREASED BY RAB25
Kwai Wa Cheng
GLYCOGEN STORES CONTRIBUTE TO THE
ABILITY OF RAB25 TO MAINTAIN ATP LEVELS
Roshan Agarwal Kwai Wa Cheng
Mechanisms of action of Rab25
COLLABORATORS
MILLS LAB
Rab25
Kwai Wa Cheng
Roshan Agarwal
Sofie Claerhout
Yiling Lu
Qinghua Yu
Shuangxing Yu
Shreya Mitra
ReMTH
Zhiyong Ding
Bioenergetics
Jae Ho Cheong
Jiyong Liang
RPPA/PI3K/Patient samples outcomes
Yiling Lu
Bryan Hennessy
Ana Gonzalez
Mark Carey
Fan Zhang
Joe Gray
LBL
Bill Muller
McGill Canada
Jim Norman
Beatson Scotland
Igor Jurisica
University of Toronto
Robert Bast
Ju Seog Lee
MD Anderson
MD Anderson
COLLABORATORS
MDACC
Ju-Seog Lee Prahlad Ram
Gabor Balazsi
Shiaw-Yih “Phoebus” Lin
Georg Halder
Gabriel Hortobagyi
Funda Meric-Bernstam
Robert Bast Cheryl Walker
Steve Kornblau
Melissa Bondy John Heymach
Michael Andreeff
Eric Jonasch
Tissue Bank
Ayesegul Sahin
Karen Lu Ignacio Wistuba
Rosemarie Schmandt
Bioinformatics
Kevin Coombes John Weinstein
Jonas Almeida Keith Baggerly
Mandri Obeyesekere
San Francisco Joe Gray Wenlin Kuo
Boston Joan Brugge
Levi Garraway
John Albeck
Yoko Irie
St. Louis
Chuck Perou
Vanderbilt
Carlos Arteaga
UBC Sam Aparicio
David Huntsman
Baylor Zhou “Sunny” Songyang
Powel Brown Adrian Lee Jenny Chang
Holland
Rene Bernards
Norway Anne-Lise Boerresen-Dale
Therese Sorlie
Spain Jose Baselga
Ana Lluch
Toronto Patricia Shaw Heather Begley
San Francisco Karen Smith McCune
Toronto Igor Jurisica
San Francisco Paul Spellman, William Gibb
Sach Murkherjee
GSK/Semafore/Abbott/Exelixis/Keryx