Raman spectroscopy
Physics
Department
Faculty of
Science
Mr/ Abd El mo’ez Ahmed Mohammed
“3th group”
“Neutron physics and application in
material science and nuclear
application”
physics department
Faculty of Science
Sohag University
Egypt
JINR’s group
Supervisor : prof . S.I. Tutunnikov.
Technician : Mr. Kovalev Yura.
Why we use Raman spectroscopy?
Raman spectroscopy is used to
determine the molecular motions,
especially the vibrational one .
Now what is the application?
We can use Raman spectroscopy in: Tissue characterization.

Tissue Imaging.

Live Cell Imaging.
Tissue characterization
Tissue Imaging
Live Cell Imaging
All the sexiness, without the dyes!!
hn
h(n (-+) n1)
3
hn
2
1
0
Inelastic Scattering
Virtual Level
Energy
• Energy transferred from
incident light to molecular
vibrations
S1
3
2
1
0
Rayleigh
(elastic) Scattering
Raman (inelastic)
Scattering
S0
difference in energy
Raman Spectrometer
Main view of SOLAR-TII setup
Raman spectrometer’s
mechanism.
The laser beam falls on the filter and then it passes to
the objective and collides with the
Sample.
The light will be scattered in all directions and will
be reflected to the objective again.
After the reflected light reaches the objective it falls
down on the spectrometer system and finally to the
CCD or PMT detectors.
Principle of Confocal Microscopy
Experimental data of a ceramic
material.
600
Intensity
400
200
0
-200
400
450
500
550
600
Wave lenght (nm)
650
700
750
Acknowledgement
Finally I’d like to thank
1- Mr/ Kovalev Yura.
2- Mr/ Valantine.
for their help and efforts.