Slide 1 of 9 Update on Pre-Exposure Prophylaxis: Never a Dull Moment Jeanne Marrazzo, MD, MPH Professor of Medicine University of Washington Seattle, Washington From J Marrazzo, MD, at Los Angeles, CA: April 22, 2013, IAS-USA. IAS–USA Slide 2 of 9 What is PrEP, and Why Might it Work? • Definition: Provision of chemopreventive agent at vulnerable site(s) prior to infection • Rationale: • Infection of healthy mucosa requires relatively large dose of virus (10-6 to 10-8 particles) • In theory, the right drug could prevent founder population from establishing infection, but time is of the essence! • Animal data is very supportive; stresses need for PEP Garcia-Lerma JG, et al. Trends Pharmacol Sci. 2010 component From J Marrazzo, MD, at Los Angeles, CA: April 22, 2013, IAS-USA. Slide 3 of 9 Evidence for PrEP Efficacy with Tenofovir-Based Regimens, 2012* Study iPrEx Population N Efficacy (%) Men Having 2499 Sex with Men 44% Daily oral FTC/TDF Young men & 1200 women 62% Daily oral FTC/TDF Partners PrEP Heterosexual 4758 Study couples 67% daily oral TDF 75% daily oral FTC/TDF Brazil, Ecuador, Peru, South Africa, Thailand, US TDF2 Study Botswana Kenya, Uganda • At time of FDA meeting Baeten J, Celum C. Current Opinion HIV AIDS, 2012 From J Marrazzo, MD, at Los Angeles, CA: April 22, 2013, IAS-USA. Slide 4 of 9 FDA Antiviral Drugs Advisory Committee Meeting, May 10, 2012 • Recommended approval of daily TDF/FTC for HIV prevention in 3 groups: – MSM: 19-3 YES – HIV-uninfected partner in discordant couples: 19-2 YES – Other populations: 12:8 YES 16 July 2012 From J Marrazzo, MD, at Los Angeles, CA: April 22, 2013, IAS-USA. Slide 5 of 9 Oral FTC/TDF PrEP Studies Effect size (95% CI) FTC/TDF for HIV discordant couples (Partners PrEP) 75% (55; 87) FTC/TDF for young Heterosexuals (TDF-2) 63% (22; 83) FTC/TDF for MSM (iPrEx) 42% (15; 63) Wide confidence intervals outside of discordant couples, with lower limit <20% in iPrEx 0 10 20 30 Efficacy 40 50 60 70 From J Marrazzo, MD, at Los Angeles, CA: April 22, 2013, IAS-USA. 80 90 100% Slide 6 of 9 Oral FTC/TDF PrEP Studies, 2012 Effect size (95% CI) FTC/TDF for HIV discordant couples (Partners PrEP) 75% (55; 87) FTC/TDF for young heterosexuals (TDF-2) 63% (22; 83) FTC/TDF for MSM (iPrEx) 42% (15; 63) FTC/TDF for women (FEM-PrEP) 6% (-69; 41) Efficacy 0 10 20 30 40 50 60 70 From J Marrazzo, MD, at Los Angeles, CA: April 22, 2013, IAS-USA. 80 90 100% Slide 7 of 9 Divergent PrEP trial results: Contributions of Adherence + Biology? Adherence Biology* • • • • Behavior Low genital drug levels (pK/pD) Inflammation, STI Acute HIV (high VL in partner) Disrupted vaginal microbiome * These factors may make PrEP efficacy more sensitive to imperfect adherence Slide adapted from J. Baeten Efficacy From J Marrazzo, MD, at Los Angeles, CA: April 22, 2013, IAS-USA. • Frequency of anal vs. vaginal intercourse • Vaginal hygiene practices PrEP Slide 8 of 9 HIV Incidence in CAPRISA 004 No K65R resistance mutations among seroconverters Abdool Karim, Q, et al. Science. 2010;329:1168-1174. From J Marrazzo, MD, at Los Angeles, CA: April 22, 2013, IAS-USA. Slide 9 of 9 Candidate Drugs in PrEP Pipeline Phase IIB Phase II • NNRTIs: Dapivirine intravaginal ring • Effectiveness trials for dapivirine ring under way (MTN, IPM) • Entry inhibitors: Maraviroc with NRTI or NNRTI • Oral maraviroc +/- FTC/TDF (HPTN 069) • Maraviroc & dapivirine vaginal ring (IPM, MTN) • NNRTIs: Rilpivirine (TMC 278), long-acting injectable Phase I • Once monthly injections; in phase I safety studies in UK • Integrase strand transfer inhibitors Animal Studies • Macaque & humanized mice protected with topical & oral raltegravir (Dobard CROI 30; Neff PLoS ONE 2010); macaques protected with parenteral long-acting agent (GSK744) (Andrews CROI 2013) • NRTIs: Tenofovir intravaginal ring (Smith CROI 2013) From J Marrazzo, MD, at Los Angeles, CA: April 22, 2013, IAS-USA.