Supplemental Figure 2. A, B: Western blot showing a transient decrease in Akt phosphorylation at 10 minin tamoxifen
(A)- and trifluoperazine (B)-treated T265-2c cells, followed by a rebound in phosphorylation by 3 h. C: Tamoxifen also
induced an increase in the expression of SGK1; this protein is evident as 3 distinct bands, which is consistent with the
fact that 5 variably expressed isoforms of this kinase are generated by alternative mRNA splicing. An increase in SGK1
phosphorylation was also evident 24 h after the initiation of tamoxifen treatment. D: In contrast, trifluoperazine
treatment did not increase SGK1 expression or phosphorylation.