1
Supporting information
Supporting information Table 1. Proteins identified in the membrane proteome.
Proteins
were
classified
according
to
their
function
using
the
MapMan
ontology
(http://mapman.gabipd.org/web/guest/home). The phosphorylation column refers to the availability
(+) or not (-) of phosphorylation information (cf. Supplementary Tables 2 and 3).
Supporting information Table 2. Phosphopeptides and phosphorylation sites.
Phosphorylated amino acids are shown under square brackets. In case of ambiguity for the location,
the amino acid symbol is followed by a number referring to the phosphorylation probability range
for the site [79] : 1, 1 > p ≥ 0.75 ; 2, 0.75 > p ≥ 0.5; 3, 0.5 > p ≥ 0.25; 4, p < 0.25. Data for peptides
shared by different accessions are shown in italics.
Supporting information Table 3. Novel phosphorylation sites.
Novelty of phosphopeptides and sites was inferred from their absence in the PhosPhAt database
(http://phosphat.mpimp-golm.mpg.de/index.html) as from September 2013. All peptides are unique
to their accession in the Tair9 protein database (http://www.arabidopsis.org/index.jsp).
Supporting information Table 4. Types of phosphorylation sites
Phosphorylation sites mapped to peptide amino-acid sequences using Motif-X (http://http://motifx.med.harvard.edu/motif-x.html) at 10-5 significance.
Supporting information Table 5. Quantification of unmodified peptides belonging to phosphoproteins listed in Sup. info. Fig. S1 (black and red) and Fig. 5 (red).
Peptides were quantified after a 45 min- and 120 min- NaCl treatment. Corresponding p-values are
indicated.
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Supporting information Fig. 1. Quantitative behavior of non-ambiguous phosphopeptides
according to a 100 mM NaCl treatment.
Heat map representation of phospho-peptides the abundance of which significantly changes
according to a 100 mM NaCl treatment for 45 min (T45) and 120 min (T120). Phospho-peptides
were quantified by label free MS and clustered using euclidien correlation distance metric
(http;//www.tm4.org/mev). Green and red colors indicate down and up regulation respectively. Grey
colors indicate missing data. Bold red characters indicate novel phosphorylation sites.
Supporting information Fig. 2. Quantitative behavior of phosphorylated forms of major
transporters according to NaCl treatment.
(A) SOS1 and NHX1, (B) AHA1, (C) AHA2, (D) AMT1;1, (E) NRT2;1.