E. coli Gene Expression with
Various Sugars
By: Jack, Allie, Kierstin, Antoun
Background
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Lactose, Sucrose, Maltose are all disaccharides
Lactase breaks apart Lactose
Galactose and glucose are monosaccharides
Question: Do different sugars induce or repress Lactase expression?
Hypothesis: We think that the disaccharides will upregulate lactose because they can fit into the active site of
lactose so it can be broken down.
Experimental Design
Negative Control: Water instead of
lactose
Positive Control: Lactose
Prediction: We predicted that the
absorbance of the samples to which
lactose, sucrose, and maltose were
added would be the highest
because lactase would be
upregulated the most in these
samples.
Data
Results
● Lactose showed significant results
● Lactose upregulates lactase best because lactase has a specific active
site that does not allow other disaccharides or monosaccharides to
bind to.
● Investigate sugars with similar structure to lactase
structions
Slide 1: title slide
Slide 2: background that led to question; state experimental question; state hypothesis and
rationale
Slide 3: depict experimental design with schematics; clarify controls, and state predicted
outcome if hypothesis is right
Slide 4: data with proper labels and variability
Slide 5: conclusion (as it relates to hypothesis) and future directions
-one presenter suggested per slide
Turn in the presentation as a powerpoint file (.ppt or .pptx); no links to Google Slides or
PDFs.